Preparation method of recombinant spider silk protein/silver nano biological wound membrane

Abstract

The invention relates to a preparation method of a recombinant spider silk protein / silver-bearing nano wound membrane. The preparation method comprises the steps of dissolving PVA (Polyvinyl Acetate) in H2O to prepare a PVA aqueous solution, conducting electrostatic spinning to prepare PVA nanofiber membrane, uniformly spraying a silver nitrate weak solution onto the PVA nanofiber membrane, drying, dissolving pNSR 16 in formic acid to prepare a formic acid solution of RGD (Arginine-Glycine-Aspartate)-spider silk protein, electrically spinning the formic acid solution of the RGD-spider silk protein into the PVA nanofiber membrane by an electrostatic spinning technology to form a pNSR 16 composite silver-bearing PVA nanofiber membrane, dyring, and obtaining the silver-bearing nano biological wound membrane. The membrane adopts natural protein materials and has bioactivity; as silver nitrate antibacterial components are introduced, the membrane has a significant antibacterial property and can kill most gram-negative bacteria and gram-positive bacteria; with the adoption of the electrostatic spinning technology, the preparation process is simple; and compared with medical gauze, the number of days required by complete healing of animal skin is reduced by approximately one third.

Description

technical field [0001] The invention relates to a biological wound mask applied to repair and regeneration of damaged skin, in particular to a preparation method of a recombinant spidroin protein / silver-containing nanometer wound mask. Background technique [0002] The treatment of skin damage not only needs to promote skin healing, but also needs to pay attention to sterilization and prevent infection. For small-scale acute injuries in the case of good body repair function, the traditional simple passive coverage to protect the wound is effective. However, for patients with chronic, large-scale or long-term repeated infections and abnormal body repair function, active and effective measures must be taken in order to recover as soon as possible. [0003] Traditional wound varnishes or wound covering materials are mostly self-made by hospitals, such as wound varnishes, which are generally composed of main ingredients, film-forming materials, plasticizers, transdermal absorpt...

AI Technical Summary

Problems solved by technology

[0006] As a wound mask, we hope that it has good biodegradability and good tissue compatibility. According to the structure and functional characteristics of spider silk, Professor Li Min of Fujian Normal University has obtained RGD-spider silk on a large scale by applying genetic engineering technology. Silk protein, because the fiber formed by spidroin is a protein fiber with high tensile strength and high elasticity, it has good biodegradability, superior mechanical properties and good tissue compatibility, and it is well-deserved as an innovative The preferred material for facial masks, the wound mask prepared with RGD-spider silk protein also has the function of cell adhesion, and there is no technical report on the use of RGD-spider silk protein for the preparation of wound mask