Dual-light-path modulated transmitting and fluorescence excitation light source measurement method for ingredients of complicated solution

Abstract

The invention discloses a dual-light-path modulated transmitting and fluorescence excitation light source measurement method for ingredients of a complicated solution. The method comprises the steps: a modulating device is used for modulating a transmitting light source and a fluorescence excitation light source, and the transmitting light source and the fluorescence excitation light source are separately used for carrying out transmitting and exciting on the complicated solution in a packaging bag; a displacement platform controls movement of the light sources, and a spectrum receiving device acquires a transmitted spectrum and a fluorescence spectrum; a time sequence of each wavelength of each of the transmitted spectrum and the fluorescence spectrum is converted into a frequency domain, two transmitted spectra in the frequency domain and two fluorescence spectra in the frequency domain are constructed by using a fundamental component of each wavelength, a light intensity ratio of the two constructed transmitted spectra in the frequency domain at each wavelength is subjected to logarithm solving to obtain absorption spectra, and the absorption spectra and the two fluorescence spectra in the frequency domain are subjected to normalizing processing together and are compared with existing chemoanalytic results to establish a mathematical model; absorption spectra and fluorescence spectra in the frequency domain of an unknown bagged complicated solution are acquired by the same method, are normalized and then are interpolated into the mathematical model, so as to obtain the content of measured target ingredients in the complicated solution.

Description

technical field [0001] The invention relates to the field of concentration analysis stoichiometry of spectrally complex solutions, in particular to a method for measuring components of complex solutions by modulating transmission and fluorescence excitation light sources with dual optical paths. Background technique [0002] In the prior art, the relatively mature method is to detect the content of complex solution components in the packaging bag through chemical inspection, which has the outstanding advantage of high accuracy, but the method of chemical inspection cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and non-polluting characteristics, spectroscopic measurement may also realize the content detection of the target components measured in the complex solution in the packaging bag. [0003] In transmission spectrum detection, according to the Lambert-Beer law: the incident light intensity I of each wavelength is measured separate...

AI Technical Summary

Problems solved by technology

[0013] However, it is difficult to find exactly the same neutral attenuator in different occasions, and it is difficult to ensure that the position of the sample and the neutral attenuator are consistent, which increases the difficulty of measurement and makes it difficult to ensure measurement accuracy

[0014] In view of the complexity of the complex solution, the pure transmission spectrum is not pertinent. In order to further improve the measurement accuracy of the components of the complex solution, combined with the characteristics of strong pertinence of fluorescence, it is limited by the intensity of fluorescence excitation light, the length of the optical path and the concentration of the measured components. Affected, resulting in a serious self-absorption problem in fluorescence, resulting in a strong nonlinearity in the obtained fluorescence spectrum, which cannot well reflect the characteristics of the measured substance. At the same time, the traditional fluorescence spectrum cannot completely eliminate the influence of spectral background noise.

Images