31 results about "Hemoglobin G Szuhu" patented technology

A system for determining a biologic constituent including hematocrit transcutaneously, noninvasively and continuously. A finger clip assembly includes including at least a pair of emitters and a photodiode in appropriate alignment to enable operation in either a transmissive mode or a reflectance mode. At least one predetermined wavelength of light is passed onto or through body tissues such as a finger, earlobe, or scalp, etc. and attenuation of light at that wavelength is detected. Likewise, the change in blood flow is determined by various techniques including optical, pressure, piezo and strain gage methods. Mathematical manipulation of the detected values compensates for the effects of body tissue and fluid and determines the hematocrit value. If an additional wavelength of light is used which attenuates light substantially differently by oxyhemoglobin and reduced hemoglobin, then the blood oxygen saturation value, independent of hematocrit may be determined. Further, if an additional wavelength of light is used which greatly attenuates light due to bilirubin (440 nm) or glucose (1060 nm), then the bilirubin or glucose value may also be determined. Also how to determine the hematocrit with a two step DC analysis technique is provided. Then a pulse wave is not required, so this method may be utilized in states of low blood pressure or low blood flow.

An oxygen concentration measurement system for blood hemoglobin comprises a multiple-wavelength low-coherence optical light source that is coupled by single mode fibers through a splitter and combiner and focused on both a target tissue sample and a reference mirror. Reflections from both the reference mirror and from the depths of the target tissue sample are carried back and mixed to produce interference fringes in the splitter and combiner. The reference mirror is set such that the distance traversed in the reference path is the same as the distance traversed into and back from the target tissue sample at some depth in the sample that will provide light attenuation information that is dependent on the oxygen in blood hemoglobin in the target tissue sample. Two wavelengths of light are used to obtain concentrations. The method can be used to measure total hemoglobin concentration [Hb.sub.deoxy +Hb.sub.oxy ] or total blood volume in tissue and in conjunction with oxygen saturation measurements from pulse oximetry can be used to absolutely quantify oxyhemoglobin [HbO.sub.2 ] in tissue. The apparatus and method provide a general means for absolute quantitation of an absorber dispersed in a highly scattering medium.

The invention discloses a biological tissue blood flow, blood oxygen and blood volume multi-parameter detection method and device. According to the method, two laser beams, differing in wavelength, simultaneously irradiate a biological tissue; and multiple frames of biological tissue colored images, which are illuminated by the laser beams, are continuously acquired by a photoelectric imaging system. The light intensity values of a red channel and a green channel on each pixel of the images are obtained for respectively constituting a red channel image and a green channel image. By virtue of a laser speckle blood flow imaging data analysis method and a dual-wavelength spectroscopic data analysis method, the red channel image and the green channel image are processed, so that two-dimensional spatial distribution and dynamic variation information thereof of a plurality of physiological parameters including blood flow, oxyhemoglobin concentration and reduced hemoglobin concentration of the corresponding biological tissue are obtained. The method and the device disclosed by the invention solve the shortcoming that a multi-wavelength time-sharing imaging based multi-parameter blood flow imaging system has difficulty in achieving the synchronization of accurate time and also avoid the shortcoming that a multi-camera shooting based multi-parameter blood flow imaging system is high in cost due to the use of a plurality of cameras.

The invention belongs to medical equipment, relates to a flow path structure of a glycated hemoglobin analyzer, in particular to a backwashing flow path structure of a glycated hemoglobin analyzer. The backwashing flow path system of the glycated hemoglobin analyzer comprises a peristaltic pump positioned on a liquid feeding pipeline, wherein a liquid discharging hole of the peristaltic pump is introduced into a waste liquid bottle through a liquid discharging pipe after sequentially passing through a first electromagnetic valve, a cation exchange resin chromatographic column, a cuvette cell and a second electromagnetic valve; and a third electromagnetic valve is connected between the liquid discharging hole of the peristaltic pump and an outlet of the cuvette cell, and a branching pipeline is arranged in front of the inlet end of the cation exchange resin chromatographic column and is connected with the liquid discharging pipeline through a fourth electromagnetic pipeline. The invention has the advantages of being capable of avoiding aggregation and blockage of floccules in a detected sample at the chromatographic column and a filter screen of the glycated hemoglobin analyzer, prolonging the service life of the chromatographic column, improving reliability of a detection process, and being convenient for operation and maintenance and low in cost.

