12 results about "Protein Region" patented technology

The invention discloses an inherent disordered protein region prediction method based on multi-modal feature fusion. The method comprises the following steps: firstly, carrying out feature embedding and preprocessing on a protein sequence by utilizing a pre-trained TAPE model, screening and preprocessing existing 544 amino acid physicochemical features in biology, and extracting evolutionary conservative features from the protein sequence; splicing the preprocessed protein embedding feature matrix, the preprocessed amino acid physicochemical feature matrix and the preprocessed evolutionary conservative feature matrix to obtain a multi-modal fusion feature matrix; then, carrying out layer normalization on the multi-modal fusion feature matrix, and carrying out dimensionality reduction on protein embedding features in the multi-modal fusion feature matrix to obtain a multi-modal fusion feature matrix after local dimensionality reduction; performing position coding on the multi-modal fusion feature matrix after local dimension reduction; and finally, inputting the position-coded multi-modal fusion feature matrix into the prediction model, and predicting the residues. According to the method, high complementation and synergistic interaction of multi-modal features are realized, and the prediction precision is improved.

The invention belongs to the technical field of plant genetic engineering, and discloses a coding gene of benzyl benzoate 2-hydroxylase participating in a rice PAL-dependent salicylic acid synthesis pathway and application of the coding gene, and the nucleotide sequence of OSD3 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; according to the invention, an OSD3 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant osd3 of which the SA of a rice leaf is reduced to 20% of that of a wild type is obtained, and an OSD3 overexpression plant is created by constructing an OSD3-pMDC43 overexpression vector.

The invention belongs to the technical field of plant genetic engineering, and discloses a gene for regulating and controlling the endogenous salicylic acid content and disease resistance of rice by using an SA synthetic gene OSD3 participating in rice PAL dependence and application of the gene, and the nucleotide sequence of the OSD3 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; according to the invention, an OSD3 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant osd3 of which the SA of a rice leaf is reduced to 20% of that of a wild type is obtained, and an OSD3 overexpression plant is created by constructing an OSD3-pMDC43 overexpression vector.

The invention belongs to the field of biological medicine and immunology, and particularly relates to a monoclonal antibody of a stable neoepitope of a targeted prostaglandin endoperoxide synthase 1 (PTGS1) protein and application of the monoclonal antibody. Wherein the stable neoantigen epitope is a protein region defined by an amino acid sequence as shown in SEQ ID NO. 1; the stable neoantigen epitope is located at the 271 site to the 282 site of the human PTGS1 protein. The epitope has extremely low mutation rate in people and ovarian cancer tissues, so that high specificity and high stability of the antibody are ensured. Experiments prove that the antibody prepared by the invention has excellent performance in ovarian cancer IHC application, can clearly and remarkably distinguish cancer tissues from normal tissues, and provides a reliable diagnosis basis for pathologists.

The invention relates to the technical field of molecular oncology and drug resistance mechanism research, and discloses an S232F site of acquired drug resistance of a fusion gene STRN3-RARA and application of the S232F site in ATRA. The product is used for detecting the sensitivity of a patient suffering from acute promyelocytic leukemia to all-transretinoic acid (ATRA) treatment or screening whether the patient suffering from acute promyelocytic leukemia is resistant to ATRA treatment, an S232F mutation site is an STRN3-RARA fusion gene coding protein region mutation site, and the nucleotide sequence of the STRN3-RARA fusion gene is shown as SEQ ID NO: 1. The S232F mutation site is located at the 974th nucleotide site of the nucleotide sequence, and the base C is mutated into T. According to the present invention, the S232F mutation site can be adopted as the drug resistance detection marker, the reagent or the kit can be designed according to the STRN3-RARA gene S232F mutation site so as to be used for clinical drug resistance detection, and the gene mutation state of the patient can be monitored so as to guide the personalized drug use.

The invention belongs to the field of plant genetic engineering, and discloses a gene participating in a rice PAL-dependent SA synthesis pathway and application of the gene, and the nucleotide sequence of OSD2 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; the OSD2 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant OSD2 with the SA of rice leaves reduced to 15% of the wild type level is obtained, phenotypic analysis and hormone determination are carried out, a pUbi-OSD2-pMDC32 overexpression vector is constructed, and an OSD2 overexpression plant is created. Based on the fact that OSD2 function deficiency leads to the fact that the SA content of rice is remarkably reduced, the rice is more sensitive to xanthomonas oryzae pv. Oryzae, overexpression of OSD2 has high resistance to xanthomonas oryzae pv. Oryzae, therefore, the function of OSD2 in a rice SA synthesis pathway is analyzed, application of the gene in crop disease resistance improvement is explored, and a new thought can be provided for preventing rice diseases in production.

