Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-flux bacterial colony testing chip, system and method

A detection chip, high-throughput technology, applied in chemical instruments and methods, biochemical equipment and methods, microbial determination/inspection, etc. It is not suitable for problems such as micro-injection, so as to reduce human interference factors, improve detection accuracy, and reduce detection costs.

Active Publication Date: 2016-08-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are the following defects: the operation is cumbersome, the test cycle is long, and the test results are of little significance to the quality control of actual production
Micropumps can be used, but micropumps with small size and suitable functions are expensive; syringe pumps and constant pressure pumps can also be used, but this will inevitably cause waste of reagents, which is not suitable for micro-sampling, so microfluidic technology is lost the superiority of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-flux bacterial colony testing chip, system and method
  • High-flux bacterial colony testing chip, system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The reaction principle of using ATP bioluminescence intensity to detect the total number of colonies in water is as follows:

[0037] ATP bioluminescence is accomplished in the presence of luciferase, luciferin and ATP. in luciferase and Mg 2+ Under the action of ATP, luciferin is reduced and activated by the energy provided by ATP, and the activated luciferin combines with luciferase to generate luciferin (L)-AMP complex and release pyrophosphate. Subsequently, under the participation of oxygen, the fluorescein (L)-AMP complex is oxidized to generate electrical excitation, and the electrons in the fluorescein molecule transition from the excited state to the lower energy level to emit photons, resulting in fluorescence. Theoretically, the luminescence intensity is proportional to the number of ATP molecules, and the ATP concentration can be determined by detecting the fluorescence intensity.

[0038] Studies have shown and confirmed that there is a good linear relati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a high-flux bacterial colony testing chip, a high-flux bacterial colony testing system and a high-flux bacterial colony testing method. The testing chip comprises a base material and at least one testing unit distributed on the base material, wherein each testing unit comprises a first mixed reaction tank, a second mixed reaction tank and a testing pool which are sequentially arranged along the first direction, the first mixed reaction tank is provided with an ATP extracting solution sample inlet and a bacteria solution sample inlet, the second mixed reaction tank is provided with an enzyme solution sample inlet, an S-shaped microchannel valve is arranged between the first mixed reaction tank and the second mixed reaction tank, and the second mixed reaction tank is communicated with the testing pool. The ATP concentration is determined by testing the fluorescence intensity, good linear relation is available between the ATP content and the microbe quantity, and the total number of the bacterial colony can be calculated by utilizing the principle that the ATP content is in direct proportion to the bacteria quantity. With the adoption of the micro-fluidic chip, the man-made interference factors are reduced, the attenuation of the fluorescence intensity during the testing process is reduced, and the testing accuracy is improved.

Description

technical field [0001] The application belongs to the fields of biochemical, pharmaceutical and food safety detection, and particularly relates to a high-throughput colony detection chip, a detection system and a detection method. Background technique [0002] The total number of colonies refers to the number of bacterial colonies grown per gram (per milliliter) of sample under certain conditions (such as aerobic conditions, nutrient conditions, pH, culture temperature and time, etc.). The determination of the total number of bacterial colonies in water is an important indicator of water quality monitoring, which directly reflects the bacterial contamination level of the water. An important microbial indicator of dairy products, carbonated beverages, and finished beer is the total bacterial count, which reflects the bacterial contamination level of dairy products, carbonated beverages, and beer. [0003] Domestic testing departments usually use the traditional plate countin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/34C12Q1/06
CPCB01L3/5027B01L3/50273B01L3/502738B01L2200/0636B01L2200/0694C12Q1/06
Inventor 亓琳琳顾志鹏聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com