The invention belongs to the application field of traditional Chinese medicine meridian doctrine and acupuncture and moxibustion medical skill, in particular to a three-dimensional meridian and acupoint positioning method under a 3D MAX (Three-dimensional Studio Max) environment, which comprises the following steps of: (1) generating a real three-dimensional image of a human body by adopting a virtual reality technology; (2) constructing 3D meridian and acupoint models on a computer by utilizing a 3D model of the human body; (3) carrying out image combination processing to the real three-dimensional image of the human body and the 3D model of the human body so as to enable the images to be overlapped; and (4) comparing and correcting the meridian and acupoint data of the 3D model of the human body, finally forming the real meridian and acupoint data of the human body, and storing the data into a preset database. According to the invention, on the computer, meridians and acupoints are shown on skeletons, muscle, vessels, nerves, lymph, visceral organs and other related positions of the model in the 3D form by a three-dimensional way, so that the error that human body epidermis needle-inserting points are used as meridians and acupoints by mistakes can be corrected, and the defects of the traditional two-dimensional drawing or three-dimensional reality model in expression of meridians and acupoints of the human body can be overcome.