Preparation method of recombinant spider silk protein/silver nano biological wound membrane

A technology of spidroin and silver nanometers, which is applied in bandages, absorbent pads, medical science, etc., can solve problems such as RGD-spider protein that have not yet been seen, and achieve the effects of accelerated healing, significant antibacterial properties, and simple manufacturing processes

Inactive Publication Date: 2013-04-17
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As a wound mask, we hope that it has good biodegradability and good tissue compatibility. According to the structure and functional characteristics of spider silk, Professor Li Min of Fujian Normal University has obtained RGD-spider silk on a large scale by applying genetic engineering technology. Silk protein, because the fiber formed by spidroin is a protein fiber with high tensile str

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Dissolve PVA in H 2 Prepare a PVA aqueous solution with a mass concentration of 10% in O, and prepare a PVA nanofiber film by electrospinning at a voltage of 30kv, a curing distance of 20cm, an extrusion speed of 2mL / h, and a temperature of 45°C;

[0024] (2) Spray 0.3% silver nitrate dilute solution evenly on the PVA nanofiber membrane, and dry it at 95°C for 40 minutes;

[0025] (3) Dissolve pNSR16 in formic acid to form a pNSR16 solution with a mass concentration of 5%, and composite pNSR16 in a silver-containing solution by electrospinning technology at a voltage of 90kv, a curing distance of 10cm, an extrusion speed of 5mL / h, and a temperature of 30°C. The PVA nanofiber membrane was dried at 95°C for 20 minutes to obtain a recombinant spidroin / silver nanobiological wound mask.

Embodiment 2

[0027] (1) Dissolve PVA in H 2 A PVA solution with a mass concentration of 9% was prepared in O, and a PVA nanofiber film was prepared by electrospinning at a voltage of 20kv, a curing distance of 30cm, an extrusion speed of 5mL / h, and a temperature of 30°C;

[0028] (2) Spray 1.0% silver nitrate dilute solution evenly on the PVA nanofiber membrane, and dry it at 75°C for 60 minutes;

[0029] (3) Dissolve pNSR16 in formic acid to prepare a pNSR16 solution with a mass concentration of 20%. By electrospinning technology, pNSR16 was composited in silver-containing The PVA nanofiber membrane was dried at 70°C for 60 minutes to prepare a recombinant spidroin / silver nanobiological wound mask.

[0030] (4) The recombinant spidroin / silver nano-biological wound mask prepared by the present invention is used to repair artificial wounds on the skin of rats. The days required for complete healing of the front and rear wounds on the back of the same rat are respectively: using cotton The...

Embodiment 3

[0032] (1) Dissolve PVA in H 2 A PVA solution with a mass concentration of 9% was prepared in O, and a PVA nanofiber film was prepared by electrospinning at a voltage of 10kv, a curing distance of 10cm, an extrusion speed of 5mL / h, and a temperature of 40°C;

[0033] (2) Spray 0.5% silver nitrate dilute solution evenly on the PVA nanofiber membrane, and dry it at 75°C for 60 minutes;

[0034] (3) Dissolve pNSR16 in formic acid to prepare a pNSR16 solution with a mass concentration of 30%. By electrospinning technology, pNSR16 was composited in silver-containing The PVA nanofiber membrane was dried at 80°C for 80 minutes to prepare a recombinant spidroin / silver nanobiological wound mask.

[0035] (4) When the recombinant spidroin protein / silver nano-biological wound mask prepared by the present invention is used for repairing rabbit skin wounds, the days required for the complete healing of the upper and lower wounds of the same rabbit on the same side of the leg are respectiv...

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Abstract

The invention relates to a preparation method of a recombinant spider silk protein/silver-bearing nano wound membrane. The preparation method comprises the steps of dissolving PVA (Polyvinyl Acetate) in H2O to prepare a PVA aqueous solution, conducting electrostatic spinning to prepare PVA nanofiber membrane, uniformly spraying a silver nitrate weak solution onto the PVA nanofiber membrane, drying, dissolving pNSR 16 in formic acid to prepare a formic acid solution of RGD (Arginine-Glycine-Aspartate)-spider silk protein, electrically spinning the formic acid solution of the RGD-spider silk protein into the PVA nanofiber membrane by an electrostatic spinning technology to form a pNSR 16 composite silver-bearing PVA nanofiber membrane, dyring, and obtaining the silver-bearing nano biological wound membrane. The membrane adopts natural protein materials and has bioactivity; as silver nitrate antibacterial components are introduced, the membrane has a significant antibacterial property and can kill most gram-negative bacteria and gram-positive bacteria; with the adoption of the electrostatic spinning technology, the preparation process is simple; and compared with medical gauze, the number of days required by complete healing of animal skin is reduced by approximately one third.

Description

technical field [0001] The invention relates to a biological wound mask applied to repair and regeneration of damaged skin, in particular to a preparation method of a recombinant spidroin protein / silver-containing nanometer wound mask. Background technique [0002] The treatment of skin damage not only needs to promote skin healing, but also needs to pay attention to sterilization and prevent infection. For small-scale acute injuries in the case of good body repair function, the traditional simple passive coverage to protect the wound is effective. However, for patients with chronic, large-scale or long-term repeated infections and abnormal body repair function, active and effective measures must be taken in order to recover as soon as possible. [0003] Traditional wound varnishes or wound covering materials are mostly self-made by hospitals, such as wound varnishes, which are generally composed of main ingredients, film-forming materials, plasticizers, transdermal absorpt...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/24A61L15/44
Inventor 赵亮李敏陈登龙章文贤
Owner FUJIAN NORMAL UNIV
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