Oligo dT primer and method for constructing cDNA library

A DNA molecule and library construction technology, applied in the field of Oligo dT primers and cDNA library construction, can solve the problems of large operation restrictions, small scope of application, and single library construction method.

Active Publication Date: 2012-07-04
盛司潼
View PDF4 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The object of the present invention is to provide a kind of Oligo dT primer, solve the Oligo dT primer in the prior art that the scope of application is small, based on ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oligo dT primer and method for constructing cDNA library
  • Oligo dT primer and method for constructing cDNA library
  • Oligo dT primer and method for constructing cDNA library

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0091] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0092]The type IIs restriction endonuclease of the present invention is a restriction endonuclease whose cutting site is outside the recognition sequence, including but not limited to: Acu I, Alw I, Bbs I, BbV I, Bcc I, BceA I , BciV I, BfuA I, Bmr I, Bpm I, BpuE I, Bsa I, BseM II, BseR I, Bsg I, BsmA I, BsmB I, BsmF I, BspCN I, BspM I, BspQ I, BtgZ I, Ear I, Eei I, EcoP15 I, Fau I, Fok I, Hga I, Hph I, HpyAV, Mbo II, Mly I, Mme I, Mnl I, NmeAIII, Ple I, Sap I, SfaN I and TspDT I, preferably Acu I, Bsg I, EcoP15 I or Mme I.

[0093] The mRNA of the present invention is derived from eukaryotic organisms, including but not limited to animals, plants, fungi and protists.

[0094] An Oligo dT primer, the Oligo dT primer is a single-stranded DNA molec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of molecular biology and provides Oligo dT primer, wherein Oligo dT primer is a single-stranded DNA molecule containing continuous dT sequences and recognition sites for cutting. The invention further provides a method for constructing a cDNA library on the basis of the Oligo dT primer. The Oligo dT primer is excellent in applicability, can be applied to various different library schemes, and can further accurately locate the initiation site of mRNA molecule in poly A tail. The method for constructing the cDNA library can ensure that all mRNA molecules in a sample can show specificity in the constructed cDNA library.

Description

technical field [0001] The invention relates to the field of genetic engineering, and more specifically relates to an Oligo dT primer and a method for constructing a cDNA library based on the Oligo dT primer. Background technique [0002] The analysis of gene expression level plays a crucial role in the study of gene function. Gene expression sequence analysis (serial analysis of gene expression, SAGE) is a technology for rapid analysis of gene expression information, which finds out expression SAGE tag sequences with different abundances, so as to obtain genome expression information nearly completely. SAGE technology and gene chip are currently the two most common gene expression profiling methods. With the development of second-generation sequencing technology, the method of constructing cDNA library and then sequencing the mRNA library by using the high-throughput advantage of second-generation sequencing technology, and then performing gene expression profiling has be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C40B40/08C40B50/06C12N15/10
Inventor 盛司潼
Owner 盛司潼
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap