Serum creatinine detecting reagent

A technology for detecting reagents and serum creatinine, which is applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of unsatisfactory detection effect, weak anti-interference ability, low precision and accuracy, etc. Strong anti-interference ability and sensitivity, the effect of high analytical sensitivity

Active Publication Date: 2015-03-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing enzymatic creatinine determination reagents on the market have weak anti-

Method used

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  • Serum creatinine detecting reagent
  • Serum creatinine detecting reagent
  • Serum creatinine detecting reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] A serum creatinine detection reagent comprises reagent R1 and reagent R2, and adopts liquid double reagent colorimetric method to test the content of creatinine in serum, and the main components of the reagent are as follows:

[0073] Reagent R1 consists of:

[0074] Tris buffer (pH7.8) 10mmol / L

[0075] Creatinase 2KU / L

[0076] Sarcosine oxidase 6KU / L

[0077] Ascorbate oxidase 1KU / L

[0078] Lipase 1KU / L

[0079] Lipoprotein lipase 1KU / L

[0080] Bilirubin oxidase 1KU / L

[0081] γ-Fe 2 o 3 Nanoparticles 10mmol / L

[0082] TOOS0.1mmol / L

[0083] 4-Aminoantipyrine 0.1mmol / L

[0084] Potassium ferrocyanide 10μmol / L

[0085] BSA 0.1g / L

[0086] Sucrose 5g / L

[0087] Peroxidase 1.5KU / L

[0088] Tween-20 0.8g / L

[0089] Sodium azide 1g / L;

[0090] Reagent R2 consists of:

[0091] Tris buffer (pH7.8) 10mmol / L

[0092] Creatinase 55KU / L

[0093] BSA 0.5g / L

[0094] Sucrose 5g / L

[0095] Tween-20 0.8g / L

[0096] Sodium azide 1g / L.

[0097] γ-Fe in th...

Embodiment 2

[0099] A serum creatinine detection reagent comprises reagent R1 and reagent R2, the main components of which are as follows:

[0100] Reagent R1 consists of:

[0101] Tris buffer (pH7.8) 45mmol / L

[0102] Creatinase 18KU / L

[0103] Sarcosine oxidase 15KU / L

[0104] Ascorbate oxidase 15KU / L

[0105] Lipase 10KU / L

[0106] Lipoprotein lipase 10KU / L

[0107] Bilirubin oxidase 10KU / L

[0108] Nanoparticles 50mmol / L

[0109] TOOS0.6mmol / L

[0110] 4-Aminoantipyrine 0.4mmol / L

[0111] Potassium ferrocyanide 20μmol / L

[0112] BSA 3g / L

[0113] Sucrose 20g / L

[0114] Peroxidase 3KU / L

[0115] Tween-20 1.5g / L

[0116] Sodium azide 3g / L;

[0117] Reagent R2 consists of:

[0118] Tris buffer (pH7.8) 45mmol / L

[0119] Creatinase 80KU / L

[0120] BSA 3g / L

[0121] Sucrose 20g / L

[0122] Tween-20 1.5g / L

[0123] Sodium azide 3g / L.

[0124] γ-Fe in the above reagent R1 2 o 3 The particle size of nanoparticles is 20-50nm.

Embodiment 3

[0126] A serum creatinine detection reagent comprises reagent R1 and reagent R2, the main components of which are as follows:

[0127] Reagent R1 consists of:

[0128] Tris buffer (pH7.8) 40mmol / L

[0129] Creatinase 16KU / L

[0130] Sarcosine oxidase 8KU / L

[0131] Ascorbate oxidase 5KU / L

[0132] Lipase 4KU / L

[0133] Lipoprotein lipase 5KU / L

[0134] Bilirubin oxidase 3KU / L

[0135] Nanoparticles 40mmol / L

[0136] TOOS0.4mmol / L

[0137] 4-Aminoantipyrine 0.13mmol / L

[0138] Potassium ferrocyanide 20μmol / L

[0139] BSA 1g / L

[0140] Sucrose 10g / L

[0141] Peroxidase 2KU / L

[0142] Tween-20 1g / L

[0143] Sodium azide 1.5g / L;

[0144] Reagent R2 consists of:

[0145] Tris buffer (pH7.8) 40mmol / L

[0146] Creatinase 60KU / L

[0147] BSA 1g / L

[0148] Sucrose 10g / L

[0149] Tween-20 1g / L

[0150] Sodium azide 1.5g / L.

[0151] γ-Fe in the above reagent R1 2 o 3 The particle size of nanoparticles is 20-30nm.

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Abstract

The invention discloses a serum creatinine detecting reagent, and belongs to the technical field of clinical in-vitro detection. According to the serum creatinine detecting reagent, gamma-Fe2O3 nano-particles are added into a reagent R1, so that the activity of peroxidase and the specificity to a substrate are enhanced, and the interferences of reducing substances, such as ascorbic acid, uric acid, glutathione and bilirubin, are reduced; meanwhile, the gamma-Fe2O3 nano-particles also serve as a catalyst of a reaction; the interference of endogenous creatine is effectively eliminated by the combination action of the gamma-Fe2O3 nano-particles and the peroxidase. In addition, the serum creatinine detecting reagent adopts N-ethyl-N-(2-hydroxy-3-sulfonated propyl)-3-methylaniline sodium salt (TOOS) as a chromogen, so that the reagent is more stable; the analysis sensitivity is high. The substances, such as lipase and lipoprotein lipase, are also added into the reagent R1, so that the influence of lipid turbidity can be removed effectively; therefore, the reagent of the invention has higher anti-jamming capability and stability.

Description

technical field [0001] The invention relates to a detection reagent for clinical determination of creatinine content in serum, belonging to the technical field of clinical in vitro detection. Background technique [0002] Serum creatinine (Cr), also known as blood creatinine, is generally considered to be endogenous blood creatinine, which is a product of human muscle metabolism. In muscle, creatine is mainly slowly formed into creatinine through irreversible non-enzymatic dehydration reaction, and then released into the blood and excreted with urine. Therefore, serum creatinine is closely related to the total amount of muscle in the body and is not easily affected by diet. Creatinine is a small molecular substance that can be filtered by the kidneys and is rarely absorbed in the renal tubules. Almost all creatinine produced in the body is excreted with urine and is generally not affected by urine output. Serum creatinine detection is one of the main methods commonly used ...

Claims

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Application Information

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IPC IPC(8): G01N33/70
CPCG01N33/54393G01N33/70
Inventor 谭柏清李志明甘宜梧王绮李静谢清华赵新
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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