The invention discloses collection liquid for protecting and stabilizing hemoglobin in manure and application of the collection liquid. The collection liquid comprises 25-45 g/L of chelating agent, 0.2-1 g/L of preservative, 1-10 g/L of sealing agent, 6-12 g/L of buffering agent and 25-30 g/L of polyethylene glycol, wherein the chelating agent is a mixture of two of HIDS, EDTA and sodium tripolyphosphate. After fresh manure and the collection liquid are mixed well according to a proportion of 50 mg:2 ml, the collection liquid can be stored for 7 days at normal temperature, one month at 2-8 DEG C and six months at minus 20 DEG C. The collection liquid can further stabilize hemoglobin and prolong storage time of hemoglobin in manure in protection liquid.

The invention discloses a cigarette filter containing a material selectively decreasing biological harm of carbon monoxide (CO) in smoke and a preparation method of the cigarette filter, belonging to the technical field of the cigarette filter. The cigarette filter provided by the invention is characterized in that a nanometer mesoporous material of self-assembling immobilized hemoglobin of amphiphilic diblock copolymer of polyethylene glycol-polycaprolactone is added into a tow material, wherein the nanometer mesoporous material accounts for 10-25% of the total weight of the tow material. The preparation method of the cigarette filter provided by the invention comprises the following steps: [1] immobilizing hemoglobin in the amphiphilic diblock copolymer of polyethylene glycol-polycaprolactone with the average molecular weight of 8000, 6000 or 4000; and [2] adding the HINH material (nanometer mesoporous material) into the tow material of the cigarette filter. The cigarette filter provided by the invention has a function of selectively decreasing the biological harm of CO in the smoke. An immobilization condition of the PEG-PCL (polyethylene glycol-polycaprolactone) immobilized hemoglobin is mild, the activity of the hemoglobin is guaranteed, the storage period is long and the fragrance of flue-cured type cigarettes is not damaged.

The invention discloses a method for measuring the content of free hemoglobin in a blood bag by a multiple-position modulated light source. The method comprises the following steps: modulating a light source by a modulation device, allowing the light exit port of the light source and the entrance slit of a spectrum receiving device to cling to the blood bag, controlling the light source to move to multiple positions by a displacement platform in order to transmit a blood sample, and acquiring the transmission spectrum by the spectrum receiving device; transforming the time sequence of every wavelength in the acquired transmission spectrum into a frequency domain, constructing a transmission spectrum in the frequency domain based on the fundamental wave component of every wavelength, carrying out normalization processing, comparing the obtained result with existing chemical analysis results, and establishing a mathematical model; and acquiring the transmission spectrum in the frequency domain of an unknown blood sample through using above steps, normalizing the obtained transmission spectrum, and substituting the normalization result into the mathematical model in order to obtain the content of the free hemoglobin. The method eliminates the influences brought by spectral background noises and scattering, increases the information content of all components in the blood, and improves the precision of the free hemoglobin content analysis.

The invention discloses a method for measuring the content of free hemoglobin by double-optical-path transmittance and fluorescence spectra. The method includes transmitting a blood sample in a blood bag by a transmittance light source, and exciting the blood sample in the blood bag by the fluorescence excitation light source, wherein light sources include the transmittance light source and the fluorescence excitation light source; controlling the light sources to move by a displacement platform on the premise that the light outlets of the light sources are coaxial with a entrance slit of a spectrum receiving device, and collecting the transmittance spectra and the fluorescence spectra by the spectrum receiving device; solving the logarithm of the light intensity ratio of the double-optical-path transmittance spectra under each wavelength to acquire absorption spectra, combining and normalizing the absorption spectra and the two fluorescence spectra, combining with chemical testing data, and creating a mathematical model; collecting transmittance spectra and fluorescence spectra of an unknown blood sample under two optical paths, and after normalization, inputting the absorption spectra, acquired by solving the logarithm of the ratio of the two transmittance spectra, and the two fluorescence spectra into the mathematical model for calculation to acquire the content of the free hemoglobin.

In one aspect, the inventive concepts disclosed herein are directed to a chromatographic assay device for detecting the presence of free hemoglobin in a whole blood sample. The device comprising a chromatographic detection pad with a sample application site and a detection side. The chromatographic detection pad defines a path for capillary fluid flow. The chromatographic detection pad has a pore size. The sample application site on the chromatographic detection pad is for application of a portion of the whole blood sample. The detection site on the chromatographic detection pad is spaced apart from the application site and is downstream of the sample application site. The chromatographic detection pad is devoid of a compound located downstream of the application site that is reactive to the whole blood sample.