The invention discloses a plasmid for simultaneously realizing display and secretion, application of the plasmid, a system capable of simultaneously realizing display and secretion and a construction method of the system. The plasmid for realizing cell surface display and cell exocrine comprises a first FC protein coding region and a second FC protein coding region, the first FC protein coding region comprises a first signal peptide, a first FC protein region and an MCS region, the MCS region is used for accessing a target gene, and the second FC protein coding region comprises a second signal peptide, a second FC protein region and a transmembrane domain TM. By utilizing the dimer form of the FC fusion protein, on one hand, the antibody or the functional protein can be expressed on the cell surface, and on the other hand, the corresponding antibody molecule or the functional protein can be secreted in the culture supernatant at the same time.

This invention belongs to the fields of biomedicine and immunology, specifically relating to a monoclonal antibody targeting a stable neoantigen epitope of prostaglandin intraperoxidase 1 (PTGS1) protein and its application. The stable neoantigen epitope is a protein region defined by the amino acid sequence shown in SEQ ID NO.1; the stable neoantigen epitope is located at positions 271-282 of the human PTGS1 protein. This epitope has an extremely low mutation rate in the human population and ovarian cancer tissues, thus ensuring the high specificity and high stability of the antibody. Experiments have demonstrated that the antibody prepared by this invention performs excellently in ovarian cancer IHC applications, clearly and significantly distinguishing cancerous tissue from normal tissue, providing pathologists with a reliable diagnostic basis.

This invention discloses a benzyl alcohol synthesis gene and its application, relating to the field of plant genetic engineering. Specifically, the benzyl alcohol synthesis gene is disclosed as OsCAD6, with the nucleotide sequence SEQ ID NO: 1; the nucleotide sequence encoding the protein region is SEQ ID NO: 2; and the amino acid sequence encoding the protein is SEQ ID NO: 3. By knocking out the OsCAD6 gene in the background of Zhonghua 11 rice using CRISPR / Cas9, a mutant oscad6 was obtained, reducing benzyl alcohol content in rice leaves to less than 30% of the wild type. Phenotypic analysis and metabolite determination were conducted, and the Ubipro-OsCAD6-pMDC32 overexpression vector was constructed to create OsCAD6 overexpression plants. This invention is based on the fact that OsCAD6 loss of function leads to a significant decrease in benzyl alcohol content in rice, while OsCAD6 overexpression leads to a highly significant increase in benzyl alcohol and SA content in rice.

The invention relates to the technical field of microbial drug resistance screening, in particular to helicobacter pylori flhF gene point mutation L112P and application thereof. The invention provides a helicobacter pylori multi-drug resistance related SNP (Single Nucleotide Polymorphism) site, the SNP site is a flhF gene coding protein region mutation site, relative to a cDNA (Complementary Deoxyribonucleic Acid) segment of nucleotide with a sequence shown as SEQ ID NO.1, the SNP site is positioned at the 355th site of the segment, the polymorphism is T > C, and the corresponding 112th amino acid is mutated from L to P. The invention further provides a preparation method of the helicobacter pylori multi-drug resistance related SNP site. A specific probe or a CRISPR (clustered regularly interspaced short palindromic repeats) detection system can be designed aiming at the site of flhF gene L112P, and the kit is used for clinical drug-resistant strain screening and has important significance on rapid diagnosis of multi-drug-resistant helicobacter pylori.

The invention belongs to the field of plant genetic engineering, and discloses a gene participating in a rice PAL-dependent SA synthesis pathway and application of the gene, and the nucleotide sequence of OSD2 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; the OSD2 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant OSD2 with the SA of rice leaves reduced to 15% of the wild type level is obtained, phenotypic analysis and hormone determination are carried out, a pUbi-OSD2-pMDC32 overexpression vector is constructed, and an OSD2 overexpression plant is created. On the basis of OSD2 function deficiency, the SA content of rice is remarkably reduced, the rice is more sensitive to xanthomonas oryzae pv. Oryzae, and OSD2 overexpression has high resistance to xanthomonas oryzae pv. Oryzae.

This invention provides novel immunogenic peptides derived from the X protein and polymerase protein of the hepatitis B virus (HBV). [Solution] An immunogenic peptide consisting of an amino acid sequence having a specific sequence is provided. This peptide contains an epitope that is well conserved among multiple HBV variants and originates from a region in the protein that is essential for viral replication. Furthermore, this novel HBV antigen binds to multiple HLA types and IFN in PBMCs from HBV-degraded individuals. γ An epitope that elicits a response has been identified.