The invention discloses an optical pumping rubidium atomic clock based on modulation transfer spectrum frequency stabilization laser, and a preparation method thereof. The optical pumping rubidium atomic clock comprises a narrow linewidth laser, a beam expander, a half wave plate, a polarization beam splitting prism, a phase modulator, a rubidium atomic bubble for laser frequency stabilization, ahigh-speed photoelectric detector, a mixer, a high-speed servo feedback circuit, a radio frequency signal source, a laser driving power supply, a convex lens, an acoustic optical modulator, a rubidiumatomic bubble for obtaining a clock transition spectral line, a crystal oscillator frequency synthesizer and a control circuit part; the radio frequency signal source generates a modulation signal, the modulation signal is input into the phase modulator to perform phase modulation on pumping laser, meanwhile, a demodulation signal is generated to perform mixing demodulation with the signal from the photoelectric detector to obtain an error signal, and the laser driving power supply is controlled by the high-speed servo feedback circuit to realize high-stability narrow linewidth laser based ona modulation transfer spectrum. The laser subjected to frequency stabilization is used as the pumping laser of the optical pumping rubidium atomic clock to obtain a high-performance optical pumping rubidium atomic clock.