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Method for measuring content of free hemoglobin in blood bag by double-light-path fluorescent light intensity

A technology of hemoglobin and fluorescent light, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems that cannot meet the requirements of fast, simple, non-contact, and pollution-free, and achieve pollution-free high-precision measurement, The effect of strong measurement pertinence and simple measurement of fluorescent light intensity

Inactive Publication Date: 2017-09-26
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Convenience, non-contact, and pollution-free requirements, in response to this problem, the present invention proposes a dual-path fluorescence light intensity method to detect the content of free hemoglobin in blood bags

Method used

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  • Method for measuring content of free hemoglobin in blood bag by double-light-path fluorescent light intensity
  • Method for measuring content of free hemoglobin in blood bag by double-light-path fluorescent light intensity
  • Method for measuring content of free hemoglobin in blood bag by double-light-path fluorescent light intensity

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Embodiment 1

[0053] The embodiment of the present invention provides a method for measuring free hemoglobin content in a blood bag with dual optical path fluorescence light intensity. The devices used are such as figure 1 As shown, it includes: a fluorescent excitation light source 3 , a blood bag 5 , a displacement platform 6 and a light intensity receiving device 7 .

[0054] Wherein, ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the light intensity receiving device 7 are close to the blood bag 5 and coaxial, and the fluorescence excitation light source 3 faces the blood bag 5 at the first position a (corresponding to the first optical path 1). The blood sample inside is excited, and the fluorescent light intensity is received by the light intensity receiving device 7 . Subsequently, the displacement platform 6 controls the fluorescence excitation light source to move to the second position b (corresponding to the second optical path ...

Embodiment 2

[0061] The difference between the embodiment of the present invention and the embodiment 1 is only that the fluorescence excitation light source 3 and the movement mode of the light intensity receiving device 7 are different, see the following description for details:

[0062] see figure 2 To ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the light intensity receiving device 7 are close to the blood bag 5 and coaxial, the fluorescence excitation light source 3 excites the blood sample in the blood bag 5, and the light intensity receiving device 7 A position a receives fluorescent light intensity. Then, the displacement platform 6 is used to control the light intensity receiving device 7 to move to the second position b under the premise of ensuring that the light outlet of the fluorescence excitation light source 3 is coaxial with the incident slit of the light intensity receiving device 7, and receive the fluorescence at t...

Embodiment 3

[0067] The difference between the embodiment of the present invention and embodiment 1 is only that the fluorescent excitation light source 3 and the moving direction of the light intensity receiving device 7 are different, see the following description for details:

[0068] see image 3 , ensure that the fluorescence excitation light source 3 and the light intensity receiving device 7 are close to the blood bag 5 and ensure that the light outlet of the fluorescence excitation light source 3 and the incident slit of the light intensity receiving device 7 are coaxial, and the fluorescence excitation light source 3 is paired at the first position a The blood sample in the blood bag 5 is excited, and the fluorescent light intensity is received by the light intensity receiving device 7 at the first position a', and then the displacement platform 6 is used to ensure that the light output port of the fluorescence excitation light source 3 and the light intensity receiving device 7 ar...

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Abstract

The invention discloses a method for measuring the content of free hemoglobin in a blood bag by double-light-path fluorescent light intensity. The method comprises the following steps of making a light outlet of a fluorescent excitation light source and an entrance slit of a light intensity receiving device cling to the blood bag and be further coaxial, exciting a blood sample by the fluorescent excitation light source, and receiving fluorescent light intensity by the light intensity receiving device; controlling the fluorescent excitation light source to move by a displacement platform on the premise of guaranteeing the light outlet of the fluorescent excitation light source and the entrance silt of the light intensity receiving device to be coaxial, and receiving the fluorescent light intensity by the light intensity receiving device; subjecting two pieces of the collected fluorescent light intensity to normalization treatment, and establishing a mathematic model by combining with the data of a chemical examination; collecting the fluorescent light intensity of an unknown blood sample at two positions, subjecting to normalization, and substituting into the mathematic model, so as to obtain the content of the free hemoglobin. By using the method, the non-destructive examination of the free hemoglobin is realized; the method is high-efficiency and pollution-free, and is high in measurement pertinence; the analysis accuracy of the content of the free hemoglobin is obviously improved; further, the measurement by the fluorescent light intensity is simple and convenient.

Description

technical field [0001] The invention relates to the field of stoichiometry for concentration analysis of complex solutions with light intensity, in particular to a method for measuring the content of free hemoglobin in a blood bag with double optical path fluorescence light intensity. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Convenience, non-contact, and pollution-free requirements, in view of this problem, the present invention proposes a dual-path fluorescence light intensity method to detect the content of free hemoglobin in blood bags. Contents of the invention [0003] The invention provides a method for measuring the content of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 李刚张梦秋万兴华倪静林凌
Owner TIANJIN UNIV
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