Anion resin for bilirubin absorption and preparation method thereof

Abstract

The invention relates to anion resin for bilirubin absorption and a preparation method thereof. According to the invention, in the anion resin for bilirubin absorption, styrene is adopted as monomers, divinyl benzene is adopted as a cross-linking agent, maleic anhydride copolymer microspheres and organic small molecules are adopted as a pore-forming agent, and oil dispersible hyperoxide is adopted as an initiating agent; porous microspheres are prepared through suspension polymerization; and the anion resin with preferential bilirubin adsorption ability is obtained through quaternary ammoniation treatment, wherein the theoretical crosslinking degree of the resin is 4-20%, the average pore size of total pores is 80-120nm, and functional groups are quaternary ammonium salt groups. Accordingto the invention, the anion resin with uniform pore sizes, good permeability, good blood compatibility, high preferential adsorption of bilirubins, is obtained, and the problems in the prior art, such as poor absorption selectivity, high prices of imports, and the like, are solved.

Description

technical field

[0001] The invention belongs to blood purification adsorbents, in particular to an anionic bilirubin adsorption resin and a preparation method thereof. Background technique

[0002] Bilirubin is the metabolite of heme in aging red blood cells. Under normal circumstances, it is excreted through further metabolism after being combined with glucuronic acid in the liver. When the liver function is abnormal, the metabolism of bilirubin is disturbed, so that it accumulates in the blood (hyperbilirubinemia, Hyperbilirubinemia), which leads to bilirubin encephalopathy jaundice or nuclear jaundice. Eventually it may lead to tissue cell necrosis, trance, paralysis or death.

[0003] Phototherapy is undoubtedly the first choice for the treatment of neonatal hyperbilirubinemia in modern times. The clinical application of phototherapy in the treatment of infant hyperbilirubinemia has achieved certain curative effect. It has no obvious toxic and side effects and has been ...

Examples

Embodiment 1

[0031] Embodiment one: the preparation of anionic bilirubin adsorption resin of the present invention

[0032] (1) Synthesis of porous cross-linked microspheres: Weigh 25g of maleic anhydride copolymer microspheres of 50-100nm into a 50ml Erlenmeyer flask containing 75g of butyl acetate, that is, the mass ratio of the two is about 0.33, and ultrasonically disperse ; Accurately weigh 3g of diisopropyl peroxydicarbonate, place it in a 250ml conical flask, weigh 4g of divinylbenzene and 96g of styrene (that is, the theoretical degree of crosslinking is 4%), and add it to the conical flask , gently vibrate, after the diisopropyl peroxydicarbonate is completely dissolved, add it to a 1L three-necked flask, add the maleic anhydride copolymer microspheres / butyl acetate dispersion into the three-necked flask, shake well, ultrasonically disperse, and then add 200ml 1.5% polyvinyl alcohol solution, and finally rinse the Erlenmeyer flask with 200ml deionized water and add it to the three...

Embodiment 2

[0036] Embodiment two: the preparation of anionic bilirubin adsorption resin of the present invention

[0037] The method and steps of this embodiment are basically the same as in Example 1, except that the additions of maleic anhydride copolymer and butyl acetate are adjusted to 50g and 50g respectively, that is, the mass ratio of the two is 1, and the prepared The polymer ball parameters are as follows: specific surface area 28m 2 / g, total pore volume 0.82ml / g, total pore average pore diameter 120.0nm, large pore volume: 0.09ml / g, large pore average pore diameter 212nm, hollow average pore volume: 0.73ml / g, medium pore average pore volume 108.6 nm.

Embodiment 3

[0038] Embodiment three: the preparation of anionic bilirubin adsorption resin of the present invention

[0039] The method and steps of this example are basically the same as those of Example 1, except that the added amounts of styrene and divinylbenzene are adjusted to 20g and 80g respectively, that is, the theoretical crosslinking degree is 20%. The prepared polymer ball parameters are as follows: specific surface area 39m 2 / g, total pore volume 0.75ml / g, total pore average pore diameter 80.1nm, large pore volume: 0.06ml / g, large pore average pore diameter 175.2nm, hollow average pore volume: 0.69ml / g, medium pore average pore volume 81.8nm.

technical field

[0001] The invention belongs to blood purification adsorbents, in particular to an anionic bilirubin adsorption resin and a preparation method thereof. Background technique

[0002] Bilirubin is the metabolite of heme in aging red blood cells. Under normal circumstances, it is excreted through further metabolism after being combined with glucuronic acid in the liver. When the liver function is abnormal, the metabolism of bilirubin is disturbed, so that it accumulates in the blood (hyperbilirubinemia, Hyperbilirubinemia), which leads to bilirubin encephalopathy jaundice or nuclear jaundice. Eventually it may lead to tissue cell necrosis, trance, paralysis or death.

[0003] Phototherapy is undoubtedly the first choice for the treatment of neonatal hyperbilirubinemia in modern times. The clinical application of phototherapy in the treatment of infant hyperbilirubinemia has achieved certain curative effect. It has no obvious toxic and side effects and has been ...

Examples

Embodiment 1

[0031] Embodiment one: the preparation of anionic bilirubin adsorption resin of the present invention

[0032] (1) Synthesis of porous cross-linked microspheres: Weigh 25g of maleic anhydride copolymer microspheres of 50-100nm into a 50ml Erlenmeyer flask containing 75g of butyl acetate, that is, the mass ratio of the two is about 0.33, and ultrasonically disperse ; Accurately weigh 3g of diisopropyl peroxydicarbonate, place it in a 250ml conical flask, weigh 4g of divinylbenzene and 96g of styrene (that is, the theoretical degree of crosslinking is 4%), and add it to the conical flask , gently vibrate, after the diisopropyl peroxydicarbonate is completely dissolved, add it to a 1L three-necked flask, add the maleic anhydride copolymer microspheres / butyl acetate dispersion into the three-necked flask, shake well, ultrasonically disperse, and then add 200ml 1.5% polyvinyl alcohol solution, and finally rinse the Erlenmeyer flask with 200ml deionized water and add it to the three...

Embodiment 2

[0036] Embodiment two: the preparation of anionic bilirubin adsorption resin of the present invention

[0037] The method and steps of this embodiment are basically the same as in Example 1, except that the additions of maleic anhydride copolymer and butyl acetate are adjusted to 50g and 50g respectively, that is, the mass ratio of the two is 1, and the prepared The polymer ball parameters are as follows: specific surface area 28m 2 / g, total pore volume 0.82ml / g, total pore average pore diameter 120.0nm, large pore volume: 0.09ml / g, large pore average pore diameter 212nm, hollow average pore volume: 0.73ml / g, medium pore average pore volume 108.6 nm.

Embodiment 3

[0038] Embodiment three: the preparation of anionic bilirubin adsorption resin of the present invention

[0039] The method and steps of this example are basically the same as those of Example 1, except that the added amounts of styrene and divinylbenzene are adjusted to 20g and 80g respectively, that is, the theoretical crosslinking degree is 20%. The prepared polymer ball parameters are as follows: specific surface area 39m 2 / g, total pore volume 0.75ml / g, total pore average pore diameter 80.1nm, large pore volume: 0.06ml / g, large pore average pore diameter 175.2nm, hollow average pore volume: 0.69ml / g, medium pore average pore volume 81.8nm.