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Enzyme sensor including a water-containing spacer layer

A divalent manganese ion, sensor technology, applied in biochemical equipment and methods, instruments, scientific instruments, etc., can solve the problems of unstable zero-point current of electrochemical sensors, interference of magnesium sensors, etc.

Inactive Publication Date: 2008-05-07
RADIOMETER AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, divalent manganese ions are electroactive species, which can lead to unstable zero-point current in electrochemical sensors
Also, manganese may cause interference with magnesium sensors

Method used

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  • Enzyme sensor including a water-containing spacer layer
  • Enzyme sensor including a water-containing spacer layer
  • Enzyme sensor including a water-containing spacer layer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1: Effect of manganese ions on creatinine sensor measurement.

[0101] Modified from Radiometer Medical ApS, ABL of Denmark TM 735 blood analyzer, thus adapting to the above-mentioned dual sensor system. ("Exemplary sensor construction (conventional sensor)").

[0102] Various concentrations of manganese ions were added to radiometer cleaning solution S4970 samples. Manganese was added in the form of manganese(II) acetate to obtain concentrations of approximately 2 μM and 10 μM, respectively.

[0103] The stability of the creatinine sensor was checked by observing the sensor's response to a calibration solution containing each creatinine and creatine substrate.

[0104] Calibration solutions were prepared by dissolving approximately 200 μM creatinine in Radiometer Calibration Solution 1 S1720 and approximately 200 μM creatine in Radiometer Calibration Solution 2 S1730. These solutions are repeatedly introduced into the instrument and a sensor response is obt...

Embodiment 2

[0112] Example 2: Operation of the creatinine sensor.

[0113] Modified from Radiometer Medical ApS, ABL of Denmark TM 837 blood analyzer, thus adapting to the above-mentioned dual sensor system. The cleaning solution contained divalent manganese ions at a concentration of 10 μM. The sensor cleaning solution is also used to store the sensor between measurements and calibrations.

[0114] Standard operating procedures for the instrument included single-point calibrations (one calibration solution) every 4 hours and dual-point calibrations (two calibration solutions) every 8 hours. Typically, about 40 measurements are performed per day (24 hours).

[0115] When performing calibration or sample measurements, the cleaning solution is removed from the sensor and the calibration fluid or sample, respectively, is pumped into the sensor. Allow the sample / liquid to persist for 30 seconds. Afterwards, the calibrator or sample is removed by flushing with a cleaning solution, which c...

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Abstract

A method is provided for stabilising a creatinine sensor comprising creatininase as a bioactive molecule. The creatininase is stabilised by exposing it to a sufficient amount of a divalent manganese ion in a concentration of in the range of 0.01-150 [mu]M. This result may be obtained by exposing the sensor to a solution comprising the divalent manganese ion or by introducing a composition within the sensor providing sustained release of the divalent manganese ion.

Description

field of invention [0001] The present invention relates to methods of stabilizing or reactivating creatinine sensors comprising creatininase as the biologically active molecule, as well as stabilized sensors and sensor membranes comprising creatininase as the biologically active molecule. The invention also relates to apparatus for carrying out the method. Background of the invention [0002] Sensors for determining parameters of physiological samples are widely used in various fields of chemistry, biology and physiology. The presence and concentration of substances that can be degraded by biologically active molecules, such as enzymes, can be determined using a sensor comprising a suitable enzyme or enzymes. These sensors are often referred to as biosensors. Biosensors are known that apply electrochemical and spectrophotometric principles. [0003] The measurement of creatinine in physiological fluid samples, such as whole blood, serum or urine, is important for the asse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/70C12Q1/00
CPCG01N2333/978C12Q1/34
Inventor 蒂娜·克里斯滕森詹斯·奥斯特加德托马斯·杰尔
Owner RADIOMETER AS
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