High-flux bacterial colony testing chip, system and method

A detection chip, high-throughput technology, applied in chemical instruments and methods, biochemical equipment and methods, microbial determination/inspection, etc. It is not suitable for problems such as micro-injection, so as to reduce human interference factors, improve detection accuracy, and reduce detection costs.

Active Publication Date: 2016-08-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are the following defects: the operation is cumbersome, the test cycle is long, and the test results are of little significance to the quality control of actual production
Micropumps can be used, but micropumps with small size and su

Method used

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  • High-flux bacterial colony testing chip, system and method
  • High-flux bacterial colony testing chip, system and method

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Experimental program
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Embodiment Construction

[0036] The reaction principle of using ATP bioluminescence intensity to detect the total number of colonies in water is as follows:

[0037] ATP bioluminescence is accomplished in the presence of luciferase, luciferin and ATP. in luciferase and Mg 2+ Under the action of ATP, luciferin is reduced and activated by the energy provided by ATP, and the activated luciferin combines with luciferase to generate luciferin (L)-AMP complex and release pyrophosphate. Subsequently, under the participation of oxygen, the fluorescein (L)-AMP complex is oxidized to generate electrical excitation, and the electrons in the fluorescein molecule transition from the excited state to the lower energy level to emit photons, resulting in fluorescence. Theoretically, the luminescence intensity is proportional to the number of ATP molecules, and the ATP concentration can be determined by detecting the fluorescence intensity.

[0038] Studies have shown and confirmed that there is a good linear relati...

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Abstract

The invention discloses a high-flux bacterial colony testing chip, a high-flux bacterial colony testing system and a high-flux bacterial colony testing method. The testing chip comprises a base material and at least one testing unit distributed on the base material, wherein each testing unit comprises a first mixed reaction tank, a second mixed reaction tank and a testing pool which are sequentially arranged along the first direction, the first mixed reaction tank is provided with an ATP extracting solution sample inlet and a bacteria solution sample inlet, the second mixed reaction tank is provided with an enzyme solution sample inlet, an S-shaped microchannel valve is arranged between the first mixed reaction tank and the second mixed reaction tank, and the second mixed reaction tank is communicated with the testing pool. The ATP concentration is determined by testing the fluorescence intensity, good linear relation is available between the ATP content and the microbe quantity, and the total number of the bacterial colony can be calculated by utilizing the principle that the ATP content is in direct proportion to the bacteria quantity. With the adoption of the micro-fluidic chip, the man-made interference factors are reduced, the attenuation of the fluorescence intensity during the testing process is reduced, and the testing accuracy is improved.

Description

technical field [0001] The application belongs to the fields of biochemical, pharmaceutical and food safety detection, and particularly relates to a high-throughput colony detection chip, a detection system and a detection method. Background technique [0002] The total number of colonies refers to the number of bacterial colonies grown per gram (per milliliter) of sample under certain conditions (such as aerobic conditions, nutrient conditions, pH, culture temperature and time, etc.). The determination of the total number of bacterial colonies in water is an important indicator of water quality monitoring, which directly reflects the bacterial contamination level of the water. An important microbial indicator of dairy products, carbonated beverages, and finished beer is the total bacterial count, which reflects the bacterial contamination level of dairy products, carbonated beverages, and beer. [0003] Domestic testing departments usually use the traditional plate countin...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/06
CPCB01L3/5027B01L3/50273B01L3/502738B01L2200/0636B01L2200/0694C12Q1/06
Inventor 亓琳琳顾志鹏聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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