Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal

A technology of microfluidic chips and nano-stamps, applied in printing, material inspection products, stamping, etc., can solve the problem that the detection method is not convenient enough, the non-quantitative gold standard test strips are not suitable for on-site detection, bedside detection, and gold standard test strips Insufficient quantitative detection and other problems, to avoid perfusion sample injection operations, reduce edge effects, and avoid sample reflux

Active Publication Date: 2015-11-04
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention aims at the rapid, sensitive and convenient detection requirements of low-concentration substances in trace samples such as blood samples, in order to solve the inconvenient and non-quantitative limitations of existing detection methods: 1) the current detection is aimed at blood samples CRP and its lower concentrations of protein substances and cells require multi-step operations, which are not suitable for rapid on-site detection, bedside detection, etc.; 2) multi-step detection reactions increase errors in each step of operation, micropumps The difference in sample injection control will lead to the deviation of the test results and increase the complexity of the test; 3) The quantitative test of the gold standard test strip is insufficient

Method used

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  • Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal
  • Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal
  • Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal

Examples

Experimental program
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Effect test

Embodiment 1

[0058] figure 1 A schematic structural view of an optical microfluidic chip based on a nanostamp-immobilized monoclonal antibody modified layer according to the first embodiment of the present invention is shown. Such as figure 1 As shown, the optical microfluidic chip includes a lower substrate, an upper cover plate, a calibration tank, a dilution tank, a sampling tank, a channel, a calibration tank, a reaction tank, an exhaust hole, a press valve, a liquid storage bag, and an awl needle. The microfluidic immune chip is used for rapid and sensitive detection of extremely low concentration substances such as protein macromolecules in blood samples, urine samples, saliva and other biological samples.

[0059] Wherein, the microfluidic immune chip based on magnetic particles is an integrated chip prepared based on MEMS technology, magnetic particle enrichment and separation technology, immune technology and F-TIR optical detection principle.

[0060] Among them, the upper cove...

Embodiment 2

[0066] figure 2 A schematic structural view of an optical microfluidic chip based on a nano-stamp immobilized monoclonal antibody modified layer according to the second embodiment of the present invention is shown. Such as figure 2 As shown, the optical microfluidic chip includes a lower substrate, an upper cover plate, a calibration tank, a sample loading tank, a channel, a calibration pool, a reaction pool, an exhaust hole, a press valve, a liquid storage bag and an awl needle. Compared with Example 1, the optical microfluidic chip in this example does not have a dilution tank.

[0067] Both the lower base plate and the upper cover plate are made of transparent plastic plates, which are prepared by hot pressing molding technology, and at the same time, calibration grooves, sample filling grooves, sample feeding vents, and calibration vents are formed on the upper cover plate.

[0068] Channels, calibration pools, and reaction pools are prepared with ultra-thin double-sid...

Embodiment 3

[0072] image 3 It is a schematic structural diagram of an optical microfluidic chip based on a nano-stamp immobilized monoclonal antibody modified layer according to the third embodiment of the present invention, as shown in image 3 As shown, the optical microfluidic chip includes: a lower substrate, an upper cover plate, a liquid storage tank, a sample loading tank, a channel, a reaction pool, a comparison pool, an exhaust hole, a press valve, a liquid storage bag and an awl needle.

[0073] The lower substrate is made of transparent plastic plate, which is prepared by hot pressing molding technology. The upper cover plate is made of hydrophilic single-sided adhesive material, and laser engraving technology is used to form liquid storage tanks, sample feeding tanks, channels, and reaction channels on the hydrophilic single-sided adhesive material. Pools, contrast pools and vents.

[0074] The channel, contrast pool and reaction pool are prepared with ultra-thin double-side...

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Abstract

The invention discloses an optical microfluidics chip for fixing a monoclonal antibody modified layer based on a nanometer seal. The optical microfluidics chip comprises a lower substrate, an upper cover plate, a loading slot, a reaction channel, a reaction pond, a reaction fine channel, a reaction exhaust hole, a calibration groove, a calibration channel, a calibration pond, a calibration fine channel and a calibration exhaust hole, wherein the loading slot, the reaction channel, the reaction pond, the reaction fine channel, the reaction exhaust hole, the calibration groove, the calibration channel, the calibration pond, the calibration fine channel and the calibration exhaust hole are formed in the upper surface of the lower substrate or the lower surface of the upper cover plate or between the lower substrate and the upper cover plate; the loading slot is communicated with the reaction pond through the reaction channel which is subjected to hydrophilic treatment, and the reaction pond is communicated with the reaction exhaust hole through the reaction fine channel; and the calibration groove is communicated with the calibration pond through the calibration channel which is subjected to hydrophilic treatment, and the calibration pond is communicated with the calibration exhaust hole through the calibration fine channel. The optical microfluidics chip further comprises openings which are formed in the upper cover plate and correspond to the loading slot, the reaction exhaust hole, the calibration groove and the calibration exhaust hole in position respectively. Antibody-magnetic particles are fixed to the inner side of a top cover of the reaction pond in advance, and paired peculiar recognition monoclonal antibodies or multi-antibodies are fixed to the bottom side of the reaction pond through nanometer seal pressing or micro-nano sample application in advance.

Description

technical field [0001] The invention relates to the technical field of biosensor detection, and is an optical microfluidic chip based on a nano-stamp immobilized monoclonal antibody modification layer using microfluidic technology, immune technology and nano-magnetic particle technology. Background technique [0002] The development of point-of-care testing (POCT) products has gone through the first generation of qualitative testing using test strips, the second generation of semi-quantitative testing using color charts or semi-quantitative instrument reading, and manual testing. The third-generation full quantitative system, and the fourth-generation technology platform that adopts automation, information and intelligence. A microfluidics chip is a microchip that can complete a biochemical analyzer. It can realize the miniaturization of the original testing instrument and make it a portable instrument for bedside testing. [0003] Microfluidic technology is a systematic sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B41K1/38G01N33/68
Inventor 刘春秀刘昶蔡浩原
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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