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Fecal occult blood detection kit and preparation method

A technology for detection kits and test strips, applied in the field of fecal occult blood detection kits and preparations, can solve the problems of high false positive rate, many interference factors in chemical methods, easy formation of crystals in reagents, etc., and achieve simple methods, good sensitivity and specificity sexual effect

Inactive Publication Date: 2013-06-26
安徽深蓝医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the existing chemical method is limited by many other interference factors, high false positive rate, and easy crystal formation of reagents formulated with glacial acetic acid, there is an urgent need for a fecal occult blood detection with good specificity, high sensitivity, rapid detection, and simplicity. method

Method used

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  • Fecal occult blood detection kit and preparation method
  • Fecal occult blood detection kit and preparation method
  • Fecal occult blood detection kit and preparation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] The preparation method of the fecal occult blood detection kit includes two major steps: the configuration of the treatment solution and the preparation of the kit. The specific steps are as follows:

[0025] 1) Preparation of treatment solution

[0026] Preparation of A gold standard pad treatment solution: Tris-Hcl (10mM, PH=7.4)+0.5%PVP+0.5%TW-20

[0027] Preparation of B sample pad treatment solution: Tris-Hcl (10mM, PH=7.4)+1%PVP+1.8%S17+0.8%Casein

[0028] C spot membrane antibody dilution preparation: PBS {PB (10mM, PH = 7.4), 0.85% NaCl}

[0029] Preparation of D-labeled antibody diluent: PB (10mM, PH=7.4)

[0030] Preparation of E colloidal gold complex dilution: PB (10mM, PH=7.4)+20% sucrose+1%BSA

[0031] 2) Preparation of the kit

[0032] A treatment of membrane materials:

[0033] a. Treatment of sample pad: The sample pad (SB08) used by generals is soaked with the prepared treatment solution. After fully soaking, it can be dried in a drying room until...

Embodiment

[0056] The verification test of 200 cases of clinical samples was carried out to the fecal occult blood detection kit (hemoglobin detection kit) (colloidal gold immunochromatography) of the present invention by adopting the aforementioned method of use, and the control group used the stool produced by Shanghai Createk Biotechnology Co., Ltd. Occult blood detection kit (colloidal gold method) for detection. The results showed that there was no significant difference between the test product and the control group, which was basically consistent with the test results of similar products already on the market, and met the product listing standards.

[0057]

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Abstract

The invention discloses a fecal occult blood detection kit, which comprises a fecal occult blood detection test paper strip or a test paper card, a sample processing pipe or a feces acquisition mixer and a sampling rod, the test paper strip or the test paper card comprises a detection zone (T) coated with anti-Hb 1 monoclonal antibody and a quality control zone (C) coated with a goat anti mouse polyclonal antibody and a colloidal gold coated with anti-Hb 2 monoclonal antibody. The preparation method of the kit comprises the following steps: preparing a treating liquid, preparing the kit, preparing a colloidal gold compound, spraying metal, cutting, assembling, cutting, packaging and the like. The fecal occult blood detection kit has the advantages of good specificity, high sensitivity, rapid and simple detection, and has no limitation by food and medicine.

Description

technical field [0001] The invention relates to the field of detection kits, in particular to a detection kit for fecal occult blood and a preparation method thereof. Background technique [0002] When there is a small amount of bleeding in the digestive tract, there is no abnormal change in the appearance of the stool, because the red blood cells are dissolved and destroyed, and the red blood cells cannot be found under the microscope. This kind of bleeding that cannot be confirmed under the microscope is called occult blood. Experiments that use chemical or immunological methods to confirm bleeding are called occult blood tests. Usually, in the means of checking whether the stool is bloody, checking for occult blood is the most economical and effective method. This test has important reference value for early detection of gastrointestinal bleeding and screening of gastrointestinal tumors. Therefore, fecal occult blood test is very important for preventive medication. ...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 张超陈奉玲洪莲
Owner 安徽深蓝医疗科技股份有限公司
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