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Rapid evaluation method of oil field reinjection water sterilizing agent

A technology for oil field re-injection water and evaluation method, which is applied in the field of rapid evaluation of oil field re-injection water bactericides, and can solve the problems of the recurrence of the bactericide evaluation process, the long detection period, and the poor evaluation reproducibility.

Inactive Publication Date: 2017-05-31
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0002] At present, domestic oilfields use SY / T5757-2010 "General Technical Conditions for Oilfield Injection Water Fungicides" in the detection of reinjection water fungicides. This standard has the following disadvantages in the process of use: ①Long detection cycle
②Low detection efficiency
③ Poor evaluation reproducibility
It is difficult to reproduce a fungicide evaluation process because the growth of bacteria in each test water sample will not be the same

Method used

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  • Rapid evaluation method of oil field reinjection water sterilizing agent

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Embodiment Construction

[0016] In order to make the above and other objects, features and advantages of the present invention more comprehensible, the preferred embodiments are listed below and shown in the accompanying drawings in detail as follows.

[0017] like figure 1 as shown, figure 1 It is a flow chart of the rapid evaluation method for oil field reinjection water fungicide of the present invention.

[0018] In step 101, filter the water sample to be tested: connect a 20mL disposable syringe with a 22μm filter head, take 5mL of the water sample to be tested and filter it in the 20mL disposable syringe to retain microorganisms in the water sample to be tested. Then remove the filter head, suck the syringe full of air and then connect the filter head, use air to dry the filter head, repeat 3 times.

[0019] In step 102, clean the filter head: connect a 20mL disposable syringe to the 22μm filter head that has filtered the water sample to be tested, take 5mL cleaning solution to clean the filte...

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Abstract

The invention provides a rapid evaluation method of an oil field reinjection water sterilizing agent. The rapid evaluation method comprises the following steps: 1, filtering a water sample to be detected, and intercepting microorganisms in the water sample to be detected; 2, fetching a lysis solution to lyse microorganisms in the water sample to be detected, and putting into a sample tube with a buffer solution; 3, connecting an ATP detector and a computer; 4, fetching an ATP standard solution, and calibrating luciferase activity by using the ATP detector; 5, fetching the solution in the sample tube in the step 2, and detecting the ATP content of the sample. By the rapid evaluation method of the oil field reinjection water sterilizing agent, the evaluation efficiency of the oil field reinjection water sterilizing agent can be greatly improved and the detection time can be effectively shortened.

Description

technical field [0001] The invention relates to the technical field of oilfield development, in particular to a rapid evaluation method for oilfield reinjection water fungicides. Background technique [0002] At present, domestic oilfields use SY / T5757-2010 "General Technical Conditions for Oilfield Injection Water Fungicides" in the detection of reinjection water fungicides. This standard has the following disadvantages in the use process: ①Long detection cycle. The blank bacterial culture adopts the extinction dilution method, that is, the water sample to be tested that has been added to the culture medium is injected into the test bottle step by step with a sterile syringe for inoculation dilution, and incubated at a constant temperature for 7 days, and then calculated by the positive reaction of the bacterial bottle and the dilution factor The number of bacteria, and then the fungicide evaluation. ② Low detection efficiency. In the above bacterial culture, if the blank...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 徐明明周海刚董晓通李霖王炳升代海鹰陈松
Owner CHINA PETROLEUM & CHEM CORP
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