Adenosine-triphosphate purity detection method

A technology for detection of adenosine triphosphate and purity, which is applied in the field of substance detection, can solve the problems of inaccurate ATP detection method, difficulty in ATP detection kit and instrument quality control, and achieve the effect of accurate and reliable detection method

Active Publication Date: 2016-11-23
SHANGHAI INST OF MEASUREMENT & TESTING TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a method for detecting the purity of adenosine triphospha...

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 is qualitative to ATP standard substance with nuclear magnetic resonance method

[0047] ATP (adenosine triphosphate) usually exists in the form of disodium salt and contains 3 parts of water of crystallization. The structural formula is shown in the following formula:

[0048]

[0049] The ATP standard substance is qualitatively verified by nuclear magnetic resonance spectroscopy and organic element analysis.

[0050] (1) Instruments and reagents

[0051] Bruker AVANCE III 500M NMR spectrometer.

[0052] Dimethyl sulfoxide, deuterated water.

[0053] (2) Test results

[0054] Dissolve 10mgATP standard substance in 0.3mL D 2 In O, 1024 samples were taken to measure the C13 spectrum.

[0055] Dissolve 5 mg of ATP standard substance in 0.6 mL of dimethyl sulfoxide, and sample 8 times to measure the H1 spectrum.

[0056] According to the nuclear magnetic resonance results, it can be seen that the number and structure of carbon and hydrogen atoms in t...

Embodiment 2

[0057] Embodiment 2 is qualitative with elemental analyzer

[0058] The molecular formula of adenosine triphosphate disodium salt combined with tricrystalline water is: C 10 h 14 o 13 N 5 P 3 Na 2 ·3H 2 O. In order to judge whether the ATP reference material contains other organic substances except a small amount of degradation products, we use two organic element analyzers to detect the content of C, H, and N elements in the ATP reference material. The test results are shown in Table 1:

[0059] Table 1 Detecting results of C, H and N content in ATP standard substance by two elemental analyzers

[0060]

[0061]

[0062] According to the test results of the organic element analyzer, the C, H, and O contents in the sample are very close to the theoretical value of ATP, which proves that the ATP in the ATP sample is in the form of adenosine triphosphate disodium salt with 3 parts of crystal water. According to the content of carbon, hydrogen and nitrogen, no other...

Embodiment 3

[0063] Embodiment 3 Moisture determination

[0064] The ATP standard substance was placed in a desiccator, and after 24 hours of vacuum drying, it was determined by the Karl Fischer method.

[0065] According to "Chinese Pharmacopoeia", the moisture content in the ATP standard substance was determined by the Karl Fischer method. First pre-titrate with anhydrous methanol to the end point, then accurately weigh 3 parts of pure water, titrate to the end point respectively, calculate the average F value of the Karl Fischer reagent (each milliliter of Fischer's solution is equivalent to the weight of water, mg), and save into the titrator.

[0066] Take 6 parts of ATP standard substance samples, each about 0.15g, accurately weighed, dissolve with ethylene glycol-anhydrous methanol (60:40, volume ratio), and titrate to the end point with the calibrated Karl Fischer reagent to obtain The moisture content of the samples was calculated and the average value was calculated. The test ...

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Abstract

The invention discloses an adenosine-triphosphate purity detection method. The adenosine-triphosphate purity detection method includes the following steps that moisture in ATP standard substances is subjected to quantitative detection to obtain the content of the moisture, inorganic impurities in the ATP standard substances are subjected to quantitative detection to obtain the content of the inorganic impurities, ATP is detected with the high performance liquid chromatography, the content of the ATP is calculated with the area normalization method, and then the purity of the adenosine triphosphate in the ATP standard substances is calculated with the following formula; the purity of the adenosine triphosphate is obtained in the mode that the content of the moisture and the content of the inorganic impurities are subtracted from 100%, and the result is multiplied by the ATP content. The adenosine-triphosphate purity detection method is more accurate and reliable, the purity of the ATP standard substances can be accurately and stably measured, the detection method is applied to the field of bacterium rapid detection, quality control of the results of calibration of related instruments and verification and detection of the detection method are guaranteed, and standardization work of the method is advanced.

Description

technical field [0001] The invention relates to the field of substance detection, in particular to a method for detecting the purity of adenosine triphosphate. Background technique [0002] The detection of biological contamination is an important link to ensure food safety and medical safety. Foodborne diseases caused by pathogenic microorganisms are the biggest hidden danger of food safety, and it is estimated that billions of people around the world are infected with foodborne diseases every year. In hospitals, medical devices are contaminated and reused by human blood and body fluids, and the detection of biological contamination is crucial to the control of hospital-acquired infections. [0003] Traditional biological detection methods (direct microscope counting, plate counting, cell labeling, etc.) are suitable for general laboratory detection, but the detection is time-consuming and cumbersome, and is not suitable for timely observation of a large number of samples....

Claims

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Application Information

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IPC IPC(8): G01N30/89G01N30/06
CPCG01N30/06G01N30/89G01N2030/027
Inventor 徐勤刘刚李兰英
Owner SHANGHAI INST OF MEASUREMENT & TESTING TECH
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