Preparation method of occult blood critical value quality control material

Abstract

The invention provides a preparation method of an occult blood critical value quality control material, which comprises the following steps: (1) quality control material container sealing: filling a centrifugal tube with a specification of 0.5 ml by using 0.5-2.0 percent bovine serum albumin, putting into a refrigerator with a temperature of 4 DEG C, sealing for 24 hours, then abandoning liquid in the centrifugal tube and putting the centrifugal tube into a biological safety cabinet to naturally air for standby; (2) critical quality control material preparation: taking a blood routine test specimen of a normal healthy physical examinee, dissolving blood with distilled water according to hemoglobin test results, diluting into 1-2 mg / L, adding a protein stabilizer as well as sodium azide till the final concentration is 2.0-7.0 g / L and 0.2-2.0 g / L respectively and sub-packaging into 0.5 ml sealed centrifugal tube, wherein a solution in each tube is 50-500 ul; and (3) quality control material preservation: preserving the sub-packaged critical value quality control material at a temperature of 4-20 DEG C. The preparation method has the beneficial effects that the preparation is simple, whether a corresponding reagent deteriorates during the processes of circulation, preservation and use or not can be monitored, and the period of validity is long.

Description

【Technical field】 [0001] The invention relates to a preparation method of an indoor quality control substance, in particular to a preparation method of an occult blood critical value quality control substance. 【Background technique】 [0002] Occult blood in feces (also known as occult blood in feces) refers to a small amount of bleeding in the digestive tract, red blood cells are digested and destroyed, no abnormal changes in the appearance of feces, and bleeding that cannot be confirmed by naked eyes and microscopes. [0003] Clinical significance of detecting fecal occult blood: [0004] 1. In the early stage of gastrointestinal cancer, 20% of patients may have positive occult blood test, and the positive rate of occult blood test in advanced patients can reach more than 90%, and it can be persistently positive. Therefore, fecal occult blood test can be used as the first choice for screening gastrointestinal tumors . [0005] 2. Fecal occult blood test in patients with g...

AI Technical Summary

Problems solved by technology

[0006] 3. Diseases that can cause more red blood cells in the stool, such as dysentery, rectal polyps, hemorrhoids, etc., can also lead to positive reactions in occult blood tests

[0008] At present, most quantitative tests (such as the determination of alanine aminotransferase activity, etc.) have commercialized quality control substances, but there is no commercial quality control substance for fecal occult blood detection (gold calibration method), and it is difficult to carry out indoor quality control without self-preparation

In order to monitor the sensitivity of test reagents and prevent false negatives, weak positive samples are generally used as positive quality control substances in qualitative tests. A slight decrease in the sensitivity of test reagents can be reflected by using this quality control substance, and high-concentration quality control substances are not conducive to monitoring. Reagent sensitivity, and many raw materials used