The invention relates to the field of
neuroscience experiments and
biomedical engineering, and discloses a brain stereotactic method and
system based on a turtle
skull zero point, and the method comprises the following steps: taking a repeatedly recognizable bony mark point of a turtle
skull as a zero point; a
plane parallel to the
reference plane of the brain stereotaxic instrument is established as an XY plane, and an axis parallel to the zero point probe is taken as a Z axis. Further, the invention provides a coordinate
conversion method for converting target point coordinates in a three-dimensional image into a coordinate
system of a stereotaxic instrument, which comprises the following steps of: positioning a zero point and a reference point in the image, determining a Z-axis direction, constructing an orthogonal coordinate base, and calculating
rigid body transformation from an image coordinate
system to a stereotaxic coordinate system; therefore, the three-dimensional displacement, the needle inserting depth and the track parameters for the movement of the positioning arm are output. The device can be used in cooperation with an existing brain stereotaxic instrument suitable for turtles, high-
repeatability positioning of turtle
brain region electrode implantation / injection is achieved, and the consistency and efficiency of cross-individual and cross-batch experiments are improved.