Multi-component direct-competitive immunoassay method for labeling haptens by using quantum dots of micromolecule organic matters

Abstract

The invention provides a multi-component direct-competitive immunoassay method for labeling haptens by using quantum dots of micromolecule organic matters and relates to a multi-component direct-competitive immunoassay technology. The multi-component direct-competitive immunoassay method comprises the following steps of: labeling different target analyte haptens by using the highly-efficient water-soluble quantum dots with different fluorescence emission wavelengths, taking polystyrene magnetic microspheres on which different antibodies are fixed and commonly dispersing corresponding target analyte prototypes as well as the haptens labeled by the corresponding quantum dots in phosphate buffer solution to carry out competitive immune response; and quickly separating the polystyrene magnetic microspheres from a liquid phase in a magnetic field by using a reaction balance system, carrying out fluorescence scanning or multi-wavelength detection on the liquid phase under the excitation of ultraviolet light with the same wavelengths and establishing the multi-component direct-competitive immunoassay method for quickly detecting the haptens labeled by the quantum dots of the various target analyte micromolecule organic matters according to the principle that the ratio of the characteristic fluorescence intensity of labeling the haptens by using the ratio of the fluorescence intensity of the different quantum dots in a prototype system to the fluorescence intensity of corresponding blank control is proportional to the concentration of a corresponding target analyte.

Description

technical field [0001] The invention relates to a new technology for direct competitive immunoassay of nanometer fluorescent probes labeled with different small molecular organic substances (pesticides, drugs, environmental endocrine disruptors) and multi-components. Background technique [0002] Many residues of pesticides, drugs, and environmental endocrine disruptors in the environment and agricultural and livestock products exceed the standard, posing a certain threat to the environment and environmental organisms, and affecting food safety and human health. Strengthening the monitoring of various residues in the environment and food requires efficient and rapid multi-component analysis techniques. [0003] The currently reported multi-residue detection methods for pesticides, drugs, and environmental endocrine disruptors mainly include chromatography and chromatographic mass spectrometry. The use of these methods requires expensive instruments and equipment, specialized...

AI Technical Summary

Problems solved by technology

[0003] The currently reported multi-residue detection methods for pesticides, drugs, and environmental endocrine disruptors mainly include chromatography and chromatographic mass spectrometry. The use of these methods requires expensive instruments and equipment, specialized laboratories and well-trained professionals. High processing requirements, complex process, slow speed, high detection cost, low specificity, difficult to adapt to the requirements of a large number of samples and rapid on-site detection

Existing multi-component immunoassays for small molecule organics usually use different antibodies or antigens to be immobilized on different parts of polystyrene microwell plates, and different immune reactions are carried out through spatial resolution. The washing method realizes solid-liquid separation, and the operation is complicated; or using different markers, such as enzymes, fluoresceins, etc. to label different antibodies or antigens, the reaction conditions and detection methods are difficult to be compatible

Therefore, the true multi-component immunoassay technology for small molecule organics has yet to be established.

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