Method for measuring free hemoglobin by double-optical-path modulating transmittance and fluorescence excitation light sources

Abstract

The invention discloses a method for measuring free hemoglobin by double-optical-path modulating transmittance and fluorescence excitation light sources. The method includes modulating the transmittance light source and the fluorescence excitation light source by a modulating device, and transmitting and exciting blood in a blood bag by the transmittance light source and the fluorescence excitation light source respectively; controlling the light sources to move by a displacement platform, and collecting transmittance spectra and fluorescence spectra by a spectrum receiving device; transforming time sequence of each wavelength of the transmittance spectra and the fluorescence spectra into a frequency domain, creating the intra-frequency-domain transmittance spectra and the intra-frequency-domain fluorescence spectra by fundamental wave component of each wavelength, solving the logarithms of light intensity ratios of the two created intra-frequency-domain transmittance spectra under each wavelength to acquire absorption spectra, normalizing the absorption spectra and the two intra-frequency-domain fluorescence spectra, comparing with existing chemical analysis results and creating a mathematical model; collecting absorption spectra and intra-frequency-domain fluorescence spectra of blood in an unknown blood bag according to the same method, and inputting the absorption spectra and the intra-frequency-domain fluorescence spectra into the mathematical model after normalization to acquire the content of the free hemoglobin.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring free hemoglobin by modulating transmission and fluorescence excitation light sources with dual optical paths. Background technique [0002] In the prior art, the relatively mature method is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and non-polluting characteristics, it is also possible to detect the content of free hemoglobin in blood bags. [0003] In transmission spectrum detection, according to the Lambert-Beer law: the incident light intensity I of each wavelength is measured separately 0 and the outgoing light intensity I, the absorbance A of each wavelength is calculated by the formula (1). ...

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Problems solved by technology

[0013] However, it is difficult to find exactly the same neutral attenuator in different occasions, and it is difficult to ensure that the position of the sample and the neutral attenuator are consistent, which increases the difficulty of measurement and makes it difficult to ensure measurement accuracy.

[0014] In view of the complexity of blood components, the pure transmission spectrum obtains the information of whole blood, which is poor in pertinence. In order to further improve the measurement accuracy of free hemoglobin, combined with the strong pertinence of fluorescence, it is limited by the excitation light intensity and optical path of fluorescence. Due to the influence of the length and the concentration of the measured components, the fluorescence will have a serious self-absorption problem, resulting in a strong nonlinearity in the obtained fluorescence spectrum, which cannot well reflect the characteristics of the measured substance. At the same time, the traditional fluorescence spectrum cannot fully Eliminate the effects of spectral background noise

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