Flow micro-sphere method for detecting plastocyte specificity immune body
A technology of flow microspheres and platelets, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited application, high specificity, and low sensitivity, and achieve the effects of convenient operation, high sensitivity, and mature technology
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[0011] Detection steps of the present invention are as follows:
[0012] 1 Microsphere coating: The polystyrene microspheres are coated by physical adsorption method, and the Fab segment remains active by utilizing the hydrophobic effect of the Fc segment of the antibody to adsorb on the microspheres. Resuspend the microspheres before coating, add the microspheres to the mouse anti-human platelet membrane glycoprotein monoclonal antibody diluted in the coating buffer (make the ratio of antibody to microspheres 70ug antibody / ml microspheres), and vortex at room temperature for 2 Hours (or overnight at 4°C with vortex shaking), washed three times with 0.01M PBS / TWEEN, then blocked the microspheres with 5% bovine serum albumin shaking for 2 hours, washed three times with 0.01M PBS / TWEEN, and washed in an isotonic medium containing 0.2% sodium azide Resuspend the microspheres in saline solution to a final concentration of 0.25% microspheres. Store at 4°C. On days 1, 2, 5, 10, 30...
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