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Kit for mensurating total bilirubin through chemistry oxidation process

A total bilirubin and chemical oxidation technology, which is applied in the field of kits for the determination of total bilirubin by chemical oxidation, can solve the problems of not reaching the end point, irregular reaction curve, low accuracy, precision and sensitivity, etc.

Active Publication Date: 2010-12-08
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using this kit to detect total bilirubin is complicated, the reagents are unstable, and the reaction is not specific. It has always been a difficult problem for medical workers. Although people have made numerous improvements for more than 100 years, there is still no Satisfying result
However, it still has the following disadvantages: 1. The upper limit of linearity is small and cannot completely cover all pathological changes; 2. Poor anti-interference; 3. The influence of side reactions is still obvious, the response curve is not standardized, and the end point has not been reached; 4. Measurement Accuracy, precision and sensitivity are still low, especially at low values
Due to the existence of the above shortcomings, the kit for measuring total bilirubin by potassium persulfate oxidation method has not been practically applied so far.

Method used

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  • Kit for mensurating total bilirubin through chemistry oxidation process
  • Kit for mensurating total bilirubin through chemistry oxidation process
  • Kit for mensurating total bilirubin through chemistry oxidation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Reagent 1 (R 1 )

[0075] Potassium dihydrogen phosphate (KH 2 PO 4 ) 3g

[0076] Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 82g

[0077] Sodium chloride (NaCl) 2.5g

[0078] Polyvinylpyrrolidone [(C 6 h 9 NO)n] 30g

[0079] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 0.2g

[0080] Triton X-100 (TritonX-100) 3ml

[0081] Thimerosal (C 9 h 9 o 2 HgNaS) 0.05g

[0082] h 2 Add O (distilled water, the same below) to 1000ml

[0083] Disodium hydrogen phosphate is mixed with potassium dihydrogen phosphate to adjust the pH value to 8.4.

[0084] Reagent 2 (R 2 )

[0085] Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 5.5g

[0086] Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.5g

[0087] Potassium persulfate (K 2 S 2 o 8 ) 12.5g

[0088] Sodium chloride (NaCl) 22.5g

[0089] Thimerosal (C 9 h 9 o 2 HgNaS) 0.05g

[0090] h 2 O to 1000ml

[0091] Disodium hydrogen phosphate and p...

Embodiment 2

[0102] Reagent 1 (R 1 )

[0103] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.5g

[0104] Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 16g

[0105] Sodium chloride (NaCl) 12.5g

[0106] Polyvinylpyrrolidone [(C 6 h 9 NO)n] 1g

[0107] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 1g

[0108] Triton X-100 (TritonX-100) 0.5ml

[0109] Thimerosal (C 9 h 9 o 2 HgNaS) 0.25g

[0110] h 2 O to 1000ml

[0111] Disodium hydrogen phosphate and potassium dihydrogen phosphate are mixed to adjust the pH value to 7.6.

[0112] Reagent 2 (R 2 )

[0113]Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 1g

[0114] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.25g

[0115] Potassium persulfate (K 2 S 2 o 8 ) 65g

[0116] Sodium chloride (NaCl) 4.5g

[0117] Thimerosal (C 9 h 9 o 2 HgNaS) 0.25g

[0118] h 2 O to 1000ml

[0119] Disodium hydrogen phosphate is mixed with potassium dihydrogen phosphate t...

Embodiment 3

[0122] Reagent 1 (R 1 )

[0123] Potassium dihydrogen phosphate (KH 2 PO 4 ) 2g

[0124] Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 50g

[0125] Sodium chloride (NaCl) 4g

[0126] Polyvinylpyrrolidone [(C 6 h 9 NO)n] 20g

[0127] Disodium ethylenediaminetetraacetic acid (EDTA-Na 2 ) 0.3g

[0128] Triton X-100 (TritonX-100) 1.75ml

[0129] Thimerosal (C 9 h 9 o 2 HgNaS) 0.15g

[0130] h 2 O to 1000ml

[0131] Disodium hydrogen phosphate and potassium dihydrogen phosphate are mixed to adjust the pH value to 7.8.

[0132] Reagent 2 (R 2 )

[0133] Disodium hydrogen phosphate (containing 12H 2 O)(Na 2 HPO 4 .12H 2 O) 1.6g

[0134] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.4g

[0135] Potassium persulfate (K 2 S 2 o 8 ) 40g

[0136] Sodium chloride (NaCl) 7g

[0137] Thimerosal (C 9 h 9 o 2 HgNaS) 0.15g

[0138] h 2 O to 1000ml

[0139] Disodium hydrogen phosphate and potassium dihydrogen phosphate are mixed to...

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Abstract

A kit using chemical oxidation method to analyze total bilirubin is composed of reagent A prepared from 0.5-3g / L monopotassium phosphate (MP), 16-82g / L of dibasic sodium phosphate ( DSP), 2.5-12.5g / L sodium chloride, 1-30g / L of PVP, 0.2-1g / L of complexone IV B, 5-3ml / L of quton 1000, 0.05-0.25g / L of merthiolate and pH value 7.6-8.4 of MP-DSP buffer liquid; reagent B prepared from 1-5.5g / L of DPS,0.25-1.5g / L of MP, 12.5-65g / L of superpotassium sulfate, 4.5-22.5g / L of sodium chloride, 0.05-0.25g / L of merthiolate and pH value 6.8-7.6 of MP-DSP buffer liquid.

Description

technical field [0001] The invention relates to a kit for measuring total bilirubin in serum, plasma or urine, in particular to a kit for measuring total bilirubin by a chemical oxidation method. Background technique [0002] Total bilirubin is the general term for conjugated or unconjugated bilirubin produced by heme metabolism in the human body. Abnormally high total bilirubin is often seen in liver and gallbladder diseases. In recent years, some studies have shown that bilirubin in blood is lower than normal Often associated with coronary heart disease. Therefore, the determination of total bilirubin in human serum, plasma or urine is one of the most routine items in medical testing. [0003] There are currently three main kits for determining total bilirubin: kits for the determination of total bilirubin by the diazotization method, kits for the determination of total bilirubin by the enzymatic method, and kits for the determination of total bilirubin by the chemical ox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/72G01N21/25
Inventor 邹炳德
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD