Attenuated, brightened and replication-controllable HSV recombinant virus, preparation method and applications thereof

A technology for recombining viruses and viral genomes, applied in the directions of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of high toxicity and weak sensitivity, and achieve the effect of reducing toxicity and widening the host range.
CN107630009AInactive Publication Date: 2018-01-26WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
Publication Date
2018-01-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses attenuated, brightened and replication-controllable HSV recombinant virus, a preparation method and applications thereof. According to the present invention, thymidine kinase (TK) gene essential for replicating viruses in neurons and being a main virulence factor is knocked out by using a homologous recombination method, and subsequently a red or green fluorescent gene enhancement expression cassette is recombined into the genome of the virus to construct a series of novel recombinants viruses, wherein the toxicity is markedly low, the states of infected mice are good,the fluorescence signal is strong, and the expression of the recombinant viruses is limited at the injection site after the recombinant viruses are used in in-vivo animal center labeling; by combiningwith Cre-dependent AAV helper viruses capable of expressing TK in a compensated manner, the cell-specific transmonosynaptic loop tracing is achieved; and the recombinant HSV has wide application value in nervous system targeted gene transduction, neural network transsynaptic tracing, tumor disintegration, viral replication and pathogenesis mechanism, antiviral drug screening and other fields.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to an attenuated, brightened, and controllable replication HSV recombinant virus and its preparation method and application, and relates to the construction of an AAV helper virus system that assists HSV recombinant virus to trace across a single synapse, and the Application of tool virus system in anterograde transmonosynaptic tracing of brain neural circuits, nervous system targeted gene transduction and oncolytic therapy. Background technique

[0002] The brain is the most complex organ in the human body, yet we still know very little about it. It is the dream of scientists to reveal the mechanism of the brain. In 2013, the "Brain Project" of the United States and the European Union was launched successively, setting off an upsurge in brain science research. Studying the structure of neural networks is an important basis for understanding the working mechanism of the bra...

Claims

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