Oil-tea camellia husk polysaccharide and application thereof

A technology of Camellia oleifera polysaccharide and Camellia oleifera is applied in the field of Camellia oleifera Polysaccharide and its application, which can solve the problems such as no reports on the extraction of Camellia oleifera Polysaccharide, and achieve the effects of being beneficial to environmental protection, reducing resource waste, and being effective in medicines.

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the extraction and research of polysaccharides from Camellia oleifera

Method used

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  • Oil-tea camellia husk polysaccharide and application thereof
  • Oil-tea camellia husk polysaccharide and application thereof
  • Oil-tea camellia husk polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. A preparation method of Camellia oleifera polysaccharide, followed by the following steps:

[0041] 1). Accurately weigh 20g of Camellia oleifera powder that has passed through a 50-mesh sieve, and put the above powder into 400mL of distilled water at a material-to-liquid ratio of 1g / 20mL, and extract for 2 hours at a temperature of 80°C; and then extract together according to the above conditions. Two times, the extract was cooled and centrifuged at 4000 r / min; the supernatants obtained from the above two extractions and centrifugation were combined, and vacuum-concentrated at 60°C to 1 / 6 of its original volume to obtain a concentrated solution;

[0042] 2). Add ethanol solution to the above concentrated solution to control the final volume concentration of ethanol to 80%, that is, ethanol / (concentrated solution + ethanol solution) = 80%; overnight precipitation treatment in a refrigerator at 4°C (ie, the precipitation time is 20 hours ), and then centrifuged at...

Embodiment 2

[0043] Example 2. A preparation method of Camellia oleifera polysaccharide. The extraction method in Example 1 was changed to: in an ultrasonic circulating extractor at a temperature of 80°C, 1000w auxiliary extraction for 0.5 hours; a total of 2 extractions, and the rest are the same Example 1.

[0044] 1.48g of powdered Camellia oleifera polysaccharide was obtained.

Embodiment 3

[0045]Example 3, a method for preparing Camellia oleifera polysaccharide, the extraction method in Example 1 was changed to: under the condition of a microwave power of 2000w, extraction for 30s; a total of 2 extractions, and the rest are the same as in Example 1.

[0046] 1.63g of powdered Camellia oleifera polysaccharide was obtained.

[0047] From the results of Example 1 to Example 3, we can know that the use of microwave and ultrasound helps to improve the extraction rate of polysaccharides.

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Abstract

The invention discloses an oil-tea camellia husk polysaccharide which has the following characteristics: a. physicochemical properties: the polysaccharide is brown powder with small odor and is easily-soluble in water and not soluble in organic solvents such as chloroform and ether, the polysaccharide is orange if reacting with phenol-sulfuric acid, shows a negative reaction with Fehling reagent and shows a negative reaction with iodine-potassium iodide; b. composition of the polysaccharide: the content of the polysaccharide is 30 percent to 50 percent; the distribution range of molecular weight is 7,000 to 200,000,0Da; the polysaccharide is prepared by the following six kinds of monosaccharide: glucose, fucose, arabinose, galactose, seminose and rhamnose. The oil-tea camellia husk polysaccharide is used as free radical scavenger or used for preparing medicaments with anti-tumor function for curing tumors.

Description

Technical field [0001] The invention relates to an extract of Camellia oleifera and its use; more specifically, to a polysaccharide of Camellia oleifera and its use. Background technique [0002] Camellia oleifera (Camellia oleifera Abel) is a woody plant in the Camellia genus (Camellia) of the Camellia family (Theaceae). It is a unique oil tree species in my country, mainly distributed in Jiangxi, Hunan, Zhejiang, Guangxi, Guangdong, Fujian, Guizhou and other places in China. Camellia oleifera can be divided into white-flowered oleifera, safflower oleifera and yellow-flowered oleifera according to different flower colors. The most widely planted is white oleifera, and ordinary oleifera is the most widely distributed. Camellia oil is rich in unsaturated fatty acids and has high nutritional value. It is known as the olive oil of the East. At present, the planting area of ​​Camellia oleifera in China is increasing year by year, and the output of Camellia oleifera seeds is also grad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61K31/715A61P35/00A61P39/06
Inventor 沈建福康海权吴晓琴陈秋平陈亚琪罗晓伟戴甜甜
Owner ZHEJIANG UNIV
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