Method for measuring free hemoglobin by double-optical-path-length and multi-position modulation fluorescent excitation light source

Abstract

The invention discloses a method for measuring free hemoglobin by a double-optical-path-length and multi-position modulation fluorescent excitation light source. The method comprises the following steps that a light outlet opening of the fluorescent excitation light source and an incident slit of a spectrum receiving device are tightly attached to a blood bag; a modulation device modulates the fluorescent excitation light source; the fluorescent excitation light source excites the blood sample; the spectrum receiving device collects the fluorescence spectrum; a displacement platform controls the fluorescent excitation light source to move to different positions; the optical path length measurement is changed; the fluorescence spectrum collected in a plurality of positions under the conditions of the two optical path lengths is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures; the fluorescence spectrum in a plurality of frequency domains is subjected to normalization processing; the chemical inspection data is combined; a mathematical model is built; the fluorescence spectrum of an unknown blood sample in the plurality of positions under the condition of the two optical path lengths is collected; after the fluorescence spectrum is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures, normalization processing is performed; the data is substituted into the mathematical model to obtain the content of the free hemoglobin.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring free hemoglobin with dual optical paths and multi-position modulation fluorescence excitation light sources. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, Non-contact and pollution-free requirements. [0003] The study found that the measurement of fluorescence spectroscopy is also possible to detect the content of free hemoglobin in blood bags due to its non-contact, non-polluting, and highly targeted characteristics. [0004] However, due to the influence of incident light intensity, optical path le...

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Problems solved by technology

[0004] However, due to the influence of incident light intensity, optical path length and measured target concentration, fluorescence has serious self-absorption problems, as well as blood scattering, which will lead to spectral nonlinearity, and traditional fluorescence spectroscopy cannot completely eliminate spectral background In response to this problem, this method proposes a dual optical path and multi-position fluorescence spectroscopy method to detect the content of free hemoglobin in blood bags

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