Construction of a comparative database and identification of virulence factors through comparison of polymorphic regions in clinical isolates of infectious organisms

Abstract

The present invention is directed to novel nucleotide sequences to be used for diagnosis, identification of the strain, typing of the strain and giving orientation to its potential degree of virulence, infectivity and / or latency for all infectious diseases more particularly tuberculosis. The present invention also includes method for the identification and selection of polymorphisms associated with the virulence' and / or infectivity in infectious diseases more particularly in tuberculosis by a comparative genomic analysis of the sequences of different clinical isolates / strains of infectious organisms. The regions of polymorphisms, can also act as potential drug targets and vaccine targets.; More particularly, the invention also relates to identifying virulence factors of M. tuberculosis strains and other infectious organisms to be included in a diagnostic DNA chip allowing identification of the strain, typing of the strain and finally giving orientation to its potential degree of virulence. Although the present invention has been illustrated with specific reference to the polymorphic region in the Mycobacterium tuberculosis, the said invention is not to be understood and construed as being limited to Tuberculosis but is applicable to all infectious diseases.

Description

field of invention The present invention relates to a new nucleotide sequence, which will be used for diagnosis, strain identification, strain typing and guidance for potential toxicity degree, infectivity and / or incubation period of all infectious diseases including tuberculosis. The present invention also includes methods of identifying and selecting polymorphisms associated with virulence and / or infectivity in infectious diseases by comparative genomic analysis of the sequences of different clinical isolates / strains of infectious organisms. Polymorphic regions can also serve as potential drug targets and vaccine targets. More specifically, the present invention also relates to the identification of virulence agents for M. tuberculosis strains and other infectious organisms to be included in diagnostic DNA chips, allowing identification of strains, typing of strains and ultimately their potential virulence degree guidance. Although the invention has been elucidated with pa...

AI Technical Summary

Problems solved by technology

There are no data or technical information in the prior art to allow the specific selection of potential new targets and protective antigens for new drug and vaccine compositions for the treatment and prevention of infectious diseases, especially tuberculosis

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