Blood creatinine detection card for optical differential signal processing, preparation method and application

Abstract

The invention discloses a serum creatinine detection card for optical differential signal processing and a preparation method and application thereof.The serum creatinine detection card is composed of an upper card shell, a lower card shell, a diffusion layer, a filtering reaction layer, a separation reaction layer and a chromogenic reaction layer, the filtering reaction layer is provided with creatininase and creatine hydrolase, the separation reaction layer is provided with sarcosine oxidase and anti-interference enzyme liquid, and the chromogenic reaction layer is provided with an anti-interference enzyme liquid. Chromatogen substrates and catalase are arranged on the chromogenic reaction layer, serum is obtained through treatment and separation of the diffusion layer, the filtering reaction layer and the separation reaction layer, creatininase method step-by-step reactions are carried out on different layers respectively, enzyme liquid is arranged in a layered mode, and the substrate concentration and the enzyme liquid concentration are beneficial to full reaction of all steps; reaction by-products of all the steps cannot influence enzyme liquid of other reaction steps and the reaction process, interference is reduced, creatinine and creatine are reacted and converted to the maximum extent, the content of serum creatinine is rapidly, accurately and quantitatively detected, the accuracy and stability of the detection result are greatly improved, use is easy, and operation by professionals is not needed.

Description

technical field [0001] The invention relates to the field of quantitative detection of blood creatinine, in particular to a blood creatinine detection card for optical differential signal processing, a preparation method and an application. Background technique [0002] Creatinine (CR) is a low molecular weight nitrogenous compound produced by human metabolism. Human creatinine can be divided into serum creatinine and urinary creatinine. Serum creatinine is divided into two types: exogenous and endogenous. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle tissue metabolism in the body. Generally, the daily production of endogenous creatinine remains almost constant. Therefore, when the total intake is stable, the concentration of serum creatinine mainly depends on the filtration function of the glomerulus, so the detection of serum creatinine is more meaningful for renal function. , can more accurately reflec...

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Problems solved by technology

[0010] However, although the optical signal differential method is also used in the prior art to eliminate the influence of endogenous creatine, the creatinine enzyme method in the prior art has a relatively large Problem, in the prior art, after the test strips in the test card diffuse the blood sample through the diffusion membrane, the blood cells are filtered out through the blood filter membrane, and then the interfering substances in the blood sample are filtered through the anti-interference membrane, and the treated serum is in the The color reaction is carried out on the reaction membrane, but in the current prior art, the 4 enzyme solutions and chromogen substrates in the 4-step reaction involved in the creatinine enzymatic method are all loaded on the reaction membrane, and all the reaction membranes are evenly loaded on the reaction membrane. Enzyme solution, therefore, the 4-step reactions involved in the creatinine enzymatic method are all completed on a reaction membrane, then, the problem is that by-products such as urea, glycine, formaldehyde and other by-products involved in the 4-step reactions involved in the creatinine enzymatic method will Enzyme activity that affects each step of the reaction, such as formaldehyde and urea, will affect the activity of creatinase and creatine hydrolase, making it ineffective, so that creatinine and creatine cannot be completely converted into creatine, resulting in inaccurate test results. Others Th...

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