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Collection liquid for protecting and stabilizing hemoglobin in manure and application of collection liquid

A technology of hemoglobin and collection fluid, applied in the field of biological applications, can solve the problems of reduced antigen titer and short storage time of feces collection fluid, and achieve the effect of long storage time

Active Publication Date: 2016-06-29
HANGZHOU ALLTEST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the biggest bottleneck in the detection of fecal occult blood in the market is that the stool collection liquid is not stored for a long time, especially for specimens with relatively low hemoglobin, the antigen titer decreases rapidly

Method used

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  • Collection liquid for protecting and stabilizing hemoglobin in manure and application of collection liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First add 70ml of purified water to the container, and then add 0.605g Tris Tris, 1g bovine serum albumin, 3.5g tetrasodium 3-hydroxy-2,2'iminodisuccinate (HIDS) in sequence , 1g ethylenediaminetetraacetic acid (EDTA), 2.5g polyethylene glycol, 0.02mlProclin150, 0.02mlProclin300, mixed evenly, and the volume was adjusted to 100mL, and the pH was adjusted to 9.4 to obtain the fecal occult blood collection solution.

Embodiment 2

[0026] First add 70ml of purified water to the container, then add 0.6g Tris, 0.5g bovine serum albumin, 1g tetrasodium 3-hydroxy-2,2'iminodisuccinate (HIDS) in sequence , 1.5g ethylenediaminetetraacetic acid (EDTA), 2.5g polyethylene glycol, 0.01gProclin150, 0.01gProclin300, mixed evenly, and then the volume was adjusted to 100mL, and the pH was adjusted to 9.4 to obtain the fecal occult blood collection solution.

Embodiment 3

[0028] First add 70ml of purified water to the container, then add 1.2g of disodium hydrogen phosphate, 0.5g of casein, 2g of 3-hydroxy-2,2' tetrasodium iminodisuccinate (HIDS), 2g of sodium tripolyphosphate , 3g polyethylene glycol, 0.5gProclin150, 0.5gProclin300, mixed evenly, and the volume was adjusted to 100mL, and the pH was adjusted to 9.5 to obtain the fecal occult blood collection solution.

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Abstract

The invention discloses collection liquid for protecting and stabilizing hemoglobin in manure and application of the collection liquid. The collection liquid comprises 25-45 g / L of chelating agent, 0.2-1 g / L of preservative, 1-10 g / L of sealing agent, 6-12 g / L of buffering agent and 25-30 g / L of polyethylene glycol, wherein the chelating agent is a mixture of two of HIDS, EDTA and sodium tripolyphosphate. After fresh manure and the collection liquid are mixed well according to a proportion of 50 mg:2 ml, the collection liquid can be stored for 7 days at normal temperature, one month at 2-8 DEG C and six months at minus 20 DEG C. The collection liquid can further stabilize hemoglobin and prolong storage time of hemoglobin in manure in protection liquid.

Description

technical field [0001] The invention belongs to the technical field of biological applications, in particular to a collection solution for protecting and stabilizing hemoglobin in feces and its application. Background technique [0002] "Fecal occult blood" refers to the daily gastrointestinal bleeding less than 5ml. There are many diseases such as: colorectal cancer, colitis, peptic ulcer, intestinal polyps, gastrointestinal diverticulum and other diseases have no symptoms in the early stage, only fecal occult blood. Early colorectal cancer refers to those whose lesions are limited to the mucosa or submucosa without invading the muscle layer. They are often asymptomatic and do not actively seek medical advice. Screening of high-risk groups and regular inspections are important ways to detect early cancer patients. Clinical studies have confirmed that 87% of patients with gastrointestinal tumors have a positive fecal occult blood test; trace bleeding and trace hemoglobin in ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor V·马迪高飞陆维克陈金树
Owner HANGZHOU ALLTEST BIOTECH
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