Method for measuring free hemoglobin by double-optical-path-length and multi-position modulation fluorescent excitation light source

A fluorescence excitation and hemoglobin technology, applied in fluorescence/phosphorescence, measurement devices, material excitation analysis, etc., can solve the problems of inability to completely eliminate the influence of spectral background noise, fluorescence self-absorption, spectral nonlinearity, etc., to achieve inhibition of blood scattering, Measure targeted, informative effects

Inactive Publication Date: 2017-11-24
TIANJIN UNIV
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  • Application Information

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Problems solved by technology

[0004] However, due to the influence of incident light intensity, optical path length and measured target concentration, fluorescence has serious self-absorption problems, as well as blood scattering, which will lead to spectral nonlinearity, and traditional fluorescence spectroscopy cannot completely eliminate spectral background In response to this problem, this method proposes a dual optical path and multi-position fluorescence spectroscopy method to detect the content of free hemoglobin in blood bags

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  • Method for measuring free hemoglobin by double-optical-path-length and multi-position modulation fluorescent excitation light source
  • Method for measuring free hemoglobin by double-optical-path-length and multi-position modulation fluorescent excitation light source
  • Method for measuring free hemoglobin by double-optical-path-length and multi-position modulation fluorescent excitation light source

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Embodiment 1

[0060] The embodiment of the present invention provides a method for measuring free hemoglobin with a dual optical path and multi-position modulated fluorescence excitation light source. The devices used are such as figure 2 As shown, it includes: a fluorescent excitation light source 3 , a blood bag 5 , a displacement platform 6 , a spectrum receiving device 7 and a modulating device 9 .

[0061] Wherein, ensure that the light exit port of the fluorescence excitation light source 3 and the incident slit of the spectrum receiving device 7 are close to the blood bag 5, the modulation device 9 modulates the fluorescence excitation light source 3 to make it emit a square wave light signal, and the fluorescence excitation light source 3 at position a Under the two optical paths: position a (corresponding to the first optical path 1) and position a' (corresponding to the second optical path 2) excite the blood sample in the blood bag 5, and the fluorescence spectrum is collected by...

Embodiment 2

[0070] The difference between the embodiment of the present invention and embodiment 1 is only that the fluorescence excitation light source 3 and the movement mode of the spectrum receiving device 7 are different, see the following description for details:

[0071] see image 3 To ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the spectrum receiving device 7 are close to the blood bag 5, the modulation device 9 modulates the fluorescence excitation light source 3 to make it emit a square wave light signal, and the fluorescence excitation light source 3 controls the blood bag 5 The blood sample is excited, and the spectrum receiving device 7 collects the fluorescence spectrum at the position a under the double optical path: position a and position a'. Control the spectrum receiving device 7 to move to position b through the displacement platform 6, and collect the fluorescence spectrum under the double optical path at positi...

Embodiment 3

[0076] During specific implementation, due to the limitation of the space structure, it may occur that the fluorescence excitation light source 3 and the spectrum receiving device 7 cannot be close to the blood bag 5. At this time, an optical fiber can be respectively arranged at the fluorescence excitation light source 3 and the spectrum receiving device 7 , as the incident fiber 4 and the exit fiber 8 .

[0077] see Figure 4 , the modulation device 9 modulates the fluorescence excitation light source 3 to make it emit a square wave light signal, the fluorescence excitation light source 3 excites the blood sample in the blood bag 5 through the incident optical fiber 4, the fluorescence spectrum is collected by the spectrum receiving device 7 through the exit optical fiber 8, and the incident optical fiber 4 and the outgoing optical fiber 8 are respectively close to the blood bag 5, the incident optical fiber 4 is at the position a, and the fluorescence excitation light sourc...

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Abstract

The invention discloses a method for measuring free hemoglobin by a double-optical-path-length and multi-position modulation fluorescent excitation light source. The method comprises the following steps that a light outlet opening of the fluorescent excitation light source and an incident slit of a spectrum receiving device are tightly attached to a blood bag; a modulation device modulates the fluorescent excitation light source; the fluorescent excitation light source excites the blood sample; the spectrum receiving device collects the fluorescence spectrum; a displacement platform controls the fluorescent excitation light source to move to different positions; the optical path length measurement is changed; the fluorescence spectrum collected in a plurality of positions under the conditions of the two optical path lengths is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures; the fluorescence spectrum in a plurality of frequency domains is subjected to normalization processing; the chemical inspection data is combined; a mathematical model is built; the fluorescence spectrum of an unknown blood sample in the plurality of positions under the condition of the two optical path lengths is collected; after the fluorescence spectrum is converted into the fluorescence spectrum in the frequency domains of the frequency domain structures, normalization processing is performed; the data is substituted into the mathematical model to obtain the content of the free hemoglobin.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring free hemoglobin with dual optical paths and multi-position modulation fluorescence excitation light sources. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, Non-contact and pollution-free requirements. [0003] The study found that the measurement of fluorescence spectroscopy is also possible to detect the content of free hemoglobin in blood bags due to its non-contact, non-polluting, and highly targeted characteristics. [0004] However, due to the influence of incident light intensity, optical path le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 林凌王艳军张梦秋罗永顺甄建芸王玉宇李刚
Owner TIANJIN UNIV
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