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Oil-tea camellia husk polysaccharide and application thereof

A technology of Camellia oleifera polysaccharide and Camellia oleifera is applied in the field of Camellia oleifera Polysaccharide and its application, which can solve the problems such as no reports on the extraction of Camellia oleifera Polysaccharide, and achieve the effects of being beneficial to environmental protection, reducing resource waste, and being effective in medicines.

Inactive Publication Date: 2011-04-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the extraction and research of polysaccharides from Camellia oleifera

Method used

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  • Oil-tea camellia husk polysaccharide and application thereof
  • Oil-tea camellia husk polysaccharide and application thereof
  • Oil-tea camellia husk polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, a kind of preparation method of camellia oleifera polysaccharide, carries out following steps successively:

[0041] 1) Accurately weigh 20g of camellia oleifera powder passed through a 50-mesh sieve, put the above powder into 400mL of distilled water according to the ratio of solid to liquid of 1g / 20mL, and extract at 80°C for 2 hours; co-extract according to the above conditions 2 times, the extract was cooled and then centrifuged at 4000r / min; the supernatant obtained from the above 2 times of extraction and centrifugation was combined, and vacuum concentrated at 60°C to 1 / 6 of its original volume to obtain a concentrate;

[0042] 2), add ethanol solution in above-mentioned concentrated solution, control the final volume concentration of ethanol to be 80%, i.e. ethanol / (concentrated solution+ethanol solution)=80%; Overnight precipitation treatment in 4 ℃ of refrigerators (that is, precipitation time is 20 hours ), and then centrifuged at 5000r / min, the ...

Embodiment 2

[0043] Embodiment 2, a preparation method of camellia oleifera polysaccharide, changing the extraction method in embodiment 1 to: in an ultrasonic circulation extractor at a temperature of 80°C, 1000w auxiliary extraction for 0.5 hours; a total of 2 extractions, the rest are the same Example 1.

[0044] Obtain 1.48g of powdered Camellia oleifera polysaccharide.

Embodiment 3

[0045] Embodiment 3, a preparation method of Camellia oleifera polysaccharide, the extraction method in embodiment 1 is changed to: under the condition of microwave power 2000w, extract for 30s; extract 2 times in total, and the rest are the same as embodiment 1.

[0046] Obtain 1.63g of powdered Camellia oleifera polysaccharide.

[0047] From the results of Examples 1 to 3, we can know that the use of microwaves and ultrasound helps to improve the extraction rate of polysaccharides.

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Abstract

The invention discloses an oil-tea camellia husk polysaccharide which has the following characteristics: a. physicochemical properties: the polysaccharide is brown powder with small odor and is easily-soluble in water and not soluble in organic solvents such as chloroform and ether, the polysaccharide is orange if reacting with phenol-sulfuric acid, shows a negative reaction with Fehling reagent and shows a negative reaction with iodine-potassium iodide; b. composition of the polysaccharide: the content of the polysaccharide is 30 percent to 50 percent; the distribution range of molecular weight is 7,000 to 200,000,0Da; the polysaccharide is prepared by the following six kinds of monosaccharide: glucose, fucose, arabinose, galactose, seminose and rhamnose. The oil-tea camellia husk polysaccharide is used as free radical scavenger or used for preparing medicaments with anti-tumor function for curing tumors.

Description

technical field [0001] The invention relates to an extract of camellia oleifera and its use; more specifically, it relates to a kind of camellia oleifera polysaccharide and its use. Background technique [0002] Camellia oleifera (Camellia oleifera Abel) is a woody plant of the genus Camellia in the family Theaceae. It is a unique oil tree species in my country. It is mainly distributed in Jiangxi, Hunan, Zhejiang, Guangxi, Guangdong, Fujian, Guizhou and other places in China. Camellia oleifera can be divided into white-flowered camellia, safflowered camellia and yellow-flowered camellia according to the color of the flowers. The most widely planted is white-flowered camellia, and ordinary camellia is the most widely distributed. Camellia oil is rich in unsaturated fatty acids and has high nutritional value. It is known as the olive oil of the East. At present, the planting area of ​​camellia oleifera in China is increasing year by year, and the output of camellia oleifera ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61K31/715A61P35/00A61P39/06
Inventor 沈建福康海权吴晓琴陈秋平陈亚琪罗晓伟戴甜甜
Owner ZHEJIANG UNIV
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