Oral biofilm models and uses thereof

A technology of biofilm and oral cavity, which is applied in the directions of biochemical equipment and methods, measurement/inspection of microorganisms, methods of supporting/immobilizing microorganisms, etc., and can solve problems such as increasing surface roughness and biofilm accumulation

Inactive Publication Date: 2017-05-31
COLGATE PALMOLIVE CO
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, studies using conventional in vitro biofilm models have demonstrated no association between increased surface roughness and increased biofilm accumulation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oral biofilm models and uses thereof
  • Oral biofilm models and uses thereof
  • Oral biofilm models and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0052] Example 1. Preparation of tooth enamel samples

[0053] Precut bovine enamel samples were obtained from Bennet Amaechi, DDS, MS, PhD, FDI, Department of Comprehensive Dentistry, University of Texas Health, Cariology, 7703 Floyd Curl Drive, MC 7917, San Antonio, TX 78229-3900 Professor and Director of Science Center. The samples were cast into 38 mm diameter pans using acrylic casting resin to allow the samples to be polished to a mirror finish using a Buehler polisher.

[0054] The samples were visually inspected to ensure that the enamel was fully exposed and free of defects. Each disc has approximately 18 to 20 samples. The samples were divided into three groups: polished, acid etched, acid etched plus brushed with the test toothpaste. Acid etching was done by immersing the specimen in 5% citric acid for 30 seconds. A subset of the acid etched samples were brushed on a Kal-Tech linear brusher using a 1:3 slurry of test toothpaste. An ordinary flat trimming toot...

example 2

[0056] Example 2. Based on surface roughness (polished, acid etched, and acid etched / brushed with test toothpaste) versus enamel Bacterial Attachment to Plasma Samples

[0057] Bacterial Attachment Studies Using a Fixed Volume Dynamic Flow Oral Biofilm Model. In this model, the reaction vessel was a 100x15 mm square polystyrene Petri dish (Electron Microscopy Science). Mount enamel samples on microscope slides using modeling clay. Care was taken to ensure that only the conditioned enamel surface was exposed and that the clay surface with the enamel sample was as flat as possible to minimize sample-to-sample and slide-to-slide variations in turbulent flow. For a particular experimental run, three reaction vessels were used. Each container contained a maximum of 24 enamel samples (two slides of 12 samples each). In Experiment 1, a three-panel study was conducted: rough vs. polished vs. whitening toothpaste. For Experiment 2, a two-panel study was performed: coarse versus...

example 3

[0058] Example 3. Protocol for growing and quantifying diverse microbial biofilms on enamel blocks

[0059] A. Semi-dynamic system preparation

[0060] Cut a polystyrene pipette into two 3.5-inch pieces. Pipette pieces are made to fit snugly inside the square container. The pipette slides were sterilized with a 1:10 bleach solution for 30 minutes, rinsed thoroughly with sterile water, and allowed to dry. Such as image 3 As shown in , samples were embedded (12 / slide) on top of a substrate (microscope slide) containing a uniformly distributed layer of modeling clay. Blocks were layered such that there were differently treated samples in each slide to balance any possible positional effects. Microscope slides were UV sterilized (enamel block side up) for 30 min. Under sterile conditions, such as figure 1 Place two pipette pieces in a square dish (container) containing a small sterile stir bar as shown in . Transfer the microscope slide into the container so that it fi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
surface roughnessaaaaaaaaaa
surface roughnessaaaaaaaaaa
Login to view more

Abstract

The present disclosure provides an oral biofilm model including a substrate including a first surface, a second surface, and a plurality of specimens fixedly attached to the first surface, wherein an oral biofilm is capable of forming on the specimen. The surface roughness of at least one of the specimens of the plurality is less than or greater than a surface roughness of at least a second specimen of the plurality. The oral biofilm model also includes a body having sides and a bottom defining a vessel. The body is adapted to receive the substrate and the plurality of specimens and is further adapted to receive a fluid. Methods of forming oral biofilms and methods for identifying an agent for reducing or inhibiting biofilm formation are also provided.

Description

Background technique [0001] Biofilms are defined as sessile communities embedded in a matrix of extracellular polymers, characterized by cells that are irreversibly attached to the surface or to each other. Biofilm communities can be formed by a single species of microorganisms, but in nature, biofilms almost always consist of a mixture of many bacterial species. For example, more than 500 bacterial species have been identified in a typical dental plaque biofilm. [0002] In vivo plaque initiation and growth and biofilms generally consist of several stages and are complex processes determined by many factors of biological and physicochemical origin. Fluid dynamics also play a role in bacterial attachment and biofilm formation. In the early stages of biofilm formation in mammals, for example, surface topography may also be a major factor in determining bacterial adhesion to surfaces. Due to the increased surface area of ​​rough surfaces, rough surfaces may tend to accumulate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12Q1/18
CPCC12Q1/18C12N1/20G01N1/2806
Inventor 卢恰娜·林奥迪马龙R·萨利文大卫·苏里亚诺阿尔蒂·赖格拉尔夫·彼得·圣塔尔皮亚三世
Owner COLGATE PALMOLIVE CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap