Method for measuring content of free hemoglobin by double-optical-path transmittance and fluorescence spectra

Abstract

The invention discloses a method for measuring the content of free hemoglobin by double-optical-path transmittance and fluorescence spectra. The method includes transmitting a blood sample in a blood bag by a transmittance light source, and exciting the blood sample in the blood bag by the fluorescence excitation light source, wherein light sources include the transmittance light source and the fluorescence excitation light source; controlling the light sources to move by a displacement platform on the premise that the light outlets of the light sources are coaxial with a entrance slit of a spectrum receiving device, and collecting the transmittance spectra and the fluorescence spectra by the spectrum receiving device; solving the logarithm of the light intensity ratio of the double-optical-path transmittance spectra under each wavelength to acquire absorption spectra, combining and normalizing the absorption spectra and the two fluorescence spectra, combining with chemical testing data, and creating a mathematical model; collecting transmittance spectra and fluorescence spectra of an unknown blood sample under two optical paths, and after normalization, inputting the absorption spectra, acquired by solving the logarithm of the ratio of the two transmittance spectra, and the two fluorescence spectra into the mathematical model for calculation to acquire the content of the free hemoglobin.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of complex spectral solutions, in particular to a method for measuring the content of free hemoglobin with dual optical path transmission and fluorescence spectroscopy. Background technique [0002] In the prior art, the relatively mature method is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and non-polluting characteristics, it is also possible to detect the content of free hemoglobin in blood bags. [0003] In spectral detection, according to the Lambert-Beer law: the incident light intensity I of each wavelength is measured separately 0 and the outgoing light intensity I, the absorbance A of each wavelength is calculated by the formula (1). ∈ is the absorption coef...

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Problems solved by technology

[0013] However, it is difficult to find exactly the same neutral attenuator in different occasions, and it is difficult to ensure that the position of the sample and the neutral attenuator are consistent, which increases the difficulty of measurement and makes it difficult to ensure measurement accuracy.

[0014] In view of the complexity of blood components, the pure transmission spectrum obtains the information of whole blood, which is poor in pertinence. In order to further improve the measurement accuracy of free hemoglobin, combined with the strong pertinence of fluorescence, it is limited by the excitation light intensity and optical path of fluorescence. Due to the influence of the length and the concentration of the measured components, the fluorescence will have a serious self-absorption problem, resulting in a strong nonlinearity in the obtained fluorescence spectrum, which cannot well reflect the characteristics of the measured substance.

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