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Method for measuring free hemoglobin by double-optical-path transmission and fluorescence spectrums at multiple positions

A technique of fluorescence spectroscopy and hemoglobin, which is applied in the field of free hemoglobin measurement with dual optical path transmission and fluorescence spectroscopy at multiple positions, which can solve the problem of difficulty in ensuring that the sample is in the same position as the neutral attenuator, difficult to find a neutral attenuator, and not very good. It can eliminate the influence of the change of the transmitted light source and the blood bag, eliminate the influence and improve the accuracy.

Inactive Publication Date: 2017-12-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] But it is difficult to find exactly the same neutral attenuator in different occasions, and it is difficult to ensure that the position of the sample and the neutral attenuator are consistent
In view of the complexity of blood components, the pure transmission spectrum obtains the information of whole blood, which is poorly targeted and has a certain amount of scattering. The influence of excitation light intensity, optical path length and the concentration of the measured components will lead to serious self-absorption problems in fluorescence, and the strong scattering of blood will cause the obtained fluorescence spectrum to have strong nonlinearity, so it is not very good Responses to the characteristics of the measured substance

Method used

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  • Method for measuring free hemoglobin by double-optical-path transmission and fluorescence spectrums at multiple positions
  • Method for measuring free hemoglobin by double-optical-path transmission and fluorescence spectrums at multiple positions
  • Method for measuring free hemoglobin by double-optical-path transmission and fluorescence spectrums at multiple positions

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Embodiment 1

[0073] The embodiment of the present invention provides a method for measuring free hemoglobin with dual optical path transmission and fluorescence spectroscopy at multiple positions, and the devices used are such as figure 2 As shown, it includes: a light source 3 , a blood bag 5 , a displacement platform 6 and a spectrum receiving device 7 .

[0074] Wherein, ensure that the light outlet of the light source 3 and the incident slit of the spectrum receiving device 7 are close to the blood bag 5, and the light source 3 is under the two optical paths at position a: position a (corresponding to the first optical path 1) and position a' (corresponding to the second optical path 2) transmits and excites the blood in the blood bag 5, and the transmission spectrum and fluorescence spectrum are collected by the spectrum receiving device 7; then the light source 3 is controlled to move to the position b by the displacement platform 6, at position b Under the two optical paths at posi...

Embodiment 2

[0083] The difference between the embodiment of the present invention and embodiment 1 is only that the light source 3 and the movement mode of the spectrum receiving device 7 are different, see the following description for details:

[0084] see image 3 , ensure that the light outlet of the light source 3 and the incident slit of the spectrum receiving device 7 are close to the blood bag 5, the light source 3 transmits and excites the blood in the blood bag 5, and the spectrum receiving device 7 collects the double optical path at position a The following are: the transmission spectrum and the fluorescence spectrum of position a and position a'. The spectrum receiving device 7 is controlled to move to the position b through the displacement platform 6, and the transmission spectrum and the fluorescence spectrum of the position b and the position b' are collected under the double optical path at the acquisition position b; the spectrum receiving device 7 is controlled to move...

Embodiment 3

[0089] During specific implementation, due to the limitation of the space structure, it may occur that the light source 3 and the spectrum receiving device 7 cannot be close to the blood bag 5. At this time, an optical fiber can be respectively arranged at the light source 3 and the spectrum receiving device 7 as the incident optical fiber 4 and outgoing fiber 8.

[0090] see Figure 4, the light source 3 transmits and excites the blood in the blood bag 5 through the incident optical fiber 4, the transmission spectrum and the fluorescence spectrum are collected by the spectrum receiving device 7 through the outgoing optical fiber 8, the incident optical fiber 4 and the outgoing optical fiber 8 are respectively close to the blood bag 5, and the incident The optical fiber 4 is at position a, and the light source 3 transmits and excites the blood through the incident optical fiber 4 under the double optical path at this position, that is, position a and position a', and the trans...

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Abstract

The invention discloses a method for measuring free hemoglobin by double-optical-path transmission and fluorescence spectrums at multiple positions. According to the method, a displacement platform controls a light source to move to a plurality of positions, blood is transmitted and excited, a double-optical-path transmission and fluorescence spectrum at each position is acquired by a spectrum receiving device, transmission spectrum ratios under two optical paths at each position are subjected to logarithm to obtain a blood absorption spectrum at the position, absorption spectrums at a plurality of positions and double-optical-path fluorescence spectrums at plurality of positions are normalized together, a mathematical model is built according to chemical examination data, double-optical-path transmission spectrums and fluorescence spectrums at a plurality of positions of unknown blood are acquired, transmission spectrum ratios under two light paths at each position are subjected to logarithm to obtain a blood absorption spectrum at the position, and absorption spectrums at a plurality of positions and double-optical-path fluorescence spectrums at plurality of positions are normalized and substituted into the mathematical model to obtain the content of the free hemoglobin by calculation.

Description

technical field [0001] The invention relates to the field of spectroscopic blood concentration analysis stoichiometry, in particular to a method for measuring free hemoglobin with dual optical path transmission and fluorescence spectroscopy at multiple positions. Background technique [0002] In the existing technology, the relatively mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the way of chemical testing cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and non-polluting characteristics, it is also possible to detect the content of free hemoglobin in blood bags. [0003] In spectral detection, according to the Lambert-Beer law: the incident light intensity I of each wavelength is measured separately 0 and the outgoing light intensity I, the absorbance A of each wavelength is calculated by the formula (1). ∈ is the abso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31
CPCG01N21/6402G01N21/31G01N21/64G01N2021/6417
Inventor 林凌张盛昭甄建芸王玉宇王艳军张梦秋李刚
Owner TIANJIN UNIV
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