Zooblast culture medium dry powder composition, culture medium composition and preparation method thereof

A technology for animal cell culture and animal cells, which is applied in the field of cell culture medium dry powder composition, can solve problems such as poor safety, and achieve the effects of low cost, promoting animal cell growth, and small differences

Inactive Publication Date: 2009-03-18
BEIJING SKYWING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing dry powder composition of animal cell culture medium that must be used in conjunction with high-content animal serum

Method used

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  • Zooblast culture medium dry powder composition, culture medium composition and preparation method thereof
  • Zooblast culture medium dry powder composition, culture medium composition and preparation method thereof
  • Zooblast culture medium dry powder composition, culture medium composition and preparation method thereof

Examples

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Embodiment 1

[0044] This example is used to illustrate the animal cell culture medium dry powder composition and its preparation method of the present invention.

[0045] table 3

[0046] components mg / L components mg / L L-alanine 90 Calcium Nitrate Tetrahydrate 50 L-arginine hydrochloride 200 potassium chloride 400 L-Aspartic Acid 20 Anhydrous Magnesium Sulfate 43 L-Asparagine 30 Sodium chloride 6400 L-cysteine ​​hydrochloride 20 Anhydrous Disodium Hydrogen Phosphate 320 L-glutamic acid 130 Ferrous Sulfate Heptahydrate 0.2 L-Glutamine 300 Zinc sulfate heptahydrate 0.3 L-histidine hydrochloride 30 D-glucose 2000 L-Hydroxyproline 10 Glutathione 0.3 L-isoleucine 120 sodium pyruvate 50 L-leucine 30 ascorbic acid 3 L-Lysine Hydrochloride 150 Choline chloride 15 L-Methionine 20 Calcium D-pantothenate 3 L-phenylalanine 30 folic acid 5 L-proline 40 Nicot...

Embodiment 2

[0049] This example is used to illustrate the animal cell culture medium dry powder composition and its preparation method of the present invention.

[0050] Table 4

[0051] components mg / L components mg / L L-alanine 110 Calcium Nitrate Tetrahydrate 60 L-arginine hydrochloride 190 potassium chloride 320 L-Aspartic Acid 10 Anhydrous Magnesium Sulfate 60 L-Asparagine 50 Sodium chloride 7300 L-cysteine ​​hydrochloride 10 Anhydrous Disodium Hydrogen Phosphate 380 L-glutamic acid 100 Ferrous Sulfate Heptahydrate 0.4 L-Glutamine 500 Zinc sulfate heptahydrate 0.8 L-histidine hydrochloride 45 D-glucose 3000 L-Hydroxyproline 5 Glutathione 0.9 L-isoleucine 90 sodium pyruvate 60 L-leucine 70 ascorbic acid 7 L-Lysine Hydrochloride 100 Choline chloride 10 L-Methionine 40 Calcium D-pantothenate 4 L-phenylalanine 50 folic acid 3 L-proline 60 Nicoti...

Embodiment 3

[0054] This example is used to illustrate the animal cell culture medium dry powder composition and its preparation method of the present invention.

[0055] table 5

[0056] components mg / L components mg / L L-alanine 100 Calcium Nitrate Tetrahydrate 40 L-arginine hydrochloride 205 potassium chloride 350 L-Aspartic Acid 15 Anhydrous Magnesium Sulfate 55 L-Asparagine 40 Sodium chloride 7000 L-cysteine ​​hydrochloride 15 Anhydrous Disodium Hydrogen Phosphate 350 L-glutamic acid 120 Ferrous Sulfate Heptahydrate 0.3 L-Glutamine 400 Zinc sulfate heptahydrate 0.5 L-histidine hydrochloride 35 D-glucose 2500 L-Hydroxyproline 8 Glutathione 0.6 L-isoleucine 100 sodium pyruvate 80 L-leucine 50 ascorbic acid 5 L-Lysine Hydrochloride 130 Choline chloride 13 L-Methionine 30 Calcium D-pantothenate 5 L-phenylalanine 40 folic acid 4 L-proline 50 Nicot...

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Abstract

The invention provides a dry powder composition for a zooblast culture medium. Compared with the prior dry powder composition for the zooblast culture medium, the dry powder composition provided by the invention reduces the serum dosage of animals by introduction of recombinant protein or animal origin compositions, the dry powder composition for the zooblast culture medium can be matched with low-content animal serum for use without additional introduction of protein compositions (such as the recombinant protein, plant protein or the animal origin compositions including cytokines and so on), and has the same or better function on promoting the growth of zooblast compared with high-content animal serum, so that the dry powder composition for the zooblast culture medium does not generate side effects along with the protein compositions, and has good safety and low cost. Cells cultured by the dry powder composition for the zooblast culture medium have small difference for different batches, low cost and good safety, are favorable for separating downstream products of the cells, and are suitable to be used for virus hosts, expression vectors and so on of biological products and other biological products.

Description

technical field [0001] The invention relates to a dry cell culture medium composition, a cell culture medium composition and a preparation method thereof, in particular to an animal cell culture medium dry powder composition, an animal cell culture medium composition and a preparation method thereof. Background technique [0002] In the existing animal cell culture, the animal cell culture medium dry powder composition is generally dispersed in a carrier (such as redistilled water) to form a liquid medium composition, and then used in conjunction with animal serum at a final concentration of at least 10% by volume; or The animal cell liquid medium composition without animal serum is used in conjunction with the animal serum with a final concentration of at least 10% by volume; or directly using a liquid medium composition including animal serum with a final concentration of at least 10% by volume can ensure the cell growth Normal growth and passage. [0003] Animal serum, h...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/07
Inventor 张韧陈文庆
Owner BEIJING SKYWING TECH CO LTD
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