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Muricauda olearia and application thereof

An oil-resistant and stalked technology, applied in applications, bacteria, chemicals for biological control, etc., can solve problems such as antibiotics accelerating bacterial resistance, improve resistance, reduce the secretion of virulence factors, and reduce synthetic drugs. Effect

Active Publication Date: 2014-08-13
OCEAN UNIV OF CHINA
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Problems solved by technology

The overuse of antibiotics has further accelerated the emergence of bacterial resistance

Method used

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  • Muricauda olearia and application thereof
  • Muricauda olearia and application thereof
  • Muricauda olearia and application thereof

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Embodiment Construction

[0019] The strains of the present invention will be described in detail below with reference to the examples.

[0020] 1. Isolation of P. oleifera LMM001 strain

[0021] In March 2011, the body surface mucus of healthy flounder from the farm was coated on the marine bacterial culture medium 2216E plate, and yellow colony bacteria were found after 2 days of cultivation at 28°C, and a single colony was picked for subculture until the purified strain, and the target strain was obtained by screening the efficiency of AHL degradation activity, named LMM001.

[0022] The strain LMM001 of the present invention was determined to be Muricauda olearia through 16s rRNA gene comparison analysis.

[0023] 2. Detection of AHL degradation activity of P. oleifera LMM001 strain

[0024] First inoculate LMM001 in seawater 2216E medium, and culture on a shaker at 170 rpm at 28°C for 24 hours. AHL molecules (C6-C14-HSL) with different carbon chain lengths were added to the cultured bacterial l...

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Abstract

The invention provides Muricauda olearia. Muricauda oleariah is a quorum sensing quenching strain capable of inhibiting the secretion of pathogenic bacteria virulence factors, and the preservation number of Muricauda olearia LMM001 strain is CGMCC No. 8972. The invention also provides an AHL degrading enzyme MomL protein separated from the LMM001 strain, wherein the amino acid sequence of the MomL protein is SEQ ID NO: 1. The Muricauda olearia LMM001 strain disclosed by the invention can block the QS pathway of pathogenic bacteria by small molecule QS repressor, AHL lactonase MomL, AHL acyltransferase and other ways, and reduce the secretion of pathogenic bacteria virulence factors; the LMM001 strain has no toxic and side effects on zebrafish and can improve the resistance of zebrafish to the infection of Aeromonas hydrophila; AHL lactonase can greatly reduce the secretion of virulence factors such as Pseudomonas aeruginosa extracellular protease and pyocyanine and can reduce the synthesis of Chromobacterium violaceum and violacein.

Description

technical field [0001] The invention belongs to the technical field of beneficial bacteria screening, and in particular relates to a oleifera oleifera and application thereof. Background technique [0002] At present, antibiotics are recognized as effective methods for the treatment of bacterial diseases. Traditional antibiotics mainly kill bacteria by inhibiting the synthesis or metabolism of some microorganisms, such as cell wall synthesis, DNA replication, RNA transcription, and protein synthesis. Or inhibit the reproduction of bacteria, so as to achieve the purpose of treating bacterial diseases. This "live" or "dead" selective pressure keeps the antibiotic resistance genes produced by random mutations, which leads to the rapid emergence of antibiotic-resistant mutant bacteria as the dominant population in the microbial community, which in turn leads to the emergence of bacterial resistance . The overuse of antibiotics has further accelerated the emergence of bacterial...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A23K1/16A23K1/165C02F3/34C12N9/18C12N15/55C12R1/01A23K10/18
Inventor 张晓华汤开浩史晓翀苏颖张蕴慧
Owner OCEAN UNIV OF CHINA
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