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Rapid fecal occult blood double detection test strip and preparation method thereof

A joint detection technology for fecal occult blood, applied in the field of detection of fecal occult blood markers transferrin and hemoglobin, achieving high sensitivity, cost saving, and easy promotion

Inactive Publication Date: 2017-10-20
安徽深蓝医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this method of detecting two markers of fecal occult blood, transferrin and hemoglobin, in one test strip at the same time has not been reported yet.

Method used

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  • Rapid fecal occult blood double detection test strip and preparation method thereof
  • Rapid fecal occult blood double detection test strip and preparation method thereof
  • Rapid fecal occult blood double detection test strip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: Spray 1.0 mg / ml of transferrin and hemoglobin coating antibodies and goat anti-mouse IgG on the coating film as detection lines T1, T2 and control line C respectively, and dry in an oven at 37°C for 4 hours. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a backing plate, on which a sample pad, a colloidal gold bonding pad, a coating film and a water-absorbing pad are glued in sequence. Cut the affixed board into strips with a width of 4mm, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.

[0041] Example 1: Spray 1.5mg / ml of transferrin and hemoglobin coating antibodies and goat anti-mouse IgG on the coating film, respectively as the detection line T1, T2 and control line C, and dry in an oven at 37°C for 4 hours. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test ...

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PUM

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Abstract

The invention discloses a rapid fecal occult blood dual detection test strip and a preparation method thereof, and relates to the technical field of detection of fecal occult blood markers transferrin and hemoglobin. The test strip is composed of a liner, a sample pad connected in sequence on the liner, a double detection gold standard binding pad, a coating film, and a water-absorbing pad. The double detection gold standard binding pad is sprayed with transferrin-labeled antibody and hemoglobin gold For the target glass fiber membrane, there are invisible detection lines T1 and T2 printed with transferrin antibody and hemoglobin antibody on the coated membrane, and invisible control line C printed with goat anti-mouse IgG. The invention is based on the colloidal gold immunochromatography technology for dual detection of transferrin and hemoglobin. One test paper can simultaneously detect two indicators of transferrin and hemoglobin, and has the characteristics of high sensitivity, short reaction time, convenience and ease of use.

Description

technical field [0001] The invention discloses a rapid fecal occult blood dual detection test strip and a preparation method thereof, and relates to the technical field of detection of fecal occult blood markers transferrin and hemoglobin. Background technique [0002] Fecal occult blood test refers to a small amount of gastrointestinal bleeding, which is invisible to the naked eye, and a small amount of red blood cells are digested and decomposed, so that it cannot be found under a microscope. It is a test for detecting such bleeding. The fecal occult blood test of normal people is negative, but positive results are common in stool tests of patients with bowel cancer, gastrointestinal inflammation or bleeding disorders. Therefore, the fecal occult blood detection test is of great significance for the diagnosis of gastrointestinal bleeding, and it is also an effective means for the general screening, primary screening and monitoring of gastrointestinal tumors. [0003] Ther...

Claims

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Application Information

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IPC IPC(8): G01N33/72G01N33/577G01N33/558
CPCG01N33/721G01N33/558G01N33/577
Inventor 张超陈伟钟友好
Owner 安徽深蓝医疗科技股份有限公司
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