Preparation method of occult blood critical value quality control material

A preparation method and technology for quality control substances, which are applied in the preparation of test samples, biological testing, material inspection products, etc., can solve problems such as many raw materials, no commercial quality control substances, and unfavorable monitoring of reagent sensitivity.

Inactive Publication Date: 2013-04-17
许瑞娜
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 3. Diseases that can cause more red blood cells in the stool, such as dysentery, rectal polyps, hemorrhoids, etc., can also lead to positive reactions in occult blood tests
[0008] At present, most quantitative tests (such as the determination of alanine aminotransferase activity, etc.) have commercialized quality control substances, but there is no commercial quality control substance for fecal occult blood detection (gold calibration method), and it is difficult to carry out indoor quality control without self-preparation
In order to monitor the sensitivity of test reagents and prevent false negatives, weak positive samples are generally used as positive quality control substances in qualitative tests. A slight decrease in the sensitivity of test reagents can be reflected by using this quality control substance, and high-concentration quality control substances are not conducive to monitoring. Reagent sensitivity, and many raw materials used

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method for occult blood critical value quality control substance, comprising the steps of:

[0019] (1) Sealing treatment of the quality control container: fill up a 0.5ml centrifuge tube with 0.5% bovine serum albumin, place it in a refrigerator at 4°C, seal it for 24 hours, then discard the liquid in the centrifuge tube, Then place the centrifuge tube in a biological safety cabinet to dry naturally for later use;

[0020] (2) Preparation of critical quality control substances: Take a blood routine test specimen from a normal healthy person, according to the hemoglobin test result, hemolyze with distilled water and dilute to 1mg / L, add protein stabilizer and sodium azide to the final concentration of 2.0 g / L and 0.2g / L, then dispense into sealed 0.5ml centrifuge tubes, 50μl per tube;

[0021] (3) Storage of quality control substances: Store the aliquoted critical value quality control substances at 4°C.

[0022] Preferably, the centrifuge tube is a plas...

Embodiment 2

[0025] A preparation method for occult blood critical value quality control substance, comprising the steps of:

[0026] (1) Sealing treatment of the quality control container: fill up a 0.5ml centrifuge tube with 2.0% bovine serum albumin, place it in a refrigerator at 4°C, seal it for 24 hours, then discard the liquid in the centrifuge tube, Then place the centrifuge tube in a biological safety cabinet to dry naturally for later use;

[0027] (2) Preparation of critical quality control substances: Take a blood routine test specimen from a normal healthy person, according to the hemoglobin test result, hemolyze with distilled water and dilute to 2mg / L, add protein stabilizer and sodium azide to the final concentration of 7.0 g / L and 2.0g / L, then dispense into sealed 0.5ml centrifuge tubes, 500μl per tube;

[0028] (3) Storage of quality control substances: Store the aliquoted critical value quality control substances at -20°C.

[0029] Preferably, the centrifuge tube is a p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of an occult blood critical value quality control material, which comprises the following steps: (1) quality control material container sealing: filling a centrifugal tube with a specification of 0.5 ml by using 0.5-2.0 percent bovine serum albumin, putting into a refrigerator with a temperature of 4 DEG C, sealing for 24 hours, then abandoning liquid in the centrifugal tube and putting the centrifugal tube into a biological safety cabinet to naturally air for standby; (2) critical quality control material preparation: taking a blood routine test specimen of a normal healthy physical examinee, dissolving blood with distilled water according to hemoglobin test results, diluting into 1-2 mg/L, adding a protein stabilizer as well as sodium azide till the final concentration is 2.0-7.0 g/L and 0.2-2.0 g/L respectively and sub-packaging into 0.5 ml sealed centrifugal tube, wherein a solution in each tube is 50-500 ul; and (3) quality control material preservation: preserving the sub-packaged critical value quality control material at a temperature of 4-20 DEG C. The preparation method has the beneficial effects that the preparation is simple, whether a corresponding reagent deteriorates during the processes of circulation, preservation and use or not can be monitored, and the period of validity is long.

Description

【Technical field】 [0001] The invention relates to a preparation method of an indoor quality control substance, in particular to a preparation method of an occult blood critical value quality control substance. 【Background technique】 [0002] Occult blood in feces (also known as occult blood in feces) refers to a small amount of bleeding in the digestive tract, red blood cells are digested and destroyed, no abnormal changes in the appearance of feces, and bleeding that cannot be confirmed by naked eyes and microscopes. [0003] Clinical significance of detecting fecal occult blood: [0004] 1. In the early stage of gastrointestinal cancer, 20% of patients may have positive occult blood test, and the positive rate of occult blood test in advanced patients can reach more than 90%, and it can be persistently positive. Therefore, fecal occult blood test can be used as the first choice for screening gastrointestinal tumors . [0005] 2. Fecal occult blood test in patients with g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/96G01N1/38
Inventor 许瑞娜窦宇红杜纪坤华建江吴雄君徐志康丁华
Owner 许瑞娜
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap