Kit for mensurating total bilirubin through chemistry oxidation process

Abstract

A kit using chemical oxidation method to analyze total bilirubin is composed of reagent A prepared from 0.5-3g / L monopotassium phosphate (MP), 16-82g / L of dibasic sodium phosphate ( DSP), 2.5-12.5g / L sodium chloride, 1-30g / L of PVP, 0.2-1g / L of complexone IV B, 5-3ml / L of quton 1000, 0.05-0.25g / L of merthiolate and pH value 7.6-8.4 of MP-DSP buffer liquid; reagent B prepared from 1-5.5g / L of DPS, 0.25-1.5g / L of MP, 12.5-65g / L of superpotassium sulfate, 4.5-22.5g / L of sodium chloride, 0.05-0.25g / L of merthiolate and pH value 6.8-7.6 of MP-DSP buffer liquid.

Description

technical field [0001] The invention relates to a kit for measuring total bilirubin in serum, plasma or urine, in particular to a kit for measuring total bilirubin by a chemical oxidation method. Background technique [0002] Total bilirubin is the general term for conjugated or unconjugated bilirubin produced by heme metabolism in the human body. Abnormally high total bilirubin is often seen in liver and gallbladder diseases. In recent years, some studies have shown that bilirubin in blood is lower than normal Often associated with coronary heart disease. Therefore, the determination of total bilirubin in human serum, plasma or urine is one of the most routine items in medical testing. [0003] There are currently three main kits for determining total bilirubin: kits for the determination of total bilirubin by the diazotization method, kits for the determination of total bilirubin by the enzymatic method, and kits for the determination of total bilirubin by the chemical ox...

AI Technical Summary

Problems solved by technology

However, using this kit to detect total bilirubin is complicated, the reagents are unstable, and the reaction is not specific. It has always been a difficult problem for medical workers. Although people have made numerous improvements for more than 100 years, there is still no Satisfying result

But it still has the following disadvantages: 1. The linear upper limit is small and cannot completely cover all pathological changes; 2. Poor anti-interference; 3. The influence of side effects is still obvious, and the response curve is not standardized and has not reached the end point; 4. The measured Accuracy, precision and sensitivity are low, especially at low values

Due to the existence of the above shortcomings, the kit for measuring total bilirubin by potassium persulfate oxidation method has not been practically applied so far.

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