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Heat controlled electric pole electrogenerated chemiluminescence testing apparatus and testing-pool preparation method

A technology of electrochemistry and luminescence detection, applied in the field of electrochemical detection, can solve the problems of large solution consumption, large volume, large detection pool, etc., and achieve the effects of stable detection system, less sample consumption, and easy access to raw materials

Inactive Publication Date: 2008-04-16
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The thermal control electrodes that have been developed are mainly columnar electrodes (platinum wire electrodes, carbon paste electrodes, etc.), the overall volume of these thermal control electrodes is relatively large, and the detection cell required is relatively large, so the consumption of the solution is relatively large

Method used

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  • Heat controlled electric pole electrogenerated chemiluminescence testing apparatus and testing-pool preparation method

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Embodiment

[0022] Firstly, the conductive glass is cut into small square pieces (14) of 2×2 cm, and according to the shape of the required conductive channel, Vaseline is coated on the cut conductive glass to protect the indium tin oxide coating. then HNO 3 The indium tin oxide coating on the glass surface is corroded by a mixed solution of HCl (1:3 molar ratio) for about 20 minutes, and the indium tin oxide coating not covered by vaseline will be corroded. Remove vaseline at last, clean the ITO electrode, and form as shown in Figure 2, " T " shape conduction channel (15) with indium tin oxide coating (width is 1 centimeter). The "|" channel of the "T"-shaped conductive channel is perpendicular to the midpoint of the "-" channel.

[0023] Use detergent, secondary water, acetone, and secondary water to ultrasonically clean the prepared conductive glass sheet for 5 minutes, then dry it naturally and keep the surface of the conductive glass sheet clean. Use conductive glue (22) to fix the...

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Abstract

The invention provides a detection device of thermal control electrode electrochemiluminescence, which is characterized in that the detection device comprises a detection cell and an electrical heating system. The electrical heating system is connected with working electrodes of the detection cell, the working electrodes are conducting glass electrodes with indium-tin oxide (ITO) coating; the detection cell is a three-electrode system, the three electrodes are respectively connected with an electrochemical workstation which is connected with a computer; the detection cell is provided with a photomultiplier at the lower part; the photomultiplier is connected to a feeble light detector which is connected to the computer; the photomultiplier and the detection cell are both arranged in a dark box. The invention is simple to manufacture and convenient to use. The system is stable and is of low cost, which can facilitate in heating the working electrodes. The integrated thermal control ITO electrode electrochemiluminescence detection system has good sensitivity and repeatability on the detection of amines.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, and more specifically relates to a thermal control electrode electrochemiluminescence detection device and a preparation method for a detection cell. Background technique [0002] Electrochemiluminescence (ECL) generally occurs on or near the electrode surface. Usually, people can change the strength and characteristics of ECL by adjusting the relative position of the electrodes, changing the potential applied to the electrodes and changing the electrode materials to obtain the best results. test results. At present, all studies on ECL are carried out at room temperature, but in fact, the ECL that occurs on the electrode surface is not only related to the diffusion of luminescent substances on the electrode surface, but also closely related to kinetic factors such as the mass transfer rate of luminescent substances. The temperature of the surface has a significant influence on these fact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/66G01N27/28
Inventor 陈国南陈毅挺林振宇姜鹰雁邱彬
Owner FUZHOU UNIV
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