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Triple vaccine special for Muscovy duck

Active Publication Date: 2013-09-04
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of various antibiotics, Muscovy duck parvovirus vaccine and high immune yolk antibody can not control the disease, causing great economic losses to the duck industry

Method used

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Embodiment Construction

[0013] The present invention will be further described below in conjunction with specific examples. (but not a limitation of the invention).

[0014] a. Derived from the attenuated MWCA strain of muscovy duck liver white spot disease (muscovy duck reovirus disease) artificially attenuated, it is made of muscovy duck embryo fibroblasts and chicken embryo fibroblasts for mutagenesis and attenuation , has lost its pathogenicity to one-day-old muscovy ducks, and has the following characteristics (1) Virus titer: MWCA strain is effective against TCID of chicken embryo fibroblast monolayer cells 50 ≥10 -50 / 0.1ml; (2) safety: use 10 feathers of 1-day-old healthy susceptible young muscovy ducks, inject 0.2 milliliters of the poison seed stock solution into the legs of each feather, observe for 20 days, and all should survive; (3) immunogenicity: One-day-old healthy and sensitive Muscovy ducklings, intramuscular injection of 0.2 ml into the legs, the minimum immune dose is 600TCID ...

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Abstract

The invention discloses a triple vaccine special for Muscovy duck, and the triple vaccine special for Muscovy duck uses Muscovy duck liver white-spot disease attenuated MWCA strain, Muscovy duck parvovirus attenuated P1 strain and Muscovy duck goose-origin parvovirus attenuated D strain as seed viruses, and respectively uses SPF chicken embryo fibroblast or Muscovy duck embryo fibroblast spinner cultivation technology to propagate virus and obtain the cell toxic liquid, and after mixing according to a proper proportion, a freeze-drying protective agent is added for freeze drying, and the triple live vaccine of Muscovy duck reovirus disease, Muscovy duck parvovirus disease and Muscovy duck gosling plague special for Muscovy duck is prepared; the triple vaccine can be used in the Muscovy duck culture region where the Muscovy duck reovirus disease, the Muscovy duck parvovirus disease and the Muscovy duck gosling plague are prevalent, and the purpose for preventing and controlling three diseases above can be realized by one-time immunization.

Description

technical field [0001] The invention relates to a triple vaccine for muscovy duck reovirus disease, muscovy duck parvovirus disease and goose blast. Background technique [0002] Muscovy duck reovirus disease (commonly known as "liver white spot disease", "flower liver disease", "white spot disease", "liver spleen white spot disease" and "Musco duck necrotizing hepatitis" etc.) Muscovy duck breeding areas in Putian, Fuqing, Fuchuan, Changle, Fujian Province, Jinhua, Zhejiang, Foshan, Guangdong and other places occurred clinically with soft feet as the main symptom, with a large number of white spots on the surface of the liver and spleen, enlarged kidneys, hemorrhage, and spots on the surface. Yellow streaks are the main pathological changes of infectious diseases. The disease only occurs in muscovy ducks, and the age of onset is 7 to 45 days, especially in young muscovy ducks of 10 to 30 days. The morbidity rate is 30-90%, and the case fatality rate is 60-80%. The undead ...

Claims

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Application Information

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IPC IPC(8): A61K39/29A61K39/23A61P31/20A61P31/14
Inventor 陈少莺林锋强程晓霞陈仕龙朱小丽胡奇林林天龙王劭程由铨
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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