Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lead ion alternating-current impedance sensor research based on hybridization chain reaction (HCR) and TdT modulation double signal amplification

A technology of hybridization chain reaction and double signal, which is applied in the field of electrochemical impedance sensor and its detection, the preparation and application of lead ion AC impedance sensor, which can solve the problems of electron transfer hindrance, electrochemical impedance signal amplification, etc., and achieve fast detection speed , easy to operate, high sensitivity effect

Active Publication Date: 2020-01-03
NINGBO UNIV
View PDF8 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The electron transfer between the electrode interface and the redox probe is greatly hindered, resulting in a significant amplification of the electrochemical impedance signal
At present, there is no report on the construction of lead ion electrochemical impedance sensor by combining hybridization chain reaction and TdT amplification reaction at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lead ion alternating-current impedance sensor research based on hybridization chain reaction (HCR) and TdT modulation double signal amplification
  • Lead ion alternating-current impedance sensor research based on hybridization chain reaction (HCR) and TdT modulation double signal amplification
  • Lead ion alternating-current impedance sensor research based on hybridization chain reaction (HCR) and TdT modulation double signal amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 sensor

[0026] (1) Au: First, Al with a particle size of 0.05 μm 2 o 3 The suspension was polished on a polishing cloth, then ultrasonically treated in water for 3 min, and then washed slowly with distilled water three times to obtain a clean gold electrode (Au).

[0027] (2) HCR / Au: a. Au was blown dry with nitrogen gas and assembled in HP (3μL, 0.2μM) solution for 6h, then washed slowly with distilled water; then soak the electrode in mercaptohexanol (MCH) (2mM) aqueous solution 24min, wash slowly with distilled water; b. Mix 2 μL 10×Tris reaction buffer, hairpin probe H1 (1 μL, 0.5 μM), hairpin probe H2 (1 μL, 0.5 μM) and drop them on the electrode surface in a , reacted at 35°C for 1h, washed with distilled water, and marked as HCR / Au.

[0028](3) G4 / HCR / Au: a. Control the total volume of TdT reaction solution to 2.5 μL: add TdT buffer, dATP (0.4 μL, 10 mM), dGTP (0.6 μL, 10 mM), TdT (1 μL, 10 U / mL) , mixed evenly, drop on the su...

Embodiment 2

[0031] Embodiment 2 with or without Pb 2+ electrochemical response

[0032] Research on the lead ion AC impedance sensor based on hybridization chain reaction and TdT-regulated dual signal amplification, based on the biosensor prepared in Example 1. See figure 2 , Pb-free 2+ When the sensor is in solution (0.1M KCl+5mM[Fe(CN) 6 ] 3- / 4- ) in the electrochemical impedance response is very small, while in 1nM Pb 2+ The AC impedance response was significantly enhanced in the presence of 2+ detection.

Embodiment 3

[0033] Example 3 Pb 2+ detection

[0034] Research on the lead ion AC impedance sensor based on hybridization chain reaction and TdT regulated dual signal amplification, the preparation steps of the sensor are the same as those in Example 1, in the sensor preparation process (3), the Pb is changed successively 2+ The concentration of Pb 2+ Concentrations of : 0, 0.0001, 0.0004, 0.001, 0.004, 0.01, 0.04, 0.1, 0.4, 1, 4, 10 nM were subsequently used to prepare sensors. Record sensor in solution (0.1M KCl+5mM[Fe(CN) 6 ] 3- / 4- ) in the electrochemical impedance response, according to the experimental results, a series of different concentrations of Pb 2+ Corresponding electrochemical impedance response curve, establish electrochemical AC impedance response size and Pb 2 + The quantitative relationship between the concentrations, according to the quantitative relationship between the two, determine the Pb in the sample to be tested 2+ concentration. Experimental results suc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lead ion alternating-current impedance sensor research based on a hybridization chain reaction (HCR) and TdT modulation double signal amplification. The research comprises the following specific steps: treating Au; modifying with HP; uniformly mixing reaction buffer, a hairpin probe H1 and a hairpin probe H2, and dripping the mixture onto the surface of an electrode; carrying out an HCR; then uniformly mixing TdT buffer, dATP, dGTP and TdT, and dripping the mixture onto the surface of the electrode; carrying out a TdT amplification reaction; and dropwise adding Pb<2+>, hemin, DAB and H2O2 in sequence, and marking as IP / G4 / HCR / Au for electrochemical impedance spectroscopy (EIS) detection. In the sensor preparation process, the influences of a series of sensors prepared by Pb<2+> concentration research on an electrochemical impedance signal are changed. The lead ion alternating-current impedance sensor research has the advantages of high sensitivity, high detection speed, accurate and reliable result and low cost.

Description

technical field [0001] The invention relates to an electrochemical impedance sensor and a detection method thereof, in particular to the preparation and application of a lead ion AC impedance sensor based on hybridization chain reaction and TdT regulation dual signal amplification, and belongs to the technical field of functional biological materials and biosensing. Background technique [0002] Lead (Pb 2+ ) is one of the most toxic heavy metal pollutants, and its accumulation in vital organs and tissues of the human body can cause neurological, reproductive, cardiovascular and developmental disorders. The safe drinking water limit set by the Environmental Protection Agency is 15ppb (0.07μM), while the threshold set by the International Agency for Research on Cancer is lower than 10ppb (48.26nM). Traditional lead ion analysis and detection techniques, such as atomic absorption spectrometry, atomic emission spectrometry, inductively coupled plasma, mass spectrometry, and X-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/12
CPCG01N27/3276G01N27/125
Inventor 胡宇芳张青青胡丹丹詹甜玉王邃郭智勇
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com