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35results about How to "Sufficient signal to noise ratio" patented technology

Single-stranded polynucleotide tags

It is one objective of the present invention to obtain reproducible representations of expressed mRNA molecules by exploiting a novel technique that relies on short, single stranded polynucleotide tags. In one preferred embodiment, only one polynucleotide tag is obtained from each mRNA molecule, and relatively simple counting statistics can thus be applied after identification and sampling of the different tags, or a subset of tags being present in the population of representative tags. The tags according to the present invention are preferably single stranded polynucleotide tags obtained by subjecting genetic material derived from a biological sample to at least one site-specific nicking endonuclease capable of i) recognizing a predetermined nucleotide motif comprising complementary nucleotide strands and ii) cleaving only one of said complementary strands in the process of generating the at least one single stranded polynucleotide tag. Accordingly, the present invention demonstrates that nicking endonucleases may advantageously be used for obtaining and isolating ssDNA tags. This novel approach in one embodiment eliminates the occurrence of any linker sequence in the ssDNA tag, and it eliminates the presence of a complementary strand in the isolated polynucleotide tag. The lack of linker sequence in the tag and the lack of any complementary strand serves to reduce the huge complexities associated with the analysis of expressed molecules in a biological sample.
Owner:GENOMIC EXPRESSION

Radio frequency circuit structure for implementing function of converting GNSS satellite signal into baseband signal

The present invention relates to a radio frequency circuit structure for implementing a function of converting a satellite signal of a global navigation satellite system into a baseband signal. The radio frequency circuit structure comprises a channel dividing function module, a plurality of frequency converting function modules, a plurality of local oscillator signal modules, and a plurality of analog-to-digital convertor modules. The channel dividing function module is used for dividing a satellite signal of the global navigation satellite system received by an antenna into satellite signals in a plurality of channels. Each of the frequency converting function modules is used for performing frequency conversion on the satellite signals in the corresponding channels to form near zero-frequency signals. Each of the local oscillator signal module is used for generating a local oscillator signal and outputting the local oscillator signal to the frequency converting function module. By using the radio frequency circuit structure for implementing a function of converting a satellite signal of a global navigation satellite system into a baseband signal, frequency conversion and follow-up signal processing can be performed on the satellite signals in each frequency band separately, the signal quality insured, a sufficient signal to noise ratio is provided for a baseband processing circuit, thereby providing a wider application range.
Owner:COMNAV TECH

Method and device for diagnosing a crankcase ventilation of an internal combustion engine

A method and device for diagnosing a crankcase ventilation of an internal combustion engine, which enable a diagnosis of a malfunction of an actuator of the crankcase ventilation. The internal combustion engine has at least two cylinder banks to which the crankcase ventilation is supplied, and an actuator is provided via whose position the distribution of the crankcase ventilation to the at least two cylinder banks is influenced. In a first position of the actuator, a different distribution of the crankcase ventilation to the at least two cylinder banks comes about than in a second position of the actuator that differs therefrom. Given simultaneously activated operation of the at least two cylinder banks, the actuator is switched over at least one between the two different positions. For at least one of the at least two cylinder banks, a characteristic variable of the internal combustion engine influenced by the crankcase ventilation is determined prior to and following the switchover operation. A minimum value for a change in the characteristic variable of at least one of the at least two cylinder banks and / or the ratio of the characteristic variable between the at least two cylinder banks is specified for the switchover-related change in the distribution of the crankcase ventilation to the at least two cylinder banks. A malfunction of the actuator is detected if at least one change derived from the ascertained values for the characteristic variable undershoots its assigned expected minimum value in quantitative terms.
Owner:ROBERT BOSCH GMBH

Method and device for diagnosing a crankcase ventilation of an internal combustion engine

A method and device for diagnosing a crankcase ventilation of an internal combustion engine, which enable a diagnosis of a malfunction of an actuator of the crankcase ventilation. The internal combustion engine has at least two cylinder banks to which the crankcase ventilation is supplied, and an actuator is provided via whose position the distribution of the crankcase ventilation to the at least two cylinder banks is influenced. In a first position of the actuator, a different distribution of the crankcase ventilation to the at least two cylinder banks comes about than in a second position of the actuator that differs therefrom. Given simultaneously activated operation of the at least two cylinder banks, the actuator is switched over at least one between the two different positions. For at least one of the at least two cylinder banks, a characteristic variable of the internal combustion engine influenced by the crankcase ventilation is determined prior to and following the switchover operation. A minimum value for a change in the characteristic variable of at least one of the at least two cylinder banks and/or the ratio of the characteristic variable between the at least two cylinder banks is specified for the switchover-related change in the distribution of the crankcase ventilation to the at least two cylinder banks. A malfunction of the actuator is detected if at least one change derived from the ascertained values for the characteristic variable undershoots its assigned expected minimum value in quantitative terms.
Owner:ROBERT BOSCH GMBH
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