Method for measuring free hemoglobin by double-optical-path multiposition intra-frequency-domain fluorescence light intensity

Abstract

The invention discloses a method for measuring free hemoglobin by double-optical-path multiposition intra-frequency-domain fluorescence light intensity. The method includes driving a fluorescence excitation light source by square wave signals, clinging a light outlet of a fluorescence excitation light source and an entrance slit of a light intensity receiving device to a blood bag, exciting a blood sample by the fluorescence excitation light source, and receiving fluorescence light intensity by the light intensity receiving device; controlling the fluorescence excitation light source to move to different positions and changing optical path measurement by a displacement platform, transforming the fluorescence light intensities collected at multiple positions under the two optical paths into a frequency domain, creating intra-frequency-domain fluorescence light intensities, combining with chemical testing data after normalization, and creating a mathematical model; collecting fluorescence light intensities of an unknown blood sample at multiple positions under the two optical paths, transforming the fluorescence light intensities into a frequency domain, creating intra-frequency-domain fluorescence light intensities, and inputting the fluorescence light intensities into the mathematical model after normalization to acquire the content of the free hemoglobin. The method has the advantages that influences of fluorescence background noise are eliminated, and light intensity nonlinearity caused by fluorescence self-absorption, blood scattering and the like is inhibited.

Description

technical field [0001] The invention relates to the field of stoichiometry for concentration analysis of light-intensity complex solutions, in particular to a method for measuring free hemoglobin with fluorescent light intensity in a dual optical path and multi-position frequency domain. Background technique [0002] In the existing technology, the more mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the chemical testing method needs to open the blood bag to take out the sample for testing, which cannot meet the needs of fast, fast and efficient testing. Non-contact and pollution-free requirements. [0003] The study found that fluorescence light intensity measurement is also possible to detect the content of free hemoglobin in blood bags due to its non-contact, non-polluting, and highly targeted characteristics. [0004] However, due to the influence of incide...

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Problems solved by technology

[0004] However, due to the influence of incident light intensity, optical path length and measured target concentration, the fluorescence has a serious self-absorption problem, as well as the scattering property of blood, which will lead to nonlinearity of light intensity, and cannot completely eliminate the influence of fluorescence background noise , Aiming at the above problems, this method proposes a method for measuring free hemoglobin by fluorescence light intensity in the frequency domain with dual optical paths and multiple positions

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