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Oligo dT primer and method for constructing cDNA library

A DNA molecule and library construction technology, applied in the field of Oligo dT primers and cDNA library construction, can solve the problems of large operation restrictions, small scope of application, and single library construction method.

Active Publication Date: 2012-07-04
盛司潼
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Problems solved by technology

[0012] The object of the present invention is to provide a kind of Oligo dT primer, solve the Oligo dT primer in the prior art that the scope of application is small, based on the Oligo dT primer in the prior art, the operation restriction after forming double-stranded cDNA molecules is large, and the library construction method is applied single question

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  • Oligo dT primer and method for constructing cDNA library

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[0091] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0092]The type IIs restriction endonuclease of the present invention is a restriction endonuclease whose cutting site is outside the recognition sequence, including but not limited to: Acu I, Alw I, Bbs I, BbV I, Bcc I, BceA I , BciV I, BfuA I, Bmr I, Bpm I, BpuE I, Bsa I, BseM II, BseR I, Bsg I, BsmA I, BsmB I, BsmF I, BspCN I, BspM I, BspQ I, BtgZ I, Ear I, Eei I, EcoP15 I, Fau I, Fok I, Hga I, Hph I, HpyAV, Mbo II, Mly I, Mme I, Mnl I, NmeAIII, Ple I, Sap I, SfaN I and TspDT I, preferably Acu I, Bsg I, EcoP15 I or Mme I.

[0093] The mRNA of the present invention is derived from eukaryotic organisms, including but not limited to animals, plants, fungi and protists.

[0094] An Oligo dT primer, the Oligo dT primer is a single-stranded DNA molec...

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Abstract

The invention relates to the field of molecular biology and provides Oligo dT primer, wherein Oligo dT primer is a single-stranded DNA molecule containing continuous dT sequences and recognition sites for cutting. The invention further provides a method for constructing a cDNA library on the basis of the Oligo dT primer. The Oligo dT primer is excellent in applicability, can be applied to various different library schemes, and can further accurately locate the initiation site of mRNA molecule in poly A tail. The method for constructing the cDNA library can ensure that all mRNA molecules in a sample can show specificity in the constructed cDNA library.

Description

technical field [0001] The invention relates to the field of genetic engineering, and more specifically relates to an Oligo dT primer and a method for constructing a cDNA library based on the Oligo dT primer. Background technique [0002] The analysis of gene expression level plays a crucial role in the study of gene function. Gene expression sequence analysis (serial analysis of gene expression, SAGE) is a technology for rapid analysis of gene expression information, which finds out expression SAGE tag sequences with different abundances, so as to obtain genome expression information nearly completely. SAGE technology and gene chip are currently the two most common gene expression profiling methods. With the development of second-generation sequencing technology, the method of constructing cDNA library and then sequencing the mRNA library by using the high-throughput advantage of second-generation sequencing technology, and then performing gene expression profiling has be...

Claims

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Application Information

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IPC IPC(8): C12N15/11C40B40/08C40B50/06C12N15/10
Inventor 盛司潼
Owner 盛司潼
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