Aeromonas hydrophila inactivated vaccine and preparation thereof

A technology of Aeromonas hydrophila and inactivated vaccine, which is applied in the field of Aeromonas hydrophila inactivated vaccine and its preparation, can solve the problems of poor safety, high cost, unstable immune effect and the like, and reduce mortality rate , the effect of reducing economic losses

Inactive Publication Date: 2010-02-10
张秀军 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the disease prevention effect of these vaccines has greatly surpassed other control measures such as antibiotics and chemicals, but these vaccines also have some problems

Method used

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  • Aeromonas hydrophila inactivated vaccine and preparation thereof
  • Aeromonas hydrophila inactivated vaccine and preparation thereof
  • Aeromonas hydrophila inactivated vaccine and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Isolate the pathogenic bacteria Aeromonas hydrophila from sick fish suffering from bacterial septicemia:

[0034] a. Aseptically take the liver, spleen and kidney of diseased or dying carp, inoculate them on normal nutrient agar medium by streaking, culture at 28°C for 24 hours, pick a single colony to obtain a pure culture, and transfer it to the normal nutrient agar slant Stored on culture medium for identification. Typical colonies on ordinary nutrient agar plates are round, colorless or light yellow, translucent, smooth, moist, with neat edges and no water-soluble pigments.

[0035] b. Take the pure cultured bacteria on the slant above, make a smear specimen, and check the bacterial morphology by Gram staining microscope. The results of ordinary light microscope examination are Gram-negative short bacilli, with blunt round or straight ends, most of which are arranged in single, and a few are arranged in two, without capsule and without spores. Then inoculated ...

Embodiment 2

[0072] This embodiment is basically the same as Embodiment 1, except that the liquid vaccine is made into a solid powder vaccine by a freeze dryer.

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Abstract

The invention relates to a fish vaccine and a preparation method thereof, in particular to an aeromonas hydrophila inactivated vaccine and the preparation thereof. The aeromonas hydrophila inactivatedvaccine is prepared by the following concrete steps: separating out a pathogen from a sick fish, determining the pathogen as an aeromonas hydrophila through morphology, virulence factors, biochemicalreaction and gene sequencing; rejuvenating the strain, selecting an optimum condition for cultivation; then selecting the optimum condition for inactivation to prepare the aeromonas hydrophila inactivated vaccine, and conforming the aeromonas hydrophila inactivated vaccine to be certificated through sterility test and security test. The inactivated vaccine is prepared from a pathogenic bacterialstrain separated from a sick fish body, has a simple operating method, strong pertinency, strong protective function, good immunological effect and low cost of the used material, and is suitable for mass production. The invention provides a practical method for preventing and curing the bacteremic septicemia of carps.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to an inactivated Aeromonas hydrophila vaccine and its preparation. Background technique [0002] Aeromonas hydrophila widely exists in fresh water, soil, sewage, sludge and feces, and can be divided into pathogenic strains and non-pathogenic strains. The infection spectrum of pathogenic strains is very wide, and it is the main pathogenic bacteria of freshwater fish. Bacterial septicemia caused by this bacterium is one of the most harmful fish types in the history of fish farming in my country, the largest age range of harmful fish, the widest endemic area, the longest endemic season, the most types of fish farming waters, and the most serious losses. an acute infectious disease. The disease is currently mainly controlled by antibiotics and chemical drugs, but long-term use of antibiotics is easy to produce drug resistance, chemical drugs will cause residues, pollute water quality, and destroy eco...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04
Inventor 张秀军张玉芬史相国
Owner 张秀军
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