Measurement system and methods for measuring oxygenated hemoglobin saturation level are provided. Light is transmitted to test blood and a reference mirror. The reference mirror provides a first reflected light beam, and backscattered light from different depths of the test blood generates a second reflected light beam. An interfered light signal is generated by light interference of the first and second reflected light beams. According to the interfered light signal, a first light decay constant for a first light wavelength range and a second light decay constant for a second light wavelength range are obtained according to the interfered light signal. A decay ratio of the first light decay constant to the second light decay constant is obtained. Oxygenated hemoglobin saturation level of the test blood is obtained according to the decay ratio.

The invention discloses a method for measuring free hemoglobin by a double-optical-path-length and multi-position modulation fluorescent excitation light source. The method comprises the following steps that a light outlet opening of the fluorescent excitation light source and an incident slit of a spectrum receiving device are tightly attached to a blood bag; a modulation device modulates the fluorescent excitation light source; the fluorescent excitation light source excites the blood sample; the spectrum receiving device collects the fluorescence spectrum; a displacement platform controls the fluorescent excitation light source to move to different positions; the optical path length measurement is changed; the fluorescence spectrum collected in a plurality of positions under the conditions of the two optical path lengths is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures; the fluorescence spectrum in a plurality of frequency domains is subjected to normalization processing; the chemical inspection data is combined; a mathematical model is built; the fluorescence spectrum of an unknown blood sample in the plurality of positions under the condition of the two optical path lengths is collected; after the fluorescence spectrum is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures, normalization processing is performed; the data is substituted into the mathematical model to obtain the content of the free hemoglobin.

The invention discloses a method for measuring free hemoglobin by double-optical-path modulating transmittance and fluorescence excitation light sources. The method includes modulating the transmittance light source and the fluorescence excitation light source by a modulating device, and transmitting and exciting blood in a blood bag by the transmittance light source and the fluorescence excitation light source respectively; controlling the light sources to move by a displacement platform, and collecting transmittance spectra and fluorescence spectra by a spectrum receiving device; transforming time sequence of each wavelength of the transmittance spectra and the fluorescence spectra into a frequency domain, creating the intra-frequency-domain transmittance spectra and the intra-frequency-domain fluorescence spectra by fundamental wave component of each wavelength, solving the logarithms of light intensity ratios of the two created intra-frequency-domain transmittance spectra under each wavelength to acquire absorption spectra, normalizing the absorption spectra and the two intra-frequency-domain fluorescence spectra, comparing with existing chemical analysis results and creating a mathematical model; collecting absorption spectra and intra-frequency-domain fluorescence spectra of blood in an unknown blood bag according to the same method, and inputting the absorption spectra and the intra-frequency-domain fluorescence spectra into the mathematical model after normalization to acquire the content of the free hemoglobin.

The invention provides a preparation method of an occult blood critical value quality control material, which comprises the following steps: (1) quality control material container sealing: filling a centrifugal tube with a specification of 0.5 ml by using 0.5-2.0 percent bovine serum albumin, putting into a refrigerator with a temperature of 4 DEG C, sealing for 24 hours, then abandoning liquid in the centrifugal tube and putting the centrifugal tube into a biological safety cabinet to naturally air for standby; (2) critical quality control material preparation: taking a blood routine test specimen of a normal healthy physical examinee, dissolving blood with distilled water according to hemoglobin test results, diluting into 1-2 mg/L, adding a protein stabilizer as well as sodium azide till the final concentration is 2.0-7.0 g/L and 0.2-2.0 g/L respectively and sub-packaging into 0.5 ml sealed centrifugal tube, wherein a solution in each tube is 50-500 ul; and (3) quality control material preservation: preserving the sub-packaged critical value quality control material at a temperature of 4-20 DEG C. The preparation method has the beneficial effects that the preparation is simple, whether a corresponding reagent deteriorates during the processes of circulation, preservation and use or not can be monitored, and the period of validity is long.

The invention discloses a method for measuring free hemoglobin in a blood bag by multi-position modulation of a fluorescence excitation light source. According to the method, the fluorescence excitation light source is modulated by a modulation device, a light outlet of the fluorescence excitation light source and an entrance slit of a spectrum receiving device cling to a packaging bag, the fluorescence excitation light source excites the free hemoglobin to generate fluorescence, a displacement platform controls the fluorescence excitation light source to move to a plurality of positions, fluorescence spectrums are acquired by the spectrum receiving device, the time sequence of each wavelength in the fluorescence spectrums acquired at a plurality of positions is transformed to a frequency domain, fluorescence spectrums in the frequency domains are constructed by the aid of fundamental components with various wavelengths, the fluorescence spectrums are normalized and then compared with existing chemical analysis results, a mathematical model is built, fluorescence spectrums in frequency domains at a plurality of positions of a blood sample in an unknown blood bag are acquired by the same method and substituted into the mathematical model after normalization to obtain the content of the free hemoglobin.