Method for preparing molecularly imprinted paper chip sensor through click chemistry technology

Abstract

The invention discloses a method for preparing a molecularly imprinted paper chip sensor through the click chemistry technology. The Adobe Illustrator CS4 is adopted for designing paper chip printing patterns, a wax springing printer is adopted for printing patterns in batches to paper, heating and wax melting are carried out to manufacture a hydrophobic wall, a working electrode, a reference electrode and a counter electrode are printed through the screen printing technology, and gold nanoparticles grow on the paper filter surface by the adoption of a seed growth method. Azide alkyl mercaptan is fixed to the paper fiber gold nano material surface and subjected to a click chemistry reaction with propargyl acrylate, a functional monomer of synthetic organophosphorus pesticide molecularly imprinted polymer is selected, template molecules, the functional monomer, quantum dots, the cross-linking agent, the pore-foaming agent, the initiator and the organic solvent of organophosphorus pesticides are evenly mixed according to a certain substance amount, a polymerization reaction is induced under 365nm ultraviolet light, and a paper fiber surface molecularly imprinted polymer film is prepared. High-selectivity, high-sensitivity, low-cost and real-time-detection for organophosphorus pesticide is achieved by the adoption of a photoinduced electricity chemical method.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue detection, more specifically to the preparation of a photoelectrochemical paper chip sensor capable of detecting organophosphorus pesticides, and the present invention also relates to a method for determining trace pesticide residues in a sample using the molecular imprinting technique . Background technique [0002] Pesticides are a class of organic compounds that are widely used to control or eliminate agricultural and forestry pests and diseases. Due to the wide range of pesticides used, the frequency of use is increasing, and the amount of application is increasing, they have become the most serious pesticides that pollute food. Excessive pesticide residues not only pollute agricultural products and the ecological environment, but also endanger human health and life safety. Organophosphorus pesticides are currently a very widely used pesticide in pesticides. Organophosphorus...

AI Technical Summary

Problems solved by technology

At present, the existing detection or screening methods of organophosphorus pesticide residues mainly include chromatography, enzyme inhibition method, immunological method, etc., but these detection or screening methods have deficiencies.

[0004] Thin-layer chromatography is simple to operate, easy to master, and qualitatively accurate, but the accuracy and sensitivity of quantitative determination are low, so it is a commonly used qualitative determination method

Although the detection sensitivity of chromatography-mass spectrometry is high, the price is relatively expensive, and the detection limit of chromatography is high, which cannot meet the actual needs of trace pesticide residues and export trade detection. Therefore, enrichment is usually required before analysis, complex food and The difficulty in the separation and purification of agricultural products is that the sample matrix is ​​complex, there are many interfering substances, and the pretreatment steps are relatively cumbersome.

[0005] The enzyme inhibition method is easy to operate, fast, and does not require expensive instruments. It is especially suitable for on-site detection and screening of a large number of samples, but the sensitivity is poor, and the sensitivity to various organophosphorus pesticides is different. needs improvement

[0006] The enzyme-linked immunoassay method for detecting organophosphorus pesticide residues has high sensitivity, high accuracy, strong specificity, convenience and quickness, but it has high selectivity for reagents. When detecting small molecular substances, it is necessary to synthesize corresponding artificial antigens. Antigen synthesis is not only a heavy workload and a long cycle, but also pesticides or pesticide metabolites with similar structures may cross-react to varying degrees

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