Method for preparing swine fever-pseudorabies bigeminal live vaccine and product thereof

A pseudorabies and pseudorabies virus technology, applied in the field of veterinary biological products, can solve the problems of affecting the immune effect, increasing the cost, and missing seeds, and achieving the effects of reducing allergic reactions, increasing costs, and simple preparation process

Inactive Publication Date: 2010-04-07
广东永顺生物制药股份有限公司 +1
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  • Application Information

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Problems solved by technology

However, there are many varieties of vaccines for pigs. In actual operation, it is difficult to rationally arrange multiple injections, multiple doses, and various i...

Method used

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Embodiment Construction

[0025] A method for preparing swine fever and pseudorabies dual live vaccine with passaged cells, comprising the steps of:

[0026] (1) cultivating attenuated strain of classical swine fever virus and attenuated strain of pseudorabies virus with subcultured cells;

[0027] (2) Harvesting the cell culture venom obtained in step (1);

[0028] (3) The two kinds of cell culture venoms obtained in the step (2) are mixed in a certain proportion, and a stabilizer and an antibiotic are added to the mixed solution, and freeze-dried in a vacuum to obtain a dual live vaccine for passage cell swine fever and pseudorabies.

[0029] In a specific preparation embodiment of the present invention, the preparation method of the swine fever and pseudorabies dual live vaccine comprises the following steps:

[0030] (1) Select susceptible cells as cells for seedling production: one of bovine testis cells, chicken embryo fibroblasts, pig testis (ST) passage cells, pig kidney (PK15) passage cells, ...

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Abstract

The invention belongs to the technical field of biological products for veterinary use, in particular to a method for preparing a swine fever-pseudorabies bigeminal live vaccine by using passage cells and a product thereof. The method for preparing the swine fever-pseudorabies bigeminal live vaccine by using the passage cells comprises the following steps: (1) culturing a swine fever lentogen strain and a pseudorabies lentogen strain by the passage cells; (2) harvesting cell culture venoms prepared in the step (1); and (3) mixing two kinds of cell culture venoms prepared in the step (2) in a ratio of 1:2 to prepare the passage cell swine fever-pseudorabies bigeminal live vaccine through cooling and vacuum drying. The invention also provides the swine fever-pseudorabies bigeminal live vaccine prepared by the preparation method. The swine fever-pseudorabies bigeminal live vaccine can be used for immunization, can reduce the workload of the immunization, reduce the number of immunization times, avoid immunologic paralysis caused by frequent immunizations, reduce stress on a swine herd correspondingly, and prevent and control the occurrence of the swine fever and the pseudorabies.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a method for preparing a dual live vaccine of swine fever and pseudorabies by using passaged cells and the product thereof. Background technique [0002] Classical swine fever (CSF) and pseudorabies (PR) are two important infectious diseases that harm the intensive pig industry. They exist widely in pig herds in my country and cause huge economic losses to the pig industry. Immunization is an important measure for the prevention and control of swine fever and pseudorabies. However, there are many varieties of vaccines for pigs. In actual operation, it is difficult to rationally arrange multiple injections, multiple doses, and various immunization procedures. It is cumbersome, time-consuming, laborious, and increases costs. It is also easy to cause missed species, which directly affects the immune effect. Multiple (or multivalent) vaccines a...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/187A61K39/245A61P31/14A61P31/20C12N7/00C12R1/93
Inventor 吴文福林旭野张毓金岑小清游启有任向阳张国丽丘成文杨傲冰赖月辉
Owner 广东永顺生物制药股份有限公司
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