Perylene derivative probe self-assembled fluorescent sensor array for protein distinguishing detection

Abstract

The invention relates to a perylene derivative probe self-assembled fluorescent sensor array for protein distinguishing detection, and belongs to the technical field of sensor arrays. The problems that an existing sensor array for protein distinguishing is complex in construction, and the concentration of protein to be detected needs to be known are solved. According to the invention, single-stranded DNA is utilized to induce aggregation of the perylene probe so as to quench fluorescence of the perylene probe, and protein can interact with a single-stranded DNA / probe compound to cause fluorescence change of the probe; through different changes of fluorescence intensity after different protein solutions are added, a novel fluorescence sensor array based on perylene derivative probe self-assembly is constructed, and nine proteins are successfully distinguished. The construction method disclosed by the invention is simple and rapid in reaction, and can distinguish the protein at a relatively low concentration without knowing the concentration of the protein to be detected.

Description

technical field [0001] The invention belongs to the technical field of sensor arrays, in particular to a fluorescent sensor array for protein differential detection based on the self-assembly of perylene derivative probes. Background technique [0002] The differential detection of proteins is closely related to basic research, disease diagnosis and human health, so it is of great significance. Most traditional protein detection methods are either based on the "key" principle, that is, each protein requires a specific antibody, or require complex procedures and large instruments, resulting in high economic and time costs. In recent years, sensor arrays, as an emerging technology, have been widely used in the field of protein differential detection. This approach relies on multiple non-specific or semi-selective detection units that uniquely interact with each analyte to achieve differential detection of the analyte. [0003] There are many kinds of sensor arrays reported f...

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned traditional protein detection methods are generally based on the "lock and key" principle, that is, each protein requires a specific antibody, while other detection methods have complicated procedures or require large instruments, which are expensive and not convenient for rapid and efficient detection

Although the sensor array method can solve these problems, most of the existing sensor arrays for distinguishing proteins have the problems of cumbersome construction procedures, more sensing elements, and the need to know the concentration of the protein to be tested before detection. Practical applications of arrays pose significant limitations

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