Method for continuously separating and purifying valine in fermentation liquor by using simulated moving bed chromatography

A simulated moving bed and chromatographic separation technology, applied in the field of biological products processing, can solve the problems of long time, reduced separation purity and high cost, and achieve the effects of strong specific adsorption capacity, low production intensity and low cost

Inactive Publication Date: 2011-01-19
JIANGNAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] Wuxi Jinghai Amino Acid Co., Ltd. Ning Jianfei and others used membrane separation and industrial chromatographic separation to extract and separate L-valine in the patent No. CN101503366 "Membrane Separation and Industrial Chromatography The method of valine has higher yield and product purity, but there are disadvantages such as low efficiency, long time and high cost in use
In "A Method for Separating and Purifying Valine from Valine Liquid" in Patent No. CN101168512, Peng Qi et al. designed a method for separating valine in a five-zone simulated moving bed, but the method can only ob

Method used

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  • Method for continuously separating and purifying valine in fermentation liquor by using simulated moving bed chromatography
  • Method for continuously separating and purifying valine in fermentation liquor by using simulated moving bed chromatography

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Embodiment 1

[0026] After the fermentation, the concentration of valine is about 30g / L, adjust the pH to 2 with hydrochloric acid, centrifuge at 3000 rpm to obtain the supernatant, add 5% of the weight of the supernatant to decolorize with activated carbon, filter to remove the activated carbon, and concentrate the supernatant in vacuum The post-valine concentration was 80g / L. The diameter of the column in the simulated moving bed is 5cm, the number of columns in the 1-5 area is: 2, 2, 2, 2, 2, and the flow velocity in the 1-5 area is 25, 15, 20, 16, 30 cm / min, the system temperature was controlled at 60°C, and the processing volume of the fermentation serum was 98 mL / min. The concentration of the valine product finally obtained from outlet 1 is 32g / L, the purity reaches 99.5%, and the recovery rate is 80%.

Embodiment 2

[0028] After the fermentation, the valine concentration is about 35 g / L, adjust to pH 5 with hydrochloric acid, centrifuge at 8000 rpm to obtain the supernatant, add 10% of the weight of the supernatant to decolorize with activated carbon, filter to remove the activated carbon, and vacuum the supernatant After concentration, the concentration of valine is 70g / L. The diameter of the column in the simulated moving bed is 1 cm, and the number of columns in the 1-5 zone is: 1, 1, 1, 1, 1, and the flow velocity in the 1-5 zone is 30, 22, 27, 18, 50 cm / min, the system temperature was controlled at 80°C, and the processing volume of the fermentation serum was 3.9mL / min. The concentration of the valine product finally obtained from outlet 1 was 32.8 g / L, the purity reached 99.1%, and the recovery rate was 75%.

Embodiment 3

[0030] After the fermentation, the valine concentration is about 20g / L, adjust to pH3 with hydrochloric acid, centrifuge at 10,000 rpm to obtain the supernatant, add 4% of the weight of the supernatant to decolorize with activated carbon, filter to remove the activated carbon, and concentrate the supernatant in vacuum The post-valine concentration was 75g / L. The diameter of the column in the simulated moving bed is 20cm, the number of columns in the 1-5 zone is: 3, 3, 3, 3, 3, and the flow velocity in the 1-5 zone is 35, 25, 32, 16, 40 cm / min, the system temperature was controlled at 20°C, and the processing volume of the fermentation serum was 2.2 L / min. The concentration of the valine product finally obtained from outlet 1 was 37.8 g / L, the purity reached 99.0%, and the recovery rate was 72%.

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Abstract

The invention discloses a method for continuously separating and purifying valine in fermentation liquor by using simulated moving bed chromatography, and belongs to the technical field of biological product processing. In the method, a simulated moving bed chromatography separating device suitable for the composition characteristic of valine fermentation liquor is designed, water is taken as a mobile phase, and the previously invented resin special for valine separation (disclosed in the Chinese patent 200910231735.4) is taken as a stationary phase. The method can completely remove impurities of alanine and leucine from the valine fermentation liquor, achieves product purity of over 99.7 percent to meet the medicinal standard, is suitable for continuous production, has higher recovery rate and production strength, and overcomes the defects of low purity, low efficiency, high pollution and the like in the conventional valine separation and purification process.

Description

technical field [0001] The invention discloses a method for continuously separating and purifying valine in fermentation broth by using simulated moving bed chromatography, which belongs to the technical field of biological products processing. Background technique [0002] L-valine belongs to branched chain amino acid (BCAA), which is one of the essential amino acids for the human body. Its role in research and therapy is also increasingly recognized. [0003] In recent years, thanks to the efforts of our country's scientific and technological personnel, the upstream technology of my country's fermentation method to produce valine has also made great progress, basically reaching the level of industrialization. However, about 0.5% of alanine and leucine produced during the fermentation of valine have similar chemical properties and are difficult to separate. The finished product produced by traditional separation methods such as ion exchange has low valine content and many...

Claims

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Application Information

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IPC IPC(8): C07C229/08C07C227/40B01D15/18
Inventor 丁彦蕊张伟国蔡宇杰廖祥儒王亚辉李枝玲罗军侠张大兵
Owner JIANGNAN UNIV
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