Nephrotropic avian infectious bronchitis HN99 strain virus

A bronchitis and infectious technology, applied in the directions of viruses/phages, microorganisms, biochemical equipment and methods, etc., can solve the problem of inability to prevent renal infectious bronchitis

Inactive Publication Date: 2008-01-09
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

with traditional M 41 HN strain or H120 strain, H52 strain vaccine can not prevent HN 99 Renal infectious bronchitis caused by strains of virus can only be treated with HN 99 Infectious Bronchitis Renal Type

Method used

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  • Nephrotropic avian infectious bronchitis HN99 strain virus
  • Nephrotropic avian infectious bronchitis HN99 strain virus
  • Nephrotropic avian infectious bronchitis HN99 strain virus

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Experimental program
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Embodiment Construction

[0014] The present invention adopts the following methods to isolate, cultivate and identify viruses:

[0015] (1) Virus isolation and culture

[0016] Aseptically collect diseased chicken lungs and kidneys, weigh them, add sterilized normal saline to grind at a weight ratio of 1:5, centrifuge at 3000 rpm for 15 minutes, take the supernatant, add 2000 IU of penicillin and 2000 μg of streptomycin per milliliter, 4~ 8°C for 12 hours and set aside. Take the supernatant allantoic cavity and inoculate 10 10-day-old SPF embryos, 0.2ml per embryo, and remove the chicken embryos that died within 30 hours. From 30 hours after inoculation, observe once every 4 to 6 hours, pick out the dead embryos, put them in cold storage at 2 to 8°C, take out all the embryos at 72 hours, collect the allantoic fluid aseptically, add penicillin and streptomycin 1000IU / ml. The remaining 5 embryos continued to hatch for 5 days, and the lesions of the embryos were observed and recorded. Blind passage f...

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Abstract

A fowl-kidney-form infectious bronchitis HN99 virus has excellent immunogenicity. The diameter of coronal virus particle is 90-105 nm, it has membrane and is radiant with 18 nm vomer protrusion, it has no interference for NDV La Sota virus multiplying and no agglutination for 1% fowl red blood cell; regression flow experiment leads to respiratory tract symptom and renal boss. Ill fowl virus isolate has positive reaction with HN99 positive blood serum, has negative reaction with NDV La Sota strain, IBDV B87 strain and AIV H9N2 strains; HN99 positive blood serum has positive reaction with T strains, Hubei strain, Tianjin strain and Shandong strain and has negative reaction with other viruses. It has exogenous pathogenic pollution.

Description

technical field [0001] The invention relates to a poultry infectious virus, in particular to a chicken kidney type infectious bronchitis virus. Background technique [0002] In 1999, a chicken farm in Xingyang, Henan Province broke out an infectious disease characterized by respiratory symptoms and anatomical features of kidney swelling and urate deposition. A strain of virus was isolated from the kidneys and ureters of typical cases of chickens, code-named for HN 99 strain. Through the passage of SPF chicken embryos, after the return of SPF chicks to rejuvenation, measure their EID 50 for 10 -6.40 / 0.1ml; We conducted histological observations on the kidneys of diseased chickens, observed the shape and size of the coronavirus particles with an electron microscope, carried out biological characteristic tests such as hemagglutination, interference tests on NDV, and determined Sensitivity to heat, acid, chemical reagents (chloroform, ether, etc.) and other physical and che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 崔保安舒德广张素梅高文明王莉徐端红魏战勇王学斌
Owner HENAN AGRICULTURAL UNIVERSITY
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