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Method for extracting SOD, CAT, NADH oxidase and ATP in white-rot fungi composite adsorbent simultaneously

A white rot fungus and composite adsorption technology, applied in the field of environmental microorganisms, can solve the problems of complex and difficult extraction methods, and achieve the effects of sufficient extraction, fast grinding speed and good cell crushing effect.

Inactive Publication Date: 2015-01-21
HUNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Because the methods of extracting SOD, CAT, NADH oxidase and ATP are not the same, and the extraction method is complicated, it is difficult to extract the microbial SOD, CAT, NADH oxidase and ATP of the composite adsorbent at the same time.

Method used

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  • Method for extracting SOD, CAT, NADH oxidase and ATP in white-rot fungi composite adsorbent simultaneously

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Embodiment 1

[0031] A method for simultaneously extracting superoxide dismutase (SOD), catalase (CAT), reduced coenzyme I oxidase (NADH oxidase) and ATP in a white rot fungus composite adsorbent under heavy metal stress, comprising the following steps:

[0032] (1) Add 2 g of nitrogen-modified titanium dioxide to 100 mL of sodium alginate solution with a mass fraction of 2% to obtain a mixed solution, and the number of spores in 100 mL is 1.0×10 6 The spore suspension of P. chrysosporium per mL of P. chrysanthemum is added to the above mixed solution, and the mixture is stirred to obtain a white rot fungus composite adsorbent. Add the white rot fungal composite adsorbent dropwise to 200 mL of 3% CaCl with a syringe 2 In the solution, pellets containing P. chrysosporium were prepared. The pellets embedded with P. chrysosporium were added to Kirk inorganic medium (the components of Kirk inorganic medium were 1.2 mmol / L of ammonium tartrate, 56 mmol / L of glucose, and 20 mmol / L of anhydrous s...

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Abstract

The invention discloses a method for extracting SOD, CAT, NADH oxidase and ATP in a white-rot fungi composite adsorbent simultaneously. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously comprises the following steps: adding the white-rot fungi composite adsorbent into a CaCl2 solution to prepare small balls with white-rot fungi being embedded, and carrying out constant temperature shaking culture on the small balls with the white-rot fungi being embedded to obtain a culture solution; adding Cd<2+> into the culture solution for carrying out stress culture, then taking out the small balls with the white-rot fungi being embedded, and carrying out liquid nitrogen grinding to obtain a grinding sample; and adding phosphate buffer into the grinding sample, carrying out ultrasonic treatment in an ice bath, centrifuging to obtain a compound of SOD, CAT, NADH oxidase and ATP. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously has the advantages that heavy metal stress is adopted for improving enzyme activity of SOD, CAT and NADH oxidase and increasing ATP content, a cell breakage effect is good and extraction efficiency is high.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a method for simultaneously extracting SOD, CAT, NADH oxidase and ATP in a white rot fungus composite adsorbent. Background technique [0002] The pollution of heavy metal ions in water has attracted much attention, and biosorption is an effective method to remove heavy metal ions in water. Composite sorbents encapsulating white rot fungi for heavy metal ions in water, such as Cd 2+ , Pb 2+ , Cr 6+ It has a strong removal effect, heavy metal ions are toxic to the composite adsorbent microorganisms, and microorganisms have a certain tolerance to heavy metal ions, which is related to the antioxidant mechanism and phosphoric acid oxidation mechanism of the composite adsorbent. [0003] SOD and CAT are the main enzymes in the endogenous antioxidant defense system of organisms. They are in a dynamic balance with free radicals. When cells are under stress, the o...

Claims

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Application Information

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IPC IPC(8): C12N9/08C12N9/02C07H19/20C07H1/06C12R1/645
CPCC07H1/06C07H19/20C12N9/0036C12N9/0065C12N9/0089C12Y106/03001C12Y111/01006C12Y115/01001
Inventor 谭琼陈桂秋曾光明晏铭陈安伟左亚男黄真真郭志
Owner HUNAN UNIV
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