Oral biofilm models and uses thereof

Abstract

The present disclosure provides an oral biofilm model including a substrate including a first surface, a second surface, and a plurality of specimens fixedly attached to the first surface, wherein an oral biofilm is capable of forming on the specimen. The surface roughness of at least one of the specimens of the plurality is less than or greater than a surface roughness of at least a second specimen of the plurality. The oral biofilm model also includes a body having sides and a bottom defining a vessel. The body is adapted to receive the substrate and the plurality of specimens and is further adapted to receive a fluid. Methods of forming oral biofilms and methods for identifying an agent for reducing or inhibiting biofilm formation are also provided.

Description

Background technique [0001] Biofilms are defined as sessile communities embedded in a matrix of extracellular polymers, characterized by cells that are irreversibly attached to the surface or to each other. Biofilm communities can be formed by a single species of microorganisms, but in nature, biofilms almost always consist of a mixture of many bacterial species. For example, more than 500 bacterial species have been identified in a typical dental plaque biofilm. [0002] In vivo plaque initiation and growth and biofilms generally consist of several stages and are complex processes determined by many factors of biological and physicochemical origin. Fluid dynamics also play a role in bacterial attachment and biofilm formation. In the early stages of biofilm formation in mammals, for example, surface topography may also be a major factor in determining bacterial adhesion to surfaces. Due to the increased surface area of ​​rough surfaces, rough surfaces may tend to accumulate...

AI Technical Summary

Problems solved by technology

Furthermore, studies using conventional in vitro biofilm models have demonstrated no association between increased surface roughness and increased biofilm accumulation

Images