Method for detecting antitumor drugs by constructing electrochemical sensor with two ratios in same system

An anti-tumor drug and electrochemical technology, which is applied in the fields of biology, medicine and electrochemistry, can solve the problems of background current interference, reduce the accuracy of the target, etc., and achieve the effect of improving accuracy, mild conditions and simple operation

Active Publication Date: 2016-11-16
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional electrochemical analysis methods using a single electrical signal reduce the accuracy of target determination due to background current interference

Method used

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  • Method for detecting antitumor drugs by constructing electrochemical sensor with two ratios in same system
  • Method for detecting antitumor drugs by constructing electrochemical sensor with two ratios in same system
  • Method for detecting antitumor drugs by constructing electrochemical sensor with two ratios in same system

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Experimental program
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Effect test

Embodiment 1

[0029] The preparation process of two ratiometric electrochemical sensors based on the same electrolyte system and bare glassy carbon electrode (GCE) for the detection of anticancer drugs (DOX) is as follows: figure 1 shown. The specific preparation process is as follows: firstly, the glassy carbon electrode is polished into a mirror surface with 0.3, 0.05 μm alumina powder on the suede, and then ultrasonically cleaned with absolute ethanol and double distilled water for 40 seconds, and dried with nitrogen flow. Secondly, in the electrolytic cell, adding a phosphate buffer solution with a pH of 6.5, adding the prepared different volume concentrations of 200 μM doxorubicin hydrochloride aqueous solution, 0.8 mM methylene blue aqueous solution, and 3 mM ferrocene ethanol solution standard sample, so that The volume of the solution was finally 10mL, and the three were mixed evenly before use; then, the treated bare glassy carbon electrode was used as the working electrode, the sa...

Embodiment 2

[0031]First, the glassy carbon electrode was ground on the suede with 0.3, 0.05 μm alumina powder to form a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water for 60 s, and dried with nitrogen flow. Next, add the phosphate buffer solution that pH is 5.5 in the electrolytic cell, add daunorubicin (DNM) that the different volume concentration that prepares is 300 μ M respectively, the thionine (Th) of 1mM, the ferrocene solution mark of 4mM Then, the treated bare glassy carbon electrode was used as the working electrode, the saturated calomel electrode was used as the reference electrode, and the platinum wire electrode was used as the counter electrode to form three electrodes. After the system was stabilized in the electrolyte solution prepared above for 90s, it was scanned from -1.0V to 0.5V, and the peak current value of the corresponding square wave voltammetry curve was recorded. Finally, the peak current of the different conce...

Embodiment 3

[0033] First, the glassy carbon electrode was ground on the suede with 0.3, 0.05 μm alumina powder to form a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water for 60 s, and dried with nitrogen flow. Next, add the phosphate buffered saline solution that pH is 5.5 in the electrolytic cell, add the daunorubicin hydrochloride (DNR) that is 200 μ M of the different volume concentration that prepares respectively, the Nair blue (NB) of 1mM, the ferrocene of 3mM Solution standard sample, so that the final volume of the solution is 10mL, and the three are mixed evenly before use; then, the treated bare glassy carbon electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum wire electrode is used as the counter electrode. After the three-electrode system was stabilized in the electrolyte solution prepared above for 120s, it was scanned from -0.9V to 0.5V, and the peak cu...

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Abstract

The invention discloses a method for detecting antitumor drugs by constructing an electrochemical sensor with two ratios in a same system. The method specifically comprises the following steps that 1, the antitumor drugs and two electrochemical active substances are added into a phosphate buffer solution to prepare a mixed solution; 2, a bare glassy carbon electrode is taken as a working electrode to be placed in an electrolyte solution containing the mixed solution to be stabilized for a period of time, and then square wave voltammetry detection is conducted; 3, ratios (I1 and I2) of peak currents of the antitumor drugs with the different concentrations to peak currents of the other two electrochemical active substances with the different current peak positions are obtained, linear equations are constructed for the concentrations of the antitumor drugs according to I1 and I2, and then the electrochemical sensor with two ratios can be developed. The method is easy to operate, mild in condition and high in antijamming property, stability and sensitivity, can be used for efficient detection on the anthracycline antitumor drugs in serum and has an important application prospect in the fields of bioanalysis and clinical diagnosis.

Description

technical field [0001] The invention relates to the relevant technical fields of electrochemistry, biology and medicine, and in particular to a method for detecting antitumor drugs by constructing two ratiometric electrochemical sensors with the same system. Background technique [0002] Anthracycline antitumor antibiotics commonly used in clinical practice, such as doxorubicin hydrochloride (DOX), daunorubicin hydrochloride (DNR), daunorubicin (DNM) and epihydrochloride Epirubicin (EPI), mainly by inhibiting the synthesis of nucleic acid in the genetic material of cancer cells, has a killing effect on tumor cells of various growth cycles. Such drugs are widely used clinically to treat a variety of malignant tumors, not only for the treatment of acute and chronic leukemia and malignant lymphoma, but also for the treatment of solid tumors such as breast tumors and thyroid tumors. However, such drugs often produce side effects such as inhibition of bone marrow hematopoietic f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/27G01N27/48
CPCG01N27/27G01N27/48
Inventor 桂日军赵春芹金辉王宗花徐显朕
Owner QINGDAO UNIV
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