Blood creatinine detection card for optical differential signal processing, preparation method and application

Abstract

The invention discloses a serum creatinine detection card for optical differential signal processing and a preparation method and application thereof.The serum creatinine detection card is composed of an upper card shell, a lower card shell, a diffusion layer, a filtering reaction layer, a separation reaction layer and a chromogenic reaction layer, the filtering reaction layer is provided with creatininase and creatine hydrolase, the separation reaction layer is provided with sarcosine oxidase and anti-interference enzyme liquid, and the chromogenic reaction layer is provided with an anti-interference enzyme liquid. Chromatogen substrates and catalase are arranged on the chromogenic reaction layer, serum is obtained through treatment and separation of the diffusion layer, the filtering reaction layer and the separation reaction layer, creatininase method step-by-step reactions are carried out on different layers respectively, enzyme liquid is arranged in a layered mode, and the substrate concentration and the enzyme liquid concentration are beneficial to full reaction of all steps; reaction by-products of all the steps cannot influence enzyme liquid of other reaction steps and the reaction process, interference is reduced, creatinine and creatine are reacted and converted to the maximum extent, the content of serum creatinine is rapidly, accurately and quantitatively detected, the accuracy and stability of the detection result are greatly improved, use is easy, and operation by professionals is not needed.

Description

technical field [0001] The invention relates to the field of quantitative detection of blood creatinine, in particular to a blood creatinine detection card for optical differential signal processing, a preparation method and an application. Background technique [0002] Creatinine (CR) is a low molecular weight nitrogenous compound produced by human metabolism. Human creatinine can be divided into serum creatinine and urinary creatinine. Serum creatinine is divided into two types: exogenous and endogenous. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. Generally, the daily production of endogenous creatinine remains almost constant. Therefore, when the total intake is stable, the concentration of serum creatinine mainly depends on the filtration function of the glomerulus, so the detection of serum creatinine is more meaningful for renal function. , can more accurately reflec...

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Problems solved by technology

[0010] However, although the optical signal differential method is also used in the prior art to eliminate the influence of endogenous creatine, the creatinine enzyme method in the prior art has a relatively large Problem, in the prior art, after the test strips in the test card diffuse the blood sample through the diffusion membrane, the blood cells are filtered out through the blood filter membrane, and then the interfering substances in the blood sample are filtered through the anti-interference membrane, and the treated serum is in the The color reaction is carried out on the reaction membrane, but in the current prior art, the 4 enzyme solutions and chromogen substrates in the 4-step reaction involved in the creatinine enzymatic method are all loaded on the reaction membrane, and all the reaction membranes are evenly loaded on the reaction membrane. Enzyme solution, therefore, the 4-step reactions involved in the creatinine enzymatic method are all completed on a reaction membrane, then, the problem is that by-products such as urea, glycine, formaldehyde and other by-products involved in the 4-step reactions involved in the creatinine enzymatic method will Enzyme activity that affects each step of the reaction, such as formaldehyde and urea, will affect the activity of creatinase and creatine hydrolase, making it ineffective, so that creatinine and creatine cannot be completely converted into creatine, resulting in inaccurate test results. Others The existence of the by-products of the steps will also affect the reaction process of the normal reaction, so that the normal required reaction process is affected, resulting in the incomplete conversion of creatinine and creatine, and the defect that the enzyme solution is all evenly arranged on the reaction membrane. The enzyme solution is evenly loaded on the reaction membrane, which leads to the low concentration of the enzyme solution in contact with the substrate in this step, so that the substrate in some positions cannot completely react with the enzyme solution, and the reaction efficiency and speed are relatively low. Low, cannot convert all the substrates of the previous step into the substrates of the subsequent reaction steps, the final result is that all the creatinine and creatine cannot be converted into hydrogen peroxide, the precursor of the color reaction, which leads to the quantitative test of creatinine There is an error, there is a deviation from the actual content of creatinine in the blood sample, the test result is inaccurate, and different enzyme solutions are uniformly loaded on the reaction membrane in a single layer, and the distribution of the enzyme solution obtained by each dipping method or spotting method is inconsistent, resulting in each test. The repeatability of the results is not good, and the deviation of the results is too large, which is a typical problem in the current optical signal differential method in the prior art

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