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Modified lectin wrapped magnetic macromolecular liposome nano microsphere, preparation method and application

A technology of nano-microspheres and magnetic nanoparticles, which is applied in the field of magnetic macromolecular liposome nano-spheres, preparation and application of modified lectin, which can solve the problem of difficulty in maintaining antibody activity, insufficient stability, and low grafting efficiency and other problems, to achieve the effect of convenient preparation and separation, simple preparation method, and strong tumor targeting

Active Publication Date: 2014-05-07
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding lectin or avidin-modified magnetic balls, although there are products on the market at home and abroad (such as Invitrogen Dynal, Shaanxi North American Gene Co., Ltd., etc.), there are still problems such as insufficient stability and single function, such as: lectin The grafting efficiency of such active substances on the surface of magnetic beads is low, it is difficult to maintain the activity of antibodies, etc., and it is difficult to meet the requirements of magnetic beads with high surface antibody content

Method used

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  • Modified lectin wrapped magnetic macromolecular liposome nano microsphere, preparation method and application
  • Modified lectin wrapped magnetic macromolecular liposome nano microsphere, preparation method and application
  • Modified lectin wrapped magnetic macromolecular liposome nano microsphere, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1 Preparation of lentil lectin-hexadecyltrimethylammonium chloride

[0046] Dissolve 10 mg of lentil agglutinin (LCA) in 10 ml of a mixed solution of deionized water and isopropanol (the volume ratio of water and isopropanol is 1:1); Add 100 mg of ammonium chloride slowly to the system; adjust the pH value between 7.0 and 12.0 with NaOH, stir at room temperature for 24 hours, dialyze the reaction solution with deionized water for 3 days, and freeze-dry to obtain lentil agglutinin-ten White powder of hexaalkyl quaternary ammonium salt (LCA-HQ) 12.0mg. Depend on figure 1 It can be seen that both the characteristic absorption peaks of lentil lectin and hexadecyl quaternary ammonium salt exist, indicating that the lentil lectin-hexadecyl quaternary ammonium salt has been successfully prepared.

[0047] Among them, dimethyl hexadecyl epoxypropyl ammonium chloride can be replaced by other long-chain alkyl quaternary ammonium salts, and other long-chain alkyl quatern...

Embodiment 2

[0048] Example 2 Effects of different ratios of lentil lectin-hexadecyl quaternary ammonium salt (LCA-HQ) and cholesterol on the particle size of lentil lectin macromolecular liposomes when using the reverse evaporation method

[0049] Dissolve LCA-HQ and cholesterol in different proportions in dichloromethane, and mix well to obtain solution I; prepare deionized aqueous solution II, in which the ratio of solution I and solution II is 1:2; after mixing the two solutions, Sufficient ultrasonic emulsification, dichloromethane was distilled off under reduced pressure on a rotary evaporator to obtain a lentil lectin macromolecular liposome solution. As can be seen from Table 1, adjusting the mass ratio of LCA-HQ and cholesterol can obtain lentil lectin macromolecular liposomes with different particle sizes, and the particle size distribution of the lentil lectin macromolecular liposomes in aqueous solution is also the same. are relatively uniform.

[0050]

Embodiment 3

[0051] Example 3 Preparation of magnetic macromolecular liposomes encapsulated by lentil lectin

[0052] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0053] Configure FeSO with a mass concentration of 1.0% to 20.0% 4 ·7H 2 O and FeCl 3 A blend of FeSO 4 ·7H 2 O and FeCl 3 The mass ratio of the mixture is 1-2:1, and mechanically stirred under the condition of N2 gas. After mixing, add ammonia water with a mass concentration of 25.0%. %, then add oleic acid with a concentration of 1.0% to 10.0% of the total mass of the entire reaction system, continue to stir and react at room temperature for 3 to 10 hours, then stop the reaction, and obtain Fe after magnetic separation 3 o 4 magnetic nanoparticles. Prepared Fe 3 o 4 The particle size of magnetic nanoparticles is 15.0nm, has superparamagnetism, and the saturation magnetization is 30.0emu / g; where Fe 3 o 4 The magnetic nanoparticles can be replaced by other yttrium ferrite magnetic fluids, cobalt nanopart...

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Abstract

The invention discloses a modified lectin wrapped magnetic macromolecular liposome nano microsphere, a preparation method and an application. The lectin wrapped magnetic macromolecular liposome nano microsphere is macromolecular liposome with a double membrane structure, which is formed by wrapping a magnetic nano particle with modified lectin and lipid after forming a lipid membrane. Different from the traditional magnetic nano microsphere surface grafting modification, the magnetic liposome directly employs a derivative of the active substance lectin, lentil lectin-hexadecyl quaternary ammonium salt to wrap the magnetic nano particle, and the method can control a lectin content of the surface of the magnetic sphere and increase the content of the active substance such as the lectin. The lectin wrapped magnetic macromolecular liposome is applicable to the fields of magnetic resonance imaging (MRI), magnetic marking, magnetic rapid diagnosis, controlled medicine release, genetic vectors, magnetic high-temperature therapy, immunomagnetic microspheres and the like.

Description

technical field [0001] The present invention relates to modified functionalized magnetic nano-microspheres and relevant clinical detection and application, and further refers to a modified lectin-wrapped magnetic macromolecular liposome nano-microspheres, preparation method and application, belonging to functionalized magnetic Preparation and application technology of spheres and liposomes. Background technique [0002] Magnetic nanoparticles (MNP) are a class of intelligent nano-magnetic materials, which not only have the unique properties of nano-materials such as small particle size, large specific surface area, high coupling capacity, but also have magnetic responsiveness and superparamagnetism. Gather and position under a constant magnetic field, and absorb electromagnetic waves to generate heat under an alternating magnetic field. Magnetic polymer microspheres have both the characteristics of magnetic nanomaterials and polymers, and have become one of the current rese...

Claims

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Application Information

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IPC IPC(8): B01J13/02A61K49/18A61K49/14A61K49/00A61K47/42A61K9/127A61K48/00A61K41/00C12Q1/68C12Q1/02C12N15/85G01N33/574G01N21/76
Inventor 梁晓飞陈复华李宗海胡晶莹石必枝
Owner SHANGHAI INST OF ONCOLOGY